U-phosphate biomineralization induced by Bacillus sp. dw-2 in the presence of organic acids

Tu, Hong; Yuan, Guoyuan; Zhao, Changsong; Li, Jun; Li, Feize; Yang, Jijun; Liao, Jiali; Yang, Yuanyou; Liu, Ning

doi:10.1016/j.net.2019.03.002

Cited by 33 publications

(16 citation statements)

References 62 publications

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“…The concentration of dissolved U(VI) was analyzed by a colorimetric technique by Tu et al (2019) , using a UV-Vis spectrophotometer TU-1810 (Beijing Persee, China). In brief, 1 ml sample was mixed with 1 ml of 0.05% Arsenazo(III) and 8 ml chloroacetic acid–sodium acetate (CH 2 ClCOOH–CH 3 COONa) buffer.…”

Section: Methodsmentioning

confidence: 99%

“…Since then, various bacteria, including Bacillus sp. ( Tu et al, 2019 ), Rahnella sp. ( Beazley et al, 2009 ), Rhodanobacter A2-61 ( Sousa et al, 2013 ), Pseudomonas aeruginosa ( Choudhary and Sar, 2011 ), and Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

“…However, the mechanisms of U(VI) immobilization by these microorganisms are inconsistent in a number of existing studies. A previous study ( Tu et al, 2019 ) showed that U could be biomineralized on Bacillus sp. dw-2 as UO 2 HPO 4 ⋅4H 2 O or (UO 2 ) 3 (PO 4 ) 2 ⋅4H 2 O, while another research implied that U(VI) was initially adsorbed on the B. thuringiensis surface and then stabilized by the formation of needle-like amorphous U compounds ( Pan et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Identification of Uranium Tolerant Phosphate-Solubilizing Bacillus spp. and Their Synergistic Strategies to U(VI) Immobilization

Zhong

Liu

et al. 2021

Front. Microbiol.

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The remediation of uranium (U) through phosphate-solubilizing bacteria (PSB) is an emerging technique as well as an interesting phenomenon for transforming mobile U into stable minerals in the environment. While studies are well needed for in-depth understanding of the mechanism of U(VI) immobilization by PSB. In this study, two PSB were isolated from a U-tailing repository site. These bacterial strains (ZJ-1 and ZJ-3) were identified as Bacillus spp. by the sequence analysis of 16S ribosomal RNA (rRNA) genes. Incubation of PSB in liquid medium showed that the isolate ZJ-3 could solubilize more than 230 mg L–1 P from glycerol-3-phosphate and simultaneously removed over 70% of 50 mg L–1 U(VI) within 1 h. During this process, the rapid appearance of yellow precipitates was observed. The microscopic and spectroscopic analysis demonstrated that the precipitates were associated with U-phosphate compound in the form of saleeite-like substances. Besides, scanning electron microscopy coupled with energy-dispersive X-ray (SEM-EDS) and Fourier transform infrared spectroscopy (FTIR) analysis of the precipitates confirmed that the extracellular polymeric substances (EPS) might also play a key role in U sequestration. Furthermore, SEM and FTIR analysis revealed that part of U(VI) was adsorbed on the bacterial surface through cellular phosphate, hydroxy, carboxyl, and amide groups. This study provides new insights into the synergistic strategies enhancing U immobilization rates by Bacillus spp. that uses glycerol-3-phosphate as the phosphorus source, the process of which contributes to harmful pollutant biodegradation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and Identification of Uranium Tolerant Phosphate-Solubilizing Bacillus spp. and Their Synergistic Strategies to U(VI) Immobilization

Zhong

Liu

et al. 2021

Front. Microbiol.

