Tyrosinase Inhibition from Green Tea (Camellia sinensis (L.) Kuntze) gel

Abstract

Green tea (Camellia sinensis (L.) Kuntze) leaf has polyphenol substance that able to inhibit tyrosinase enzyme. Tyrosinase enzyme is one of the essential components that can be initiated melanin formation on the skin (melanogenesis). The natural inhibitory enzyme can be utilized in cosmetics and medicinal industries as depigmentation agent. Green tea leaf was formulated in the dosage form of a gel with carbomer 934 as a gelling agent. This study aimed to determine the optimum concentration of carbomer 934 on green tea leaf extract gel that has an inhibitory tyrosinase activity. This research used three carbomer 934 concentration, there was 0.5%, 0.75%, and 1% respectively. Which every formula was evaluated during six weeks involve organoleptic, homogeneity, viscosity, pH, centrifuge, and freeze-thaw test (during six cycles). The optimum formula was evaluated tyrosinase activity used a spectrophotometer-vis. The results showed that the great concentration of carbomer 934 was contained on the first formula, which has not phase separation and 49.62 ppm of IC 50 value. This research showed that green tea leaf extract gel with 0,5% carbomer 934 prevented tyrosinase activity.

Potensi Madu dan Ekstrak Ampas Teh Sebagai Penghambat Tirosinase Untuk Bahan Aktif Kosmetika Pemutih

“…Hal ini beralasan karena Indonesia adalah produsen teh terbesar ke-5 di dunia setelah Sri Lanka, Kenya, India dan China, dengan tingkat produksi mencapai 154.598 ton/tahun [6]. Padahal, pada ekstrak daun teh hijau (Camellia sinensis (L.) Kuntze) terdapat senyawa polifenol yang berpotensi menghambat tirosinase pada konsentrasi 0,5% [7] dan teh kombucha dari buah anggur berpotensi sebagai anti penuaan [8].…”

“…Melanogenesis adalah proses dimana pigmen melanin diproduksi (Gambar 2). Enzim penghambat alami dapat dimanfaatkan dalam industri kosmetik dan obat-obatan sebagai agen depigmentasi [7].…”