Typing of nosocomial strains of Serratia marcescens: comparison of pulsed-field gel electrophoresis of macrorestriction fragments with biotyping, esterase typing and ribotyping

Chetoui, Hafid; Delhalle, E; Melin, Pierrette; Struelens, Marc; Ryck, R. De; Osterrieth, Paul M.; Mol, Patrick De

doi:10.1016/s0923-2508(98)80028-4

Cited by 15 publications

(16 citation statements)

References 29 publications

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“…marcescens is recognized as a heterogeneous species that probably contains an unlimited number of different clones. 31 The stress for surviving in a hostile environment may alter the genetic structure of the bacterial population. 40 Evolutionary changes in bacterial genotypes from a common strain, the intrusion of new clones from external sources, and repeated contamination from a common source could explain the presence of different genotypes in nosocomial outbreaks.…”

Section: And Bothmentioning

confidence: 99%

“…[27][28][29][30] The study of epidemiological markers of isolated strains is an important tool in the search for the source of contamination and to attempt to prevent patient-to-patient dissemination of infection. 31 Nowadays, new molecular typing methods facilitate the recognition of epidemic strains and enhance understanding of the epidemiology of cases. [32][33][34][35][36][37][38] Nosocomial co-infections and concurrent outbreaks due to both S. marcescens and K. pneumoniae have not been reported previously.…”

Section: Introductionmentioning

confidence: 99%

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A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

Casolari

Pecorari

Fabio

et al. 2005

Journal of Hospital Infection

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Section: And Bothmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

Casolari

Pecorari

Fabio

et al. 2005

Journal of Hospital Infection

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“…However, typing methods exist for the related species Ser. marcescens in form of restriction fragment length polymorphism by pulsed-®eld gel electrophoresis (PFGE) (Miranda et al 1996;Traub et al 1996;Chetoui et al 1998), random ampli®ed polymorphic DNA (RAPD) analysis by arbitrarily primed PCR (Hejazi et al 1997) and ribotyping (Chetoui et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Diversity of antifungal and plant-associated Serratia plymuthica strains

Berg

2000

J Appl Microbiol

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A total of 21 plant-associated Serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (DGGE) of PCR-ampli®ed 16S rDNA, PCR ®ngerprints using BOX primers (BOX-PCR) and pulsed-®eld gel electrophoresis (PFGE) after digestion with SpeI.All of the investigated strains demonstrated antifungal activity in vitro against fungal pathogens while only six strains produced the antifungal antibiotic prodigiosin. Haemolytic activity and antibiotic resistance patterns were investigated to assess the risk associated with the use of isolates in plant protection. The strains were haemolytic at human-relevant temperatures. The level of resistance to antibiotics was low. This work shows that BOX-PCR and PFGE are useful ®ngerprinting methods to characterize Ser. plymuthica strains, although the discriminatory effect between the two methods differed. Computer-assisted analysis of phenotypic and genotypic features demonstrated relationships between the origin of isolation, the production of prodigiosin and the molecular ®ngerprint.

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“…Several methods have been described for typing S. marcescens during an outbreak, including phenotyping and genotyping methods [16][17][18][19][20]. Some groups [9,21] used PFGE as a highly discriminatory and reproducible method for the epidemiological investigation of S. marcescens infections.…”

Section: Discussionmentioning

confidence: 99%

Nosocomial Neonatal Outbreak of Serratia marcescens - Analysis of Pathogens by Pulsed Field Gel Electrophoresis and Polymerase Chain Reaction

Steppberger¹,

Walter²,

Claros³

et al. 2002

Infection

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This survey shows that PCR and PFGE are comparable in respect to the discrimination and reproducibility for epidemiological studies of S. marcescens strains in nosocomial outbreaks. Genotypic fingerprinting of bacterial isolates is useful and important to limit nosocomial infections. Fingerprinting sources of nosocomial infections can be traced both by PFGE and PCR. All patients infected recovered completely and the nosocomial outbreak could be stopped rapidly.

show abstract

Typing of nosocomial strains of Serratia marcescens: comparison of pulsed-field gel electrophoresis of macrorestriction fragments with biotyping, esterase typing and ribotyping

Cited by 15 publications

References 29 publications

A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

Diversity of antifungal and plant-associated Serratia plymuthica strains

Nosocomial Neonatal Outbreak of Serratia marcescens - Analysis of Pathogens by Pulsed Field Gel Electrophoresis and Polymerase Chain Reaction

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