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“…The presence of cations can entail variations in the chemical equilibrium during the enzymatic reductive precipitation due to the interaction or competitiveness with other cations and the metals to be recovered. For example, the addition of ammonia to wastewater with uranyl and phosphate may result in the formation of NH4UO2PO4, which has a lower solubility than HUO2PO4, and hence, enhancing the precipitation of uranyl (Tu et al, 2019).…”

Section: Enzymatic Reductive Precipitationmentioning

confidence: 99%

“…Strong ligands, such as oxalate or citrate, affects the enzymatic reductive precipitation due to the complexation of the metals. The effect of the organic ligands over the enzymatic reductive precipitations depends on the organic compound concentration, but also of the previously described factors that affect to the reactivity of the metals such as the pH or temperature (Tu et al, 2019).…”

Section: Enzymatic Reductive Precipitationmentioning

confidence: 99%

Strategies for nickel and cobalt recovery from mine-impacted water using sulfate-reducing bacteria

Liu¹

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With the increasing world demand and price of valuable metals such as nickel (Ni) and cobalt (Co), it has become more attractive to recover these metals from secondary streams such as mine-impacted water. Biogenic sulfide produced through a biological sulfate reduction process is a promising approach compared with the traditional hydroxide or chemical sulfide precipitation for metal recovery, as it can be produced on site and utilize the substances that already exist in the waste streams. Moreover, it is effective even with low metal concentrations and low pH solutions due to the low solubility products of the metal sulfides. However, the generation of fine particles in the sulfide precipitation process is a challenge and usually results in poor settling, which deteriorates the metal recovery efficiency. Despite this, the compositions of biogenic sulfide solutions from different sources are various depending on the electron donors and the influent characteristics, which may affect the metal precipitation reactions and particle settling properties. Therefore, the effect of using chemical sulfide and four biogenic sulfide-containing solutions for Ni and Co precipitation and settling was evaluated in this thesis. Results showed that phosphate was the main component enhancing particle settling whereas acetate and sulfate exhibited a smaller influence. The Ni and Co recovery were also assessed in a novel single-stage upflow fixed-bed sulfidogenic bioreactor treating metal and sulfate-containing wastewater (0-200 mg/L Ni and 0-10 mg/L Co). Over 99% of Ni and Co precipitated and settled in the bioreactor regardless of the initial metal concentration. Bacterial communities attached on the carrier materials and metal precipitates collected from the bioreactor bottom were also characterized. These findings helped describe the simultaneous treatment of sulfate-and metal-containing wastewater in a sulfidogenic bioreactor and provided information on how to scale up the reactor and to optimize the metal recovery efficiency. Ni and Co often co-exist in ores and the associated mine waste; however, their similar chemical behaviors have made the selective recovery of both metals a challenge. The addition of chemicals and energy in hydrometallurgy are common practices for Ni-Co concentrates that allows selective separation through precipitation, ion exchange and solvent extraction. Although these options are economically feasible for concentrates, the same technologies may not necessarily be attractive for secondary sources with lower, but still significant metal concentrations. Therefore, a new approach to separate Ni and Co using sulfate-reducing bacteria (SRB) based on the different microbial responses to metal stress was proposed in this thesis. Results show that Ni displayed higher solubility (52-99%) than Co (0.6-15.5%) in the presence of SRB and sulfide. This could be partially explained by the higher Ni stress to SRB that induced the production of extracellular proteins that can selectively complex Ni and stabilize it in th...

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Lab-scale evaluation of the microbial bioremediation of Cr(VI): contributions of biosorption, bioreduction, and biomineralization

Xiao

Liu

Zhang

et al. 2021

Environ Sci Pollut Res

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U-phosphate biomineralization induced by Bacillus sp. dw-2 in the presence of organic acids

Cited by 33 publications

References 62 publications

Isolation and Identification of Uranium Tolerant Phosphate-Solubilizing Bacillus spp. and Their Synergistic Strategies to U(VI) Immobilization

Isolation and Identification of Uranium Tolerant Phosphate-Solubilizing Bacillus spp. and Their Synergistic Strategies to U(VI) Immobilization

Strategies for nickel and cobalt recovery from mine-impacted water using sulfate-reducing bacteria

Lab-scale evaluation of the microbial bioremediation of Cr(VI): contributions of biosorption, bioreduction, and biomineralization

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