Typing of <i>bfpA</i> Genes of Enteropathogenic <i>Escherichia coli</i> Isolated in Thailand and Japan by Heteroduplex Mobility Assay

Iida, Mariko; Yamazaki, Mitsugu; Yatsuyanagi, Jun; Ratchtrachenchai, Orn-Anong; Subpasu, Sarayoot; Okamura, Noboru; Ito, Kenitiro

doi:10.1111/j.1348-0421.2006.tb03844.x

Cited by 4 publications

(5 citation statements)

References 18 publications

(5 reference statements)

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“…For detection of enteric bacteria, real-time multiplex PCR with primers and probes, described previously ( 20 ), was used for detection of ipaH , invA , aggR , eae , STh, STp , and LT genes to detect Shigella species/enteroinvasive E. coli (EIEC), Salmonella species, enteroaggregative E. coli (EAEC), atypical enteropathogenic E. coli (EPEC), STh and STp heat-stable toxin–producing enterotoxigenic E. coli (ST-ETEC), and heat-labile toxin–producing ETEC (LT-ETEC). We modified primers published by Iida et al ( 21 ) and designed new probes to detect bfpA genes for typical EPEC. For detection of cadF genes for Campylobacter jejuni , primers and probes published by Elfving et al ( 22 ) were used.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Analysis of Prevalence, Epidemiologic Characteristics, and Clinical Characteristics of Monoinfection and Coinfection in Diarrheal Diseases in Children in Tanzania

Moyo¹,

Kommedal²,

Blomberg³

et al. 2017

American Journal of Epidemiology

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The role of interactions between intestinal pathogens in diarrheal disease is uncertain. From August 2010 to July 2011, we collected stool samples from 723 children admitted with diarrhea (cases) to 3 major hospitals in Dar es Salaam, Tanzania, and from 564 nondiarrheic children (controls). We analyzed the samples for 17 pathogens and assessed interactions between coinfections in additive and multiplicative models. At least one pathogen was detected in 86.9% of the cases and 62.8%, of the controls. Prevalence of coinfections was 58.1% in cases and 40.4% in controls. Rotavirus, norovirus genogroup II, Cryptosporidium, and Shigella species/enteroinvasive Escherichia coli were significantly associated with diarrhea both as monoinfections and as coinfections. In the multiplicative interaction model, we found 2 significant positive interactions: rotavirus + Giardia (odds ratio (OR) = 23.91, 95% confidence interval (CI): 1.21, 470.14) and norovirus GII + enteroaggregative E. coli (OR = 3.06, 95% CI: 1.17, 7.98). One significant negative interaction was found between norovirus GII + typical enteropathogenic E. coli (OR = 0.09, 95% CI: 0.01, 0.95). In multivariate analysis, risk factors for death were presence of blood in stool and severe dehydration. In conclusion, coinfections are frequent, and the pathogenicity of each organism appears to be enhanced by some coinfections and weakened by others. Severity of diarrhea was not affected by coinfections.

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Section: Methodsmentioning

confidence: 99%

Comprehensive Analysis of Prevalence, Epidemiologic Characteristics, and Clinical Characteristics of Monoinfection and Coinfection in Diarrheal Diseases in Children in Tanzania

Moyo¹,

Kommedal²,

Blomberg³

et al. 2017

American Journal of Epidemiology

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“…The absence of the Shiga toxin gene (stx) was determined by the methods of Karch and Meyer (17) and Etoh et al (8). The bfpA gene was confirmed using the primer set bfpAks/bfpAkcomas2 and the methods of Iida et al (14).…”

Section: Methodsmentioning

confidence: 99%

Distribution of Non-Locus of Enterocyte Effacement Pathogenic Island-Related Genes in Escherichia coli carrying eae from Patients with Diarrhea and Healthy Individuals in Japan

