U-box E3 ubiquitin ligase PUB8 attenuates abscisic acid responses during early seedling growth

Li, Zhipeng; Li, Shaoqin; Jin, Dongjie; Yang, Yongping; Pu, Zhengyan; Han, Xiao; Hu, Yanru; Jiang, Yaling

doi:10.1093/plphys/kiad044

Cited by 8 publications

(5 citation statements)

References 63 publications

(94 reference statements)

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“…Moreover, PUB44 down‐regulates the osmotic stress response by triggering the degradation of CPK4 (Fan et al, 2023). During seedling growth, PUB8 negatively regulates two transcription factors, ABI5 and ABI3, attenuating the ABA response (Li et al, 2023). In this regard, it may be possible that CaPUB24 mediates the degradation of various types of proteins, including cytoplasmic ABA receptors, transcription factors, and protein kinases, involved in ABA signalling.…”

Section: Discussionmentioning

confidence: 99%

Pepper U‐Box E3 ubiquitin ligase 24, CaPUB24, negatively regulates drought stress response

Bae,

Cho,

Choi

et al. 2024

Physiologia Plantarum

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Under stress conditions, plants modulate their internal states and initiate various defence mechanisms to survive. The ubiquitin‐proteasome system is one of the critical modules in these mechanisms, and Plant U‐Box proteins play an important role in this process as E3 ubiquitin ligases. Here, we isolated the Plant U‐box 24 gene CaPUB24 (Capsicum annuum Plant U‐Box 24) from pepper and characterized its functions in response to drought stress. We found that, compared to the other CaPUBs in the same group, the expression of CaPUB24 was significantly induced by drought stress. We also found that CaPUB24 was localized to the nucleus and cytoplasm and had E3 ubiquitin ligase activity. To investigate the biological role of CaPUB24 in response to drought stress further, we generated CaPUB24‐silenced pepper plants and CaPUB24‐overexpressing Arabidopsis transgenic plants. CaPUB24‐silenced pepper plants exhibited enhanced drought tolerance compared to the control plants due to reduced transpirational water loss and increased abscisic acid (ABA) sensitivity. In contrast, CaPUB24‐overexpressing Arabidopsis transgenic plants exhibited reduced drought tolerance and ABA‐insensitive phenotypes. Our findings suggest that CaPUB24 negatively modulates drought stress response in an ABA‐dependent manner.

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Section: Discussionmentioning

confidence: 99%

Pepper U‐Box E3 ubiquitin ligase 24, CaPUB24, negatively regulates drought stress response

Bae,

Cho,

Choi

et al. 2024

Physiologia Plantarum

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“…Total RNA was extracted by using Trizol reagent (Invitrogen). The RT-qPCR analysis was conducted as described (He et al, 2023; Li et al, 2023). In brief, 1.0 μg DNase-treated RNA was reverse transcribed in a reaction volume of 20 μl containing oligo-(dT)19 primer and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

“…The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 and injected into tobacco ( N. benthamiana ) leaves. As described previously (He et al, 2023; Li et al, 2023), YFP and 4,6-diamidino-2-phenylindole (DAPI) fluorescence of infected leaves were observed under a confocal laser scanning microscope (Olympus) after 48 h of co-expression. Primers used for cloning are listed in Supplemental Data Set 1.…”

Section: Methodsmentioning

confidence: 99%

“…To identify structural domains essential for protein interactions, several truncated NPR1 and TGA3 were cloned into pGBKT7 and truncated ICE1 into pGADT7. The yeast two hybrid (Y2H) assays were performed as described previously (Li et al, 2023). The vector pairs were co-transformed into the yeast strain AH109, protein interactions were indicated by the ability of cells to grow on dropout medium lacking Leu, Trp, His, and Ade for 2 days after plating.…”

Section: Methodsmentioning

confidence: 99%

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Inducer of CBF Expression 1 (ICE1) Promotes Cold-enhanced Immunity by Directly Activating Salicylic Acid Signaling

