Typing of HLA-DQA1 and DQB1 using DNA single-strand conformation polymorphism

Carrington, Mary; Miller, Tracy; White, Marga B.; Gerrard, Bernard; Stewart, Claudia; Dean, Michael; Mann, Dean L.

doi:10.1016/0198-8859(92)90073-v

Cited by 66 publications

(24 citation statements)

References 7 publications

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“…The DQA1 and DQB1 genes were typed as described before, and primer sequences are provided in Web Table A (see Supplementary Information). 30 In all, 14 microsatellite markers, spanning the entire HLA region, and two flanking markers (D6S291 and D6S1281) were amplified in multiplex PCR reactions (Figure 1). Primers sequences were obtained from the Genome Database (www.gdb.org), except for markers STR1, STR2, MICB, MIB, TNFa and TAP1, which were designed in our lab (Web Table A …”

Section: Subjectsmentioning

confidence: 99%

Defining the contribution of the HLA region to cis DQ2-positive coeliac disease patients

et al. 2004

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The major genetic susceptibility to coeliac disease is contributed by the human leukocyte antigen (HLA) region. The primary association is with the HLA-DQ2 molecule, encoded by the DQA1*05 and DQB1*02 alleles, which is expressed by over 90% of patients. The aim of our study was to perform an extensive scan of the entire HLA region to determine whether there is evidence for the presence of additional HLA susceptibility genes for coeliac disease in the Dutch population, acting independently of DQ2. In all, 16 microsatellite markers and the DQA1 and DQB1 genes were genotyped in simplex cis DQ2-positive coeliac disease families and cis DQ2-positive control families. Allele frequencies of markers on phase-known DQ2-positive haplotypes transmitted to patients were compared to a combined group of DQ2-positive nontransmitted and control haplotypes, thereby controlling for the DQ2 contribution. No significant differences at any of the marker loci were detected, suggesting that DQ2 is the major HLA risk factor for coeliac disease. Individuals homozygous for DQ2 or heterozygous for DQA1*05-DQB1*02/DQA1*0201-DQB1*02 were found to be at five-fold increased risk for development of coeliac disease (Po10 À8 ). This risk seems to be conferred by the presence of a second DQB1*02 allele next to one DQA1*05-DQB1*02 haplotype, independently of the second DQA1 allele.

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Section: Subjectsmentioning

confidence: 99%

Defining the contribution of the HLA region to cis DQ2-positive coeliac disease patients

et al. 2004

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“…SSCP is based on the property that single base changes alter the conformation of single-stranded DNA, which leads to slower migration on a nondenaturing polyacrylamide gel. 12 …”

Section: Candidate Genesmentioning

confidence: 99%

Chronic viral hepatitis and the human genome

Thio¹,

Thomas²,

Carrington

2000

Hepatology

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108

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“…Dissection of the mechanisms by which HIV acquires host cell immunoregulatory proteins and the role virion-associated host cell proteins play in triggering cell death will advance our understanding of HIV pathogenesis. a The HLA-DR beta genotype of the T1, T2, TBLCL-CD4, and H9 cell lines was determined by a combination of PCR sequence-specific priming (31) and single strand-strand conformation polymorphism (8) analyses. The HLA-DR genotype of the PBMC donor was 1101, 0701.…”

Section: A Hallmark Of Hiv Infection Is the Functional Impairment Of Cd4mentioning

confidence: 99%

Differential Incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and Major Histocompatibility Complex Class I and II Molecules into Human Immunodeficiency Virus Type 1 Virions and Microvesicles: Implications for Viral Pathogenesis and Immune Regulation

Esser

Graham

Coren

et al. 2001

J Virol

151

153

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Typing of HLA-DQA1 and DQB1 using DNA single-strand conformation polymorphism

Cited by 66 publications

References 7 publications

Defining the contribution of the HLA region to cis DQ2-positive coeliac disease patients

Defining the contribution of the HLA region to cis DQ2-positive coeliac disease patients

Chronic viral hepatitis and the human genome

Differential Incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and Major Histocompatibility Complex Class I and II Molecules into Human Immunodeficiency Virus Type 1 Virions and Microvesicles: Implications for Viral Pathogenesis and Immune Regulation

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