Tyrosine protein phosphorylation in murine B lymphocytes by stimulation with lipopolysaccharide fromPorphyromonas gingivalis

Abstract

The molecular effect of lipopolysaccharides (LPS) from porphyromonas gingivalis as well as Escherichia coli on the tyrosine protein phosphorylation in the splenic B lymphocytes from LPS-responsive C3H/HeN and LPS-hyporesponsive C3H/HeJ mice was examined. P. gingivalis LPS induced tyrosine phosphorylation of selected membrane proteins that included the phosphoproteins with apparent molecular masses of 24.8 kDa and 26.0 kDa (p24.8 and p26.) in the B lymphocytes from both strains of mice, while E. coli LPS induced p24.8 and p26.0 in C3H/HeN B Lymphocytes only. These findings suggest that through the same tyrosine phosphorylation pathway as observed in C3H/HeN B lymphocytes, P. gingivalis LPS induced the activation of C3H/HeJ B lymphocytes in which a trigger signal by E. coli LPS could not be transduced to initiate tyrosine protein phosphorylation.

Antigenic characterisation of a novel Streptococcus anginosus antigen that induces nitric oxide synthesis by murine peritoneal exudate cells

“…These antibodies were used in an immunodiffusion test against SAA and RRA in an agar 1% gel with 0.01 M sodium phosphate buffer (pH 7.2). For further antigenic characterisation of SAA, a Western blot analysis was performed with the antibodies, as described previously [24,25]. Brie¯y, SAA (2 ìg of protein) was separated by SDS-PAGE (12.5% gel).…”

Induction of Experimental Periodontitis in Mice With Porphyromonas gingivalis‐ Adhered Ligatures

“…,26 For the Western blot analysis, bacterial samples were suspended in SDS sample buffer and then boiled for 5 minutes. The samples were separated by electrophoresis on SDS-polyacrylamide…”

Bioactive mechanism of Porphyromonas gingivalis lipid A

“…It was demonstrated that Bacteroides lipopolysaccharide and lipid A, derived from different bacterial species that are similar to Bacteroides fragilis , such as P. gingivalis (formerly Bacteroides gingivalis ), Prevotella intermedia (formerly Bacteroides intermedius ), Prevotella oralis (formerly Bacteroides oralis ), Prevotella loescheii (formerly Bacteroides loescheii ) and Prevotella corporis (formerly Bacteroides corporis ), activate B lymphocytes and macrophages of C3H/HeJ mice (6, 11, 14, 15, 17, 20–23, 25–27, 49, 52, 53). We also reported that P. gingivalis lipopolysaccharide and lipid A have mitogenic effects and induce cytokine production by B lymphocytes from C3H/HeJ mice (12, 40, 41, 45).…”