U.S. Standard Bioassay for the Potency Assessment of Bacillus thuringiensis Serotype H-14 Against Mosquito Larvae

McLaughlin, Ronald; Dulmage, Howard T.; Alls, R. T.; Couch, T. L.; Dame, David A.; Hall, I. M.; Rose, Robert I.; Versoi, P. L.

doi:10.1093/besa/30.1.26

Cited by 24 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples were coated with gold in a Fullam EMS-76 M evaporator for 9 min and examined and photographed with a Philips XL30-ESEM scanning electron microscope operating at a voltage of 10 to 15 kV at ϫ2,000 and ϫ18,000 magnifications. Insecticidal activity of B. thuringiensis strains was screened on larvae of the three mosquito species as described previously (10). Twenty early fourth-instar larvae were placed in 100 ml of dechlorinated water.…”

Section: Methodsmentioning

confidence: 99%

Diversity of Bacillus thuringiensis Strains from Latin America with Insecticidal Activity against Different Mosquito Species

Ibarra

Rincón

Ordúz

et al. 2003

Appl Environ Microbiol

130

View full text Add to dashboard Cite

The characterization of selected Bacillus thuringiensis strains isolated from different Latin America countries is presented. Characterization was based on their insecticidal activity against Aedes aegypti, Culex quinquefasciatus, and Anopheles albimanus larvae, scanning electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and plasmid profiles as well as PCR analysis using novel general and specific primers for cry and cyt genes encoding proteins active against mosquitoes (cyt1, cyt2, cry2, cry4A, cry4B, cry10, cry11, cry17, cry19, cry24, cry25, cry27, cry29, cry30, cry32, cry39, and cry40). Strains LBIT315, LBIT348, and IB604 showed threefold higher mosquitocidal activity against A. aegypti and C. quinquefasciatus larvae than B. thuringiensis subsp. israelensis and displayed high similarities with the B. thuringiensis subsp. israelensis used in this study with regard to protein and plasmid profiles and the presence of cry genes. Strain 147-8906 has activity against A. aegypti similar to that of B. thuringiensis subsp. israelensis but has different protein and plasmid profiles. This strain, harboring cry11, cry30, cyt1, and cyt2 genes, could be relevant for future resistance management interventions. Finally, the PCR screening strategy presented here led us to identify a putative novel cry11B gene.

show abstract

Section: Methodsmentioning

confidence: 99%

Diversity of Bacillus thuringiensis Strains from Latin America with Insecticidal Activity against Different Mosquito Species

Ibarra

Rincón

Ordúz

et al. 2003

Appl Environ Microbiol

130

View full text Add to dashboard Cite

show abstract

“…To assure that the MPCA preparations used in the clam larval toxicity and pathogenicity test possessed activity against a target organism, the following tests were performed. The potency of Bti was determined by a toxicity test against 3rd instar Aedes aegypti as described by McLaughlin et al (1984). The molluscicidal toxicity of Bacillus alvei III3DTiA was determined using the snail Biornphalaria glabrata (Singer et al 1994).…”

Section: Methodsmentioning

confidence: 99%

Nontarget testing of microbial pest control agents using larvae of the coot clam Mulinia lateralis

Nm¹,

S²,

Fj³

1996

Dis. Aquat. Org.

View full text Add to dashboard Cite

A short-term (48 h ) chemical toxlcity test using larvae of the coot clam A4ulinia lateralis was modified to evaluate potential toxlcity and pathogenicity of microblal pest control agents. M. lateralls larvae, at the straight-hinged stage of developn~ent, were exposed to varlous microbial pest control agents including a mosquito larvac~de. Bacillus thur~ngiensis var israelensis (Bti); a mol1usc1-cidal strain of Bacillus alvei; a viral pathogen of the gypsy moth Lymantria dispar nuclear polyhedrosis virus (LdNPV); and a broad host-range fungal insect pathogen, Metarhizium anisopliae. Mortalities significantly h~g h e r than heat-killed controls were obtained w~t h Bti at a 10-4 dilution of a colnlnei-cial preparatlon, and w~t h LdNPV at an occlus~on body d e n s~t y of 1 X 10%1-' Sodlum dodecyl sulfate (SDS) and the ivater-soluble fraction of No. 2 fuel 011 ( W S r ) were also tested to provide a measure of comparison, sensitlv~ty and precision. SDS, toxic at an LC,, of 6.3 m g 1-', had a mean coefficient of variation of 23'X). The clam larval tox~city test was very sensitive to WSF,,,,, exposures resulted In an LC.,,, of <10% V/\! Because of its preclslon, sensitivity and simplicity, the M. lateralis larval test has the potential to be useful for assessing adverse effects that microblal pest control agents may have on nontarget bivalves KEY WORDS: B~o l o g~c a l control Nontarget effects . Mullnja lateralis

show abstract

“…The determination method was based on the number of international units per milligram (IU/mg), using bioassay test and the calculation formula according to McLaughlin et al (1984) as follows:…”

Section: Determination the Active Ingredient Of Samplesmentioning

confidence: 99%

Influence of some environmental conditions on stability and activity of Bacillus thuringiensis formulations against the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

Moustafa

Saleh

Ateya

et al. 2018

Egypt J Biol Pest Control

View full text Add to dashboard Cite

The entomopathogenic bacterium Bacillus thuringiensis (Bt) has been used in crop protection for the last 70 years; however, many environmental conditions affect its activity. The present study was directed to evaluate the influence of certain environmental conditions on stability and activity of Bt samples of the two commercial formulations (Dipel 2 × 6. 4% WP and Protecto 9.4% WP), when stored under accelerated hot storage, shelf, and outdoor storage. Photo degradation of the two formulations was studied in aqueous solution. The results revealed that the loss percentage of Protecto formulation was above the permissible limits of WHO specifications after 2 years of storage at ambient conditions and the thermal stability of the Bt was affected negatively depending on the storage periods. In accordance with this trend, the bioassay tests versus neonate and second instar larvae of the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), showed a decrease in toxicity of the formulations to (60%) after storage at 35 ± 2°C for 12 weeks and about (70%) after storage under sunlight for 2 days. Photolysis of aqueous solutions reduced the half-life of formulations by about 1-2 days. The stability of Bt should be evaluated prior to submission for registration as these products have showed less stability under storage at ambient conditions. The stakeholders and decision-makers can utilize these results and examine such products case by case.

show abstract

U.S. Standard Bioassay for the Potency Assessment of Bacillus thuringiensis Serotype H-14 Against Mosquito Larvae

Cited by 24 publications

References 0 publications

Diversity of Bacillus thuringiensis Strains from Latin America with Insecticidal Activity against Different Mosquito Species

Diversity of Bacillus thuringiensis Strains from Latin America with Insecticidal Activity against Different Mosquito Species

Nontarget testing of microbial pest control agents using larvae of the coot clam Mulinia lateralis

Influence of some environmental conditions on stability and activity of Bacillus thuringiensis formulations against the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

Contact Info

Product

Resources

About