Typoselectivity of CrudeGeobacillussp. T1 Lipase Fused with a Cellulose‐Binding Domain and Its Use in the Synthesis of Structured Lipids

Abstract

Typoselectivity of crude CBD-T1 lipase (Geobacillus sp. T1 lipase fused with a cellulose binding domain) was investigated. Multi-competitive reaction mixtures including a set of n-chain fatty acids (C8:0, C10:0, C12:0, C14:0, C18:1 n-9, C18:2 n-6 and C18:3 n-3) and tripalmitin-enriched triacylglycerols were studied in hexane. The crude CBD-T1 lipase discriminated strongly against C18:1 n-9 [competitive factor (a) = 0.23] and showed the highest preference for C8:0 (a = 1). Utilizing the catalytic properties of crude CBD-T1 lipase, acidolysis of soybean oil with C8:0 was selected as a model reaction to investigate the ability of the lipase to produce MLMtype (medium-long-medium) structured lipids. Several reaction parameters (added water amount, reaction temperature, substrate molar ratio and reaction time) examined for incorporating C8:0 into soybean oil, the optimum conditions were: 1:3 (soybean oil/C8:0) of molar ratio, 3 mL of hexane, 50°C of temperature, 48 h of reaction time, 20 % of crude CBD-T1 lipase (w/w total substrates), and 7.5 % of water (w/w enzyme). Under these conditions, the incorporation of C8:0 was 29.6 mol%. The results suggest that crude CBD-T1 lipase, which showed different fatty acid specificity profiles, is a potential biocatalyst for the modification of fats and oils.

Deep Eutectic Solvents Enable the Enhanced Production of n‐3 PUFA‐Enriched Triacylglycerols

“…Thin‐layer chromatography was applied to separate TAG from other compositions . According to ISO 5509:2000 (E), TAG and n‐3 PUFA were separately methylated to fatty acid methyl esters .…”

Production and immobilization of lipase PCL and its application in synthesis of α-linolenic acid-rich diacylglycerol

“…Then, the scraped DAG, MAG, and the unreacted substrate (FA) were separately methylated to FAME according to the method described by Wang et al (). Finally, the analysis of the FA composition was performed using GC (Agilent 7890A) equipped with a capillary column CP‐Sil 88 (60m × 0.25mm × 0.2 μm) according to the method of Qin, Huang, Lan, Wang, and Yang ().…”

Karakteristik Lipid Terstruktur Hasil Transesterifikasi Enzimatik Antara Minyak Ikan Dan Minyak Kelapa Mur

“…Penelitian mengenai sintesis TAG tipe MLM telah banyak dilakukan dengan menggunakan berbagai jenis substrat antara lain minyak alpukat dan asam kaprilat (Caballero et al 2014), substrat trikaprilat dan trilinolenat (Bai et al 2013), minyak zaitun dan asam kaprilat (Nunes et al 2011), minyak kanola dan asam kaprilat (Wang et al 2012;Savaghebi et al 2012) dan minyak kedelai dan asam kaprilat (Qin et al 2014), minyak jagung dan asam kaprilat (Ozturk et al 2010), palm stearin dengan minyak ikan lemuru (Hastuti et al 2003), minyak ikan dengan asam kaprilat (Sousa et al 2014), minyak ikan dan asam laurat (Subroto et al 2008dan Mamuaja 2015. Tujuan penelitian ini adalah menetapkan lama proses reaksi transesterifikasi enzimatik terbaik untuk menghasilkan lipid terstruktur tipe MLM (medium-long-medium) dan menentukan karakteristik kimia dan fisik lipid terstruktur.…”