Typing of Pseudomonas aeruginosa strains in Norwegian cystic fibrosis patients

Fluge, G; Ojeniyi, B.; Høiby, Niels; Digranes, Asbjørn; Ciofu, Oana; Hunstad, E.J.; Haanaes, Ole Christen; Storrøsten, Olav Trond

doi:10.1046/j.1469-0691.2001.00247.x

Cited by 42 publications

(27 citation statements)

References 32 publications

(40 reference statements)

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“…However, multiple reports from the UK, Australia and Norway suggest that cross-infection between unrelated patients occurs much more frequently than previously recognised [3][4][5][6][7]10]. In keeping with the current findings, the studies from Liverpool, Melbourne, Brisbane and Bergen all found that the majority of P. aeruginosa-infected patients attending their centres harboured clonal isolates.…”

Section: Discussionsupporting

confidence: 87%

“…Typically, most patients are infected with a single clonal genotype (e.g. Liverpool or Md1) while the remainder are infected with a number of other genotypes [3,4,6,10]. The findings of the Brisbane group in particular very closely mirror the current findings, as 59% of patients were infected with a clonal genotype (39% with the same genotype (pulsotype 2) and 20% with multiple other small clusters) [4].…”

Section: Discussionsupporting

confidence: 80%

“…antibiotic use or number of hospitalisations. A similarly benign P. aeruginosa genotype has been identified in Norway [10]. This finding is important since it suggests that, at least for these P. aeruginosa genotypes, the bacterial factors associated with the ability to more successfully establish persistent infection may not necessarily be the same as those associated with virulence and severe disease, as had originally been feared [3,11].…”

Section: Discussionmentioning

confidence: 90%

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Factors associated with infection by Pseudomonas aeruginosa in adult cystic fibrosis

Chambers¹

2005

European Respiratory Journal

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Cross-infection with Pseudomonas aeruginosa is an emerging issue in the care of patients with cystic fibrosis (CF). This study sought to determine the extent of, and patient factors associated with, cross-infection in a tertiary referral adult CF centre.P. aeruginosa isolates were genotyped into two groups between November 2001 and February 2003, using pulsed-field gel electrophoresis after DNA digestion by the SpeI endonuclease, and identified as clustered if there was .80% homology in the macrorestriction profiles. Patient factors and measures of disease severity were identified a priori.In total, 157 out of 227 patients had a P. aeruginosa isolate genotyped. Of these, 94 patients (60%) were infected with clustered genotypes and 47 (30%) were infected with the newly described ''Midlands 1'' (Md1) genotype. A further 18 patients were infected with the previously identified ''Liverpool'' genotype and two with the ''Manchester'' genotype. Logistic regression analysis revealed that the predominant predictor of infection with Md1 was age at the time of referral to the centre, suggesting that infection may have occurred prior to referral in some patients. Md1 demonstrated a relatively benign anti-biogram and did not appear to be associated with more severe disease.In conclusion, the present study provides further evidence of the emerging importance of Pseudomonas aeruginosa cross-infection in cystic fibrosis.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 90%

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Factors associated with infection by Pseudomonas aeruginosa in adult cystic fibrosis

Chambers¹

2005

European Respiratory Journal

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“…Among the 19 Norwegian patients, one dominant clone was found, clone N (13 patients). The presence of this dominant clone among Norwegian CF patients was published previously (Fluge et al, 2001). Unique clones among P. aeruginosa isolates from Denmark (Ud), Sweden (Us) and Norway (Un) were found in the rest of the patients.…”

Section: Clonal Distribution Of the Mucoid And Non-mucoid Isolatesmentioning

confidence: 54%

Investigation of the algT operon sequence in mucoid and non-mucoid Pseudomonas aeruginosa isolates from 115 Scandinavian patients with cystic fibrosis and in 88 in vitro non-mucoid revertants

et al. 2008

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Investigation of the algT operon sequence in mucoid and non-mucoid Pseudomonas aeruginosa isolates from 115 Scandinavian patients with cystic fibrosis and in 88 in vitro non-mucoid revertants Pseudomonas aeruginosa is the dominant pathogen causing chronic lung infections in patients with cystic fibrosis (CF). After an initial phase characterized by intermittent colonizations, a chronic infection is established upon conversion of P. aeruginosa from the non-mucoid to the mucoid, alginate-overproducing phenotype. During the chronic infection the isolation of both mucoid and non-mucoid isolates in CF sputum samples is very common. The purpose of the present study was to establish, by sequence analysis, the types of mutations present in the algTmucABD operon in a large number of mucoid and non-mucoid P. aeruginosa isolates from Scandinavian CF patients and in in vitro-derived non-mucoid revertants. Mucoid (83) and non-mucoid isolates (103) from 91 Scandinavian patients with chronic P. aeruginosa infection and 24 non-mucoid isolates from intermittently colonized CF patients were investigated. In addition, 88 spontaneous non-mucoid revertants obtained in vitro from nine mucoid CF isolates were also included in the study. Mutations in mucA were found in 92 % of the mucoid and in up to 70 % of the non-mucoid isolates from chronically infected patients, indicating that the majority of non-mucoid isolates are revertants. None of the non-mucoid isolates from intermittently colonized CF patients harboured mucA mutations. Although algT has been considered an important gene for secondary-site mutations responsible for reversion to non-mucoidy, only 30 % of the mucA-mutated non-mucoid CF isolates had mutations in algT. In contrast, 83 % of the in vitro-derived spontaneous non-mucoid revertants had mutations in algT, showing that in the CF lung there is a selection for non-mucoid revertants with secondary-site mutations in genes other than algT. In addition, we report, to our knowledge for the first time, loss-of-function mutations in the negative regulators mucB and mucD in CF clinical isolates. In some of the CF isolates these mutations are associated with moderate alginate production. In conclusion, most non-mucoid isolates from chronically infected CF patients are revertants and the mechanism of revertance is algT-independent in the CF lung.

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“…siblings 63 or patients who visited holiday camps together 64 . This view has been disputed by reports of P. aeruginosa clones that are shared between patients, suggesting an important role for patient to patient transmission [65][66][67][68][69] as these clones are not isolated from the environment. Strong evidence for patient to patient spread was reported from the cf center in Liverpool, where molecular fingerprinting techniques identified an outbreak of a -lactam resistant P. aeruginosa strain (referred to as the Liverpool Epidemic Strain, les) 65 .…”

mentioning

confidence: 99%

Challenging and emerging pathogens in cystic fibrosis

Vrankrijker

Wolfs

Ent

2010

Paediatric Respiratory Reviews

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Typing of Pseudomonas aeruginosa strains in Norwegian cystic fibrosis patients

Abstract: Our results indicate that cross-infection with P. aeruginosa between cystic fibrosis patients has occurred.

Cited by 42 publications

References 32 publications

Factors associated with infection by Pseudomonas aeruginosa in adult cystic fibrosis

Factors associated with infection by Pseudomonas aeruginosa in adult cystic fibrosis

Investigation of the algT operon sequence in mucoid and non-mucoid Pseudomonas aeruginosa isolates from 115 Scandinavian patients with cystic fibrosis and in 88 in vitro non-mucoid revertants

Challenging and emerging pathogens in cystic fibrosis

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