U6 is not a suitable endogenous control for the quantification of circulating microRNAs

Xiang, Mengqin; Zeng, Ying; Yang, Ruirui; Xu, Hao; Chen, Zhuo; Zhong, Jing; Xie, Huixu; Xu, You‐Ping; Zeng, Xi

doi:10.1016/j.bbrc.2014.10.064

Cited by 201 publications

(162 citation statements)

References 43 publications

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“…Second, the internal reference for circulating microRNAs tests is still an issue. For instance, U6, which is usually used as an internal reference for circulating microRNA test, is not a suitable one as suggested by some study (32).…”

Section: Possibility Of Mir-499 As a Biomarker For Diagnosis Of Amimentioning

confidence: 99%

Circulating miR-499 as a potential biomarker for acute myocardial infarction

Xin¹,

Yang²,

Han³

2016

Ann. Transl. Med.

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Acute myocardial infarction (AMI), a common heart disease that may lead to chronic heart failure, is the leading cause of morbidity and mortality worldwide. MicroRNAs (miRNAs) are small non-coding RNAs that mediate the expression of target genes. Recently, a number of miRNAs are emerging as potential biomarkers of AMI. MiRNA-499 is a newly discovered member of miRNAs, and is mainly expressed in myocardium, the circulating levels of miRNA-499 was increased in AMI patients. This review summarizes the latest advances in the miRNA-499 study and discusses the potential of miRNA-499 to be a biomarker of AMI. Acute myocardial infarction (AMI) is one of the leading causes of morbidity and mortality worldwide, thus an effective and accurate diagnostic biomarker would be needed to help decrease the mortality of AMI patients. One of the traditional biomarkers for AMI is troponin. Circulating troponin levels rise around 3 hours after the onset of chest pain, due to the relative delayed release time of troponin. It is widely believed that a rapid and correct diagnosis of AMI has an important impact on patients' treatment and prognosis. Therefore, earlier biomarkers than troponin with both high sensitivity and specificity remain to be needed. MicroRNAs (miRNAs) are endogenous small non-coding RNAs of less than 22 nucleotides that are implicated in nearly all cellular events including cell differentiation and proliferation as well as the pathogenesis of certain human diseases (1,2). Recently, a number of findings support the notion of the implication of miRNAs in the development and progression of cardiovascular diseases (3). The plasma levels of miRNA-1, -133a, -133b, and -499-5p are upregulated in AMI patient, and represent novel biomarkers of cardiac damage. Several reviews have provided an over-view of biology of miRNA-1, miRNA-133b, -499-5p and their involvement in cardiovascular physiology and pathology, and discussed the potential of these miRNAs to become a novel biomarker of AMI and even an effective therapeutics agent (4-8). miRNA-499 is a newly discovered member of miRNAs encoded by myosin gene family, and its expression level in plasma was elevated in the patients with AMI, a common heart disease that is the leading cause of morbidity and mortality worldwide (9). A previous study compared the diagnostic performance of miRNA-499 and some traditional biomarkers like SMB, cTnI, cTnT, CK-MB, CK, LDH, and concluded that miRNA-499 was present in plasma earlier than other conventional biomarkers of AMI, pointing to the likelihood that miR-499 can be a vital molecule in the early diagnosis of AMI. This study also found that the circulating level of miR-499 correlated well with circulating troponin I

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Section: Possibility Of Mir-499 As a Biomarker For Diagnosis Of Amimentioning

confidence: 99%

Circulating miR-499 as a potential biomarker for acute myocardial infarction

Xin¹,

Yang²,

Han³

2016

Ann. Transl. Med.

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show abstract

“…Mean Ct values were calculated for each sample and normalized against miR-16 to obtain dCT values. MiR-16-5p was used as endogenous control to normalize miR expression levels based on prior publications (43,(51)(52)(53)(54)(55) as well as available TCGA thyroid data, where miR-16 appears as relatively invariant within thyroid tumor samples (12). Mean dCt values relative to expression of miR-16 were normally distributed in the data set.…”

Section: Mir Analysismentioning

confidence: 99%

Association of BRAF^V600E Mutation and MicroRNA Expression with Central Lymph Node Metastases in Papillary Thyroid Cancer: A Prospective Study from Four Endocrine Surgery Centers

Han

Kim

Cho

et al. 2016

Thyroid

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“…Of note, this study used another platform for miR analysis (Taqman qPCR assay) and normalized against U6 [34]. Several studies has in the last years concluded that U6 is not a suitable endogenous control for the quantification of circulating miRs [66,67].…”

mentioning

confidence: 99%

Circulating microRNAs predict future fatal myocardial infarction in healthy individuals – The HUNT study

Bye

Røsjø

Nauman

et al. 2016

Journal of Molecular and Cellular Cardiology

107

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U6 is not a suitable endogenous control for the quantification of circulating microRNAs

Cited by 201 publications

References 43 publications

Circulating miR-499 as a potential biomarker for acute myocardial infarction

Circulating miR-499 as a potential biomarker for acute myocardial infarction

Association of BRAF^V600E Mutation and MicroRNA Expression with Central Lymph Node Metastases in Papillary Thyroid Cancer: A Prospective Study from Four Endocrine Surgery Centers

Circulating microRNAs predict future fatal myocardial infarction in healthy individuals – The HUNT study

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U6 is not a suitable endogenous control for the quantification of circulating microRNAs

Cited by 201 publications

References 43 publications

Circulating miR-499 as a potential biomarker for acute myocardial infarction

Circulating miR-499 as a potential biomarker for acute myocardial infarction

Association of BRAFV600E Mutation and MicroRNA Expression with Central Lymph Node Metastases in Papillary Thyroid Cancer: A Prospective Study from Four Endocrine Surgery Centers

Circulating microRNAs predict future fatal myocardial infarction in healthy individuals – The HUNT study

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Association of BRAF^V600E Mutation and MicroRNA Expression with Central Lymph Node Metastases in Papillary Thyroid Cancer: A Prospective Study from Four Endocrine Surgery Centers