Tyrosine hydroxylase immunoreactivity in the developing visual pathway of the zebrafish

Arenzana, Francisco Javier; Arévalo, R.; Sánchez-González, Rosario; Clemente, Diego; Aijón, José; Porteros, A.

doi:10.1007/s00429-006-0084-2

Cited by 29 publications

(22 citation statements)

References 53 publications

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“…2). th -expressing cells correspond to the dopaminergic amacrine cells in the retina [76,77], which are interneurons that play a role in determining the sensitivity of other retinal neurons during light adaptation in other species [78–80]. Thus Chd5 morphants exhibit a highly penetrant defect in differentiation of at least one type of neuron, consistent with the recently established role for CHD5 in neurogenesis in mammalian systems [13].…”

Section: Resultssupporting

confidence: 58%

The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio

Bishop

Tyler

et al. 2015

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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The chromatin remodeler CHD5 plays a critical role in tumor suppression and neurogenesis in mammals. CHD5 contributes to gene expression during neurogenesis, but there is still much to learn regarding how this class of remodelers contributes to differentiation and development. CHD5 remodelers are vertebrate-specific, raising the prospect that CHD5 plays one or more conserved roles in this phylum. Expression of chd5 in adult fish closely mirrors expression of CHD5 in adult mammals. Knockdown of Chd5 during embryogenesis suggests new roles for CHD5 remodelers based on resulting defects in craniofacial development including reduced head and eye size as well as reduced cartilage formation in the head. In addition, knockdown of Chd5 results in altered expression of neural markers in the developing brain and eye as well as a profound defect in differentiation of dopaminergic amacrine cells. Recombinant zebrafish Chd5 protein exhibits nucleosome remodeling activity in vitro, suggesting that it is the loss of this activity that contributes to the observed phenotypes. Our studies indicate that zebrafish is an appropriate model for functional characterization of CHD5 remodelers in vertebrates and highlight the potential of this model for generating novel insights into the role of this vital class of remodelers.

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Section: Resultssupporting

confidence: 58%

The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio

Bishop

Tyler

et al. 2015

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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“…-Anti-tyrosine hydroxylase (TH) antibody (Incstar, Stillwater, MN, USA) [31], at a 1∶1,000 concentration. Secondary antibody labeled with Cy2 (green).…”

Section: Methodsmentioning

confidence: 99%

Optimization and In Vivo Toxicity Evaluation of G4.5 Pamam Dendrimer-Risperidone Complexes

et al. 2014

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Risperidone is an approved antipsychotic drug belonging to the chemical class of benzisoxazole. This drug has low solubility in aqueous medium and poor bioavailability due to extensive first-pass metabolism and high protein binding (>90%). Since new strategies to improve efficient treatments are needed, we studied the efficiency of anionic G4.5 PAMAM dendrimers as nanocarriers for this therapeutic drug. To this end, we explored dendrimer-risperidone complexation dependence on solvent concentration, pH and molar relationship. The best dendrimer-risperidone incorporation (46 risperidone molecules per dendrimer) was achieved with a mixture of chloroform:methanol 50∶50 v/v solution pH 3. In addition, to explore the possible effects of this complex, in vivo studies were carried out in the zebrafish model. Changes in the development of dopaminergic neurons and motoneurons were studied using tyrosine hydroxylase and calretinin, respectively. Physiological changes were studied through histological sections stained with hematoxylin-eosin to observe possible morphological brain changes. The most significant changes were observed when larvae were treated with free risperidone, and no changes were observed when larvae were treated with the complex.

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“…Interestingly, peripheral clocks in this species can be entrained by direct exposure to LD cycles [16]. However, zebrafish also possess central pacemakers: a structural counterpart of the SCN and a photosensitive pineal complex where nighttime synthesis of the hormone melatonin is directed by an endogenous clock [15,17–20]. …”

Section: Introductionmentioning

confidence: 99%

Glucocorticoids Play a Key Role in Circadian Cell Cycle Rhythms

et al. 2007

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Clock output pathways play a pivotal role by relaying timing information from the circadian clock to a diversity of physiological systems. Both cell-autonomous and systemic mechanisms have been implicated as clock outputs; however, the relative importance and interplay between these mechanisms are poorly understood. The cell cycle represents a highly conserved regulatory target of the circadian timing system. Previously, we have demonstrated that in zebrafish, the circadian clock has the capacity to generate daily rhythms of S phase by a cell-autonomous mechanism in vitro. Here, by studying a panel of zebrafish mutants, we reveal that the pituitary–adrenal axis also plays an essential role in establishing these rhythms in the whole animal. Mutants with a reduction or a complete absence of corticotrope pituitary cells show attenuated cell-proliferation rhythms, whereas expression of circadian clock genes is not affected. We show that the corticotrope deficiency is associated with reduced cortisol levels, implicating glucocorticoids as a component of a systemic signaling pathway required for circadian cell cycle rhythmicity. Strikingly, high-amplitude rhythms can be rescued by exposing mutant larvae to a tonic concentration of a glucocorticoid agonist. Our work suggests that cell-autonomous clock mechanisms are not sufficient to establish circadian cell cycle rhythms at the whole-animal level. Instead, they act in concert with a systemic signaling environment of which glucocorticoids are an essential part.

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Tyrosine hydroxylase immunoreactivity in the developing visual pathway of the zebrafish

Cited by 29 publications

References 53 publications

The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio

The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio

Optimization and In Vivo Toxicity Evaluation of G4.5 Pamam Dendrimer-Risperidone Complexes

Glucocorticoids Play a Key Role in Circadian Cell Cycle Rhythms

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