Narimatsu

Ogata²,

Makino

et al. 2010

J Clin Microbiol

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The relationship to diarrhea of genes located on the pathogenicity islands (PAI) other than the locus of enterocyte effacement (LEE) was investigated. Enteropathogenic Escherichia coli (EPEC), the retention of espC on the EspC PAI, the OI-122 genes (efa1/lifA, nleB), the phylogenetic marker gene yjaA, and the bundle-forming pilus gene bfpA on the EPEC adherence factor (EAF) plasmid were studied. E. coli strains carrying the intimin gene (eae) without the Shiga toxin gene, isolated from patients with diarrhea (n ‫؍‬ 83) and healthy individuals (n ‫؍‬ 38) in Japan, were evaluated using PCR. The genotypes of eae and espC were identified by heteroduplex mobility assay (HMA). The proportions of strains isolated from individuals with and without diarrhea that carried these genes were as follows: bfpA, 13.3 and 7.9%, respectively; espC, 25.3 and 36.8%; efa1/lifA, 32.5 and 13.2%; nleB, 63.9 and 60.5%; yjaA, 42.2 and 55.3%. Statistical significance (P < 0.05) was achieved only for efa1/lifA. The proportion of strains lacking espC and carrying efa1/lifA was higher for patient-derived strains (30.1%) than for strains from healthy individuals (13.2%), but the difference was not significant. Strains carrying both espC and efa1/lifA were rare (2 strains from patients). Statistical analyses revealed significant relationships between espC and yjaA and between efa1/lifA and nleB, as well as significant inverse relationships between espC and efa1/lifA and between efa1/lifA and yjaA. espC was found in eae HMA types a1, a2, and c2, whereas efa1/lifA was found in types b1, b2, and c1. In addition, 6 polymorphisms of espC were found. The espC, yjaA, efa1/lifA, and nleB genes were mutually dependent, and their distributions were related to eae type, findings that should be considered in future epidemiological studies.

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“…We previously reported 5 HMA‐ bfpA types (34). In this study, we identified a new type, HMA‐ bfpA type 6 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The typing of eae and bfpA was performed by HMA as previously described (34, 35). HMA is a convenient way of determining the similarity of sequences from their heteroduplex mobility in polyacrylamide gel electrophoresis (36).…”

Section: Methodsmentioning

confidence: 99%

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Classification of perA sequences and their correlation with autoaggregation in typical enteropathogenic Escherichia coli isolates collected in Japan and Thailand

Iida

Okamura

Yamazaki

et al. 2010

Microbiology and Immunology

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Enteropathogenic Escherichia coli (EPEC) strains produce a bundle-forming pilus (BFP) that mediates localized adherence (LA) to intestinal epithelial cells. The major structural subunit of the BFP is bundlin, which is encoded by the bfpA gene located on a large EAF plasmid. The perA gene has been shown to activate genes within the bfp operon. We analyzed perA gene polymorphism among typical (eaeand bfpA-positive) EPEC strains isolated from healthy and diarrheal persons in Japan (n = 27) and Thailand (n = 26) during the period 1995 to 2007 and compared this with virulence and phenotypic characteristics. Eight genotypes of perA were identified by heteroduplex mobility assay (HMA). The strains isolated in Thailand showed strong autoaggregation and had an intact perA, while most of those isolated in Japan showed weak or no autoaggregation, and had a truncated perA due to frameshift mutation. The degree of autoaggregation was well correlated with adherence to HEp-2 cells, contact hemolysis and BFP expression. Our results showed that functional deficiency due to frameshift mutation and subsequent nonsense mutation in perA reduced BFP expression in typical EPEC strains isolated in Japan.

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Typing of bfpA Genes of Enteropathogenic Escherichia coli Isolated in Thailand and Japan by Heteroduplex Mobility Assay

Cited by 4 publications

References 18 publications

Comprehensive Analysis of Prevalence, Epidemiologic Characteristics, and Clinical Characteristics of Monoinfection and Coinfection in Diarrheal Diseases in Children in Tanzania

Comprehensive Analysis of Prevalence, Epidemiologic Characteristics, and Clinical Characteristics of Monoinfection and Coinfection in Diarrheal Diseases in Children in Tanzania

Distribution of Non-Locus of Enterocyte Effacement Pathogenic Island-Related Genes in Escherichia coli carrying eae from Patients with Diarrhea and Healthy Individuals in Japan

Classification of perA sequences and their correlation with autoaggregation in typical enteropathogenic Escherichia coli isolates collected in Japan and Thailand

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