Tyrosinase Biosensor for Phenol Monitoring in Water

Abstract

Phenol and its

Techno-economic design requirements for integrating biosensors in environmental monitoring of aquatic ecosystems

“…The results presented herein for the metal-supported enzyme biosensor built on tyrosinase to quantitate phenol based on the enzyme-analyte initial interaction and not the commonly adopted monitoring of the redox cascade reactions (Siontorou and Georgopoulos, 2015); such an approach has not been proposed in literature thus far and offers many advantages for environmental monitoring. The sensitivity demonstrated was higher than that of other tyrosinase-based biosensor systems, comparable to chromatography and immunoassays, whereas the pg/ml detection limit achieved was quite lower, enabling environmental screening of phenol at concentrations within and above the environmental limits.…”

“…Optimized sensors have been developed on complex multilayer constructs of graphene-Au nanoparticle-platforms with chitosan-bound tyrosinase with a detection limit of 4.67 ng/ml (Fartas et al, 2017). To avoid fouling and electrochemical interference, the tyrosinase-phenol primary interaction (i.e., at the oxy-state) has been investigated herein for the development of a metal-supported lipid-membrane phenols biosensor (Siontorou and Georgopoulos, 2015). To our knowledge, such an approach has not been proposed to literature so far.…”