Tyrosinase biosensor response as a function of physical properties of organic solvents

Campanella, Luigí; Fortuney, A.; Sammartino, Maria Pia; Tomassetti, M.

doi:10.1016/0039-9140(93)e0062-i

Cited by 42 publications

(7 citation statements)

References 12 publications

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“…Tyrosinase, a copper containing tetrameric enzyme with a molecular weight of 128 Á/133 kDa (Kertesz and Zito, 1965), catalyses the 4e ( oxidation of phenolics by dioxygen from the air (see Solomon et al, 2001). In dry organic media enzymatic activity of tyrosinase is drastically depressed due to enzyme inactivation by organic solvent: for retention of the catalytic activity a minimal water content is obligatory (Campanella et al ., 1994;Yang and Robb, 1993). Studies of tyrosinase in micellar systems resulted in determination of the optimum tyrosinase activity at a hydration degree 20 Á/25 (Rodakiewicz-Nowak et al, 2002;Rojo et al ., 2001;Sanchez-Ferrer et al ., 1995;Yang and Robb, 1993).…”

Section: Introductionmentioning

confidence: 99%

Entrapping of Tyrosinase in a System of Reverse Micelles

Shipovskov

Levashov

2004

Biocatalysis and Biotransformation

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The enzymatic activity of tyrosinase was studied both in aqueous and organic media. In the latter case tyrosinase was entrapped in a system of reverse micelles of Aerosol OT in octane. At hydration degree 25, when the inner cavity of the reverse micelles was comparable with the size of a tetrameric tyrosinase form known for aqueous solutions, an optimum level of catalytic activity was observed. Another peak of catalytic activity of tyrosinase was observed at hydration degree 12, when the size of the inner cavity of the reverse micelles was consistent with a monomeric form of tyrosinase. Thus, the system of reverse micelles can be exploited as a medium for the investigation of the monomeric form of tyrosinase, which is unstable in aqueous solution.

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Section: Introductionmentioning

confidence: 99%

Entrapping of Tyrosinase in a System of Reverse Micelles

Shipovskov

Levashov

2004

Biocatalysis and Biotransformation

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“…Behaviour of the tyrosinase enzyme electrode has been investigated under different experimental conditions [30][31][32][33]. Several authors [34][35][36][37][38][39] have tested the performance of the tyrosinase electrode in different organic solvents and the effect of different additives has also been investigated [40,41]. Organic phase enzyme electrodes constitute a new class of biosensor applicable to the analysis of substrates or matrices insoluble or scarcely soluble in aqueous media.…”

Section: Enzymatic (Catalytic)mentioning

confidence: 99%

(Bio)Sensor Approach in the Evaluation of Polyphenols in Vegetal Matrices

Bergonzi¹,

Minunni

Bilia³

2008

Natural Product Communications

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Polyphenols are compounds widely distributed in the plant kingdom and have attracted much attention, because of their health benefits and important properties such as radical scavenging, metal chelating agents, inhibitors of lipoprotein oxidation, anti-inflammatory and anti-allergic activities. Due to their important role in the diet and in therapy, it is important to estimate their content in the different matrices of interest. Besides classical analytical methods, new emerging technologies have also appeared in the last decade aiming for simple and eventually cheap detection of polyphenols. This review focused on the recent applications of biosensing-based technologies for polyphenol estimation in vegetal matrices, using different transduction principles. These analytical tools are generally fast, giving responses in the order of a few seconds/minutes, and also very sensitive and generally selective (mainly depending on the enzyme used). Direct measurements in most of the investigated matrices were possible, both in aqueous and organic phases.

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“…Methods for rapid determination of ethanol content in green fuels and biofuels are therefore increasingly in demand. Our research group has long experience in the development of sensors, biosensors and immunosensors of different types [ 2 , 6 , 21 , 22 ], but in particular in fabricating OPEEs [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ] and Organic Phase Immuno Electrodes (OPIEs) [ 30 , 31 ]. One of the most recent OPEEs we developed for the determination of ethanol in organic solvents [ 32 ] is based on the catalase enzyme.…”

Section: Introductionmentioning

confidence: 99%

Bioethanol in Biofuels Checked by an Amperometric Organic Phase Enzyme Electrode (OPEE) Working in “Substrate Antagonism” Format

Tomassetti

Capesciotti

Angeloni

et al. 2016

Sensors

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The bioethanol content of two samples of biofuels was determined directly, after simple dilution in decane, by means of an amperometric catalase enzyme biosensor working in the organic phase, based on substrate antagonisms format. The results were good from the point of view of accuracy, and satisfactory for what concerns the recovery test by the standard addition method. Limit of detection (LOD) was on the order of 2.5 × 10−5 M.

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Tyrosinase biosensor response as a function of physical properties of organic solvents

Cited by 42 publications

References 12 publications

Entrapping of Tyrosinase in a System of Reverse Micelles

Entrapping of Tyrosinase in a System of Reverse Micelles

(Bio)Sensor Approach in the Evaluation of Polyphenols in Vegetal Matrices

Bioethanol in Biofuels Checked by an Amperometric Organic Phase Enzyme Electrode (OPEE) Working in “Substrate Antagonism” Format

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