Li,

He,

Yang

et al. 2023

Preprint

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Cold stress affects plant immune responses, and this process may involve the salicylic acid (SA) signaling pathway. However, the underlying mechanism by which low temperature signals coordinate with SA signaling to regulate plant immunity remains poorly characterized. Here, we found that low temperatures enhanced the disease resistance of Arabidopsis against Pseudomonas syringae pv. tomato (Pst) DC3000. This process required Inducer of CBF expression 1 (ICE1), the core transcription factor in cold-signal cascades. ICE1 physically interacted with Non-expresser of PR genes 1 (NPR1), the master regulator of the SA signaling pathway. Enrichment of ICE1 on the PR1 promoter and its ability to transcriptionally activate PR1 were enhanced by NPR1. Further analyses revealed that cold stress signals cooperate with SA signals to facilitate plant immunity against pathogen attack in an ICE1-dependent manner. Cold treatment promoted interactions of NPR1 and TGA3 with ICE1, and increased the ability of the ICE1-TGA3 complex to transcriptionally activate PR1. Together, our results characterize a previously unrecognized role of ICE1 as an indispensable regulatory node linking low temperature activated- and SA-regulated immunity. Discovery of a crucial role of ICE1 in coordinating multiple signals associated with immunity broadens our understanding of plant-pathogen interactions.

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“…Furthermore, ubiquitination-mediated protein degradation is an important process in ABA signal transduction. [ 20 , 21 ]. In regulating the expression of stress-responsive genes, transcription factors, post-transcriptional modification and secondary metabolism play a vital role in responding to abiotic stress [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Mining of CULLIN E3 Ubiquitin Ligase Genes from Uncaria rhynchophylla

Shao,

Mu,

Zhou

et al. 2024

Plants

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CULLIN (CUL) protein is a subtype of E3 ubiquitin ligase that is involved in a variety of biological processes and responses to stress in plants. In Uncaria rhynchophylla, the CUL gene family has not been identified and its role in plant development, stress response and secondary metabolite synthesis has not been studied. In this study, 12 UrCUL gene members all contained the typical N-terminal domain and C-terminal domain identified from the U. rhynchophylla genome and were classified into four subfamilies based on the phylogenetic relationship with CULs in Arabidopsis thaliana. They were unevenly distributed on eight chromosomes but had a similar structural composition in the same subfamily, indicating that they were relatively conserved and potentially had similar gene functions. An interspecific and intraspecific collinearity analysis showed that fragment duplication played an important role in the evolution of the CUL gene family. The analysis of the cis-acting elements suggests that the UrCULs may play an important role in various biological processes, including the abscisic acid (ABA) response. To investigate this hypothesis, we treated the roots of U. rhynchophylla tissue-cultured seedlings with ABA. The expression pattern analysis showed that all the UrCUL genes were widely expressed in roots with various expression patterns. The co-expression association analysis of the UrCULs and key enzyme genes in the terpenoid indole alkaloid (TIA) synthesis pathway revealed the complex expression patterns of 12 UrCUL genes and some key TIA enzyme genes, especially UrCUL1, UrCUL1-likeA, UrCUL2-likeA and UrCUL2-likeB, which might be involved in the biosynthesis of TIAs. The results showed that the UrCULs were involved in the response to ABA hormones, providing important information for elucidating the function of UrCULs in U. rhynchophylla. The mining of UrCULs in the whole genome of U. rhynchophylla provided new information for understanding the CUL gene and its function in plant secondary metabolites, growth and development.

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U-box E3 ubiquitin ligase PUB8 attenuates abscisic acid responses during early seedling growth

Cited by 8 publications

References 63 publications

Pepper U‐Box E3 ubiquitin ligase 24, CaPUB24, negatively regulates drought stress response

Pepper U‐Box E3 ubiquitin ligase 24, CaPUB24, negatively regulates drought stress response

Inducer of CBF Expression 1 (ICE1) Promotes Cold-enhanced Immunity by Directly Activating Salicylic Acid Signaling

Genome-Wide Mining of CULLIN E3 Ubiquitin Ligase Genes from Uncaria rhynchophylla

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