Tyrosine Hydroxylase, a Potential Target for the RNAi-Mediated Management of Diamondback Moth (Lepidoptera: Plutellidae)

Ellango, R.; Asokan, R.; Chandra, G. Sharath; Kumar, N. K. Krishna; Mahmood, Riaz; Ramamurthy, V. V.

doi:10.1653/024.101.0102

Cited by 9 publications

(7 citation statements)

References 28 publications

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“…It was reported that the dietary TH double-stranded RNA led to reduced target gene transcript levels and larval feeding levels and caused larval mortality (Ellango et al, 2018). However, in our study we utilized the CRISPR/Cas9 system to mediate the loss of function of AiTH.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9‐mediated Tyrosine hydroxylase knockout resulting in larval lethality in Agrotis ipsilon

et al. 2018

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Tyrosine hydroxylase (TH) is involved in insect melanin and the catecholamine biosynthesis pathway. TH as an enzyme catalyzing the conversion of tyrosine to 3,4‐dihydroxyphenylalanine is the first step reaction in the pathway. Although TH has been proven to affect the pigmentation of the epidermis and development in many insects, there is no report about physiological function of the TH gene in Agrotis ipsilon. Here we cloned the TH gene from A. ipsilon. Semi‐quantitative real‐time polymerase chain reaction (PCR) analysis showed that AiTH was expressed at all development stages. Moreover, its high expression levels in the head and epidermis suggest that it is mainly related to pigment deposition and insect development. Then, we used the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 system to target the AiTH gene: deletion events were detected at the target sites. Compared with the control group, a few mutants with the phenomenon of narrowing in the egg shell and embryos can develop but cannot hatch; the other hatched embryos were seriously dehydrated after hatching and died within the first day. Quantitative real‐time PCR analysis revealed that TH was down‐regulated in AiTH mutants. Here, our work demonstrated that AiTH plays an important role in growth and development of newly hatched larvae; meanwhile, it would be a promising target to explore a control strategy for A. ipsilon.

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Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9‐mediated Tyrosine hydroxylase knockout resulting in larval lethality in Agrotis ipsilon

et al. 2018

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“…2 ). Some studies have confirmed that DOPA decarboxylase has been conducted to clarify the participation of biogenic amines in ovulation and egg laying; in the study on dopa decarboxylase in Drosophila sechellia (Diptera: Drosophilidae) and Plutella xylostella (Lepidoptera: Plutellidae), it was found that DOPA decarboxylase can affect the survival rate of larvae and the reproductive activity of female adults, and the silence of Dopa decarboxylase genes will reduce the number of individuals in the next generation, which indicates that DOPA decarboxylase plays an important role in the oviposition of female adults ( Ellango et al 2018 , Lanno et al 2019 ).…”

Section: Genes Related To Oviposition and Hatchingmentioning

confidence: 99%

Research Progress on Oviposition-Related Genes in Insects

Wang

Zheng

et al. 2020

Journal of Insect Science

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Oviposition-related genes have remained a consistent focus of insect molecular biology. Previous research has gradually clarified our mechanistic understanding of oviposition-related genes, including those related to oviposition-gland-related genes, oogenesis-related genes, oviposition-site-selection-related genes, and genes related to ovulation and hatching. Moreover, some of this research has revealed how the expression of single oviposition-related genes affects the expression of related genes, and more importantly, how individual node genes function to link the expression of upstream and downstream genes. However, the research to date is not sufficient to completely explain the overall interactions among the genes of the insect oviposition system. Through a literature review of a large number of studies, this review provides references for future research on oviposition-related genes in insects and the use of RNAi or CRISPR/Cas9 technology to verify the functions of oviposition-related genes and to prevent and control harmful insects.

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“…Therefore, Th functions as a key modulator in cuticle sclerotization and innate immunity, and its defect is lethal. Although TH is crucial for insect development and survival, TH studies have only focused on holometabolous insects, such as Dipteran flys, Coleopteran beetles, and Lepidopteran species . Until now, little information is available for hemimetabolous insects.…”

Section: Introductionmentioning

confidence: 99%

RNA interference of tyrosine hydroxylase caused rapid mortality by impairing cuticle formation in Nilaparvata lugens (Hemiptera: Delphacidae)

Liu

Yang

Wang

et al. 2020

Pest Management Science

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BACKGROUND: The application of RNA interference (RNAi) technique in controlling agricultural insect pests has been receiving much attention since the discovery of RNAi. The brown planthopper (BPH) Nilaparvata lugens, a notorious pest of rice, has evolved a high level of resistance to many kinds of insecticides. Tyrosine hydroxylase (Th) is an indispensable survival gene in holometabolous insects, playing key roles in cuticle tanning and immunity. In this study, we investigated whether Th could be used as a potential target in controlling N. lugens.RESULTS: Here, we demonstrated that NlTh had a periodical expression pattern during molting with the highest level observed in epidermis. Dysfunction of NlTH by dsNlTh microinjection or 3-IT feeding similarly caused rapid death of N. lugens. Compared with dsGFP control BPHs, dsNlTh injected BPHs (i) had cuticle pigmentation and sclerotizaton defects; (ii) had less endocuticle lamella in tergum integument; (iii) showed higher mortality during the molting process as a result of defective cuticle shedding; (iv) showed feeding disorders indicated by a low number of probe wound dots on rice; (v) had more vulnerable cuticle.CONCLUSION: This study demonstrated that TH orthologues play a conservative and crucial role for exocuticle tanning in both holometabolous and hemimetabolous insects, and NlTh could be targeted for RNAi-mediated BPH control. The rapid lethal phenotype of NlTH dysfunction BPHs partly induced by cuticle formation defects.

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Tyrosine Hydroxylase, a Potential Target for the RNAi-Mediated Management of Diamondback Moth (Lepidoptera: Plutellidae)

Cited by 9 publications

References 28 publications

CRISPR/Cas9‐mediated Tyrosine hydroxylase knockout resulting in larval lethality in Agrotis ipsilon

CRISPR/Cas9‐mediated Tyrosine hydroxylase knockout resulting in larval lethality in Agrotis ipsilon

Research Progress on Oviposition-Related Genes in Insects

RNA interference of tyrosine hydroxylase caused rapid mortality by impairing cuticle formation in Nilaparvata lugens (Hemiptera: Delphacidae)

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