Typing multidrug-resistant Staphylococcus aureus: conflicting epidemiological data produced by genotypic and phenotypic methods clarified by phylogenetic analysis

Jørgensen, Marte Holten; Givney, Rodney; Pegler, M.; Vickery, Alison M.; Funnell, G R

doi:10.1128/jcm.34.2.398-403.1996

Cited by 40 publications

(15 citation statements)

References 14 publications

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“…Results of a PFGE typing study of Staphylococcus aureus [55] showed that the resolution of this method exceeded that of restriction fragment length polymorphism analysis with ribosomal or toxin A DNA probes and that it did not correlated with epidemiological data.…”

Section: Discriminatory Power Of Pfgementioning

confidence: 96%

Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing ofDermatophilus congolensis

Larrasa¹,

García-Sánchez

Ambrose³

et al. 2004

FEMS Microbiology Letters

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This study aimed to evaluate molecular typing methods useful for standardization of strains in experimental work on dermatophilosis. Fifty Dermatophilus congolensis isolates, collected from sheep, cattle, horse and a deer, were analyzed by randomly amplified polymorphic DNA (RAPD) method using twenty-one different primers, and the results were compared with those obtained by typing with a pulsed field gel electrophoresis (PFGE) method using the restriction digest enzyme Sse8387I. The typeability, reproducibility and discriminatory power of RAPD and Sse8387I-PFGE typing were calculated. Both typing methods were highly reproducible. Of the two techniques, Sse8387I-PFGE was the least discriminating (Dice Index (DI), 0.663) and could not distinguish between epidemiologically related isolates, whereas RAPD showed an excellent discriminatory power (DI, 0.7694-0.9722). Overall, the degree of correlation between RAPD and PFGE typing was significantly high (r, 0.8822). We conclude that the DNA profiles generated by either RAPD or PFGE can be used to differentiate epidemiologically unrelated isolates. The results of this study strongly suggest that at least two independent primers are used for RAPD typing in order to improve its discriminatory power, and that PFGE is used for confirmation of RAPD results.

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Section: Discriminatory Power Of Pfgementioning

confidence: 96%

Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing ofDermatophilus congolensis

Larrasa¹,

García-Sánchez

Ambrose³

et al. 2004

FEMS Microbiology Letters

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“…The PFGE was performed as previously described (Jorgensen et al 1996). Briefly, bacterial colonies were inoculated in Brain Heart Infusion and then centrifuged.…”

Section: Pulsed-field Gel Electrophoresismentioning

confidence: 99%

Detection of bacteria in bovine ovarian follicular fluid

Salary

Kafi

Derakhshandeh

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: The results of the present study show that ovarian follicular fluid is not sterile in bovines. The presence of Staphylococcus aureus in follicular fluid may partly explain the occurrence of infertility in some dairy cows. AbstractThe aim of this study was to examine the sterility of follicular fluid in large ovarian follicles in dairy cows. In all, 17 samples of paired follicular fluids and uterine contents collected from post-slaughtered dairy cows were cultured to detect aerobic and facultative anaerobic bacteria. Furthermore, the origin of the bacterial isolates from samples of follicular fluid and the uteri was also investigated using PFGE analysis. Follicular fluid concentrations of lipopolysaccharides were also determined. Of 17 uterine samples, 15 (88%) were detected as contaminated. In total, nine different bacterial genera and species were identified in the uterine and follicular fluid samples. Escherichia coli was the most prevalent bacterial species isolated from the uterine samples. Out of seven isolates of Staphylococcus aureus from the uterine samples, 6 (85%) were coagulase positive. Six isolates of Staphylococcus spp. were identified in 6 out of 17 follicular fluid samples (35%). Two out of six isolates were identified as Staphylococcus aureus (33%). Our results show that ovarian follicular fluid is not sterile in the bovine. The presence of Staphylococcus aureus in follicular fluid may partly explain the occurrence of infertility in some dairy cows.

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“…PFGE. The PFGE profiles were obtained by the method introduced by Jorgensen et al (1996). Electrophoresis was performed using the CHEF Mapper system (Bio-Rad Laboratories, Hercules, CA) with a switch time increased from 2 s to 35 s over 21 h at 13°C at a constant voltage of 6 V/cm (Santos et al, 1999;de Souza Duarte et al, 2013).…”

Section: Molecular Identification and Fingerprintingmentioning

confidence: 99%

Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

Rahmdel

Shekarforoush

Hosseinzadeh

et al. 2019

Journal of Dairy Science

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Bacteriocins have attracted great attention as potential alternatives to antibiotics and chemical food additives. In the present study, 243 Staphylococcus isolates from milk samples (n = 110) of goat and sheep herds located in Fars province, Iran, were screened for antimicrobial substance production. Twenty-eight isolates showed an antagonistic activity against the indicator strain Micrococcus luteus ATCC 4698. The susceptibility of all antimicrobial substances to proteolytic enzymes allowed us to consider them as bacteriocin-like inhibitory substances (BLIS). The term BLIS is applied to uncharacterized proteinaceous antimicrobials produced by gram-positive bacteria. Based on molecular identification methods, the isolates belonged to the species Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus pseudintermedius, Staphylococcus aureus, and Staphylococcus agnetis. Pulsed-field gel electrophoresis revealed a high level of genotype diversity among the Staph. chromogenes isolates. All of the isolates harbored nukA or bsaA2 genes, suggesting that their BLIS were related to nukacin or Bsa. The antimicrobial compounds from test strains were not effective against gram-negative pathogens, including Salmonella enterica serovar Typhimurium, Escherichia coli O157:H7, and Klebsiella pneumonia as well as the indicator mold Aspergillus fumigatus. All the gram-positive targets, including Bacillus cereus, Listeria monocytogenes, Enterococcus faecalis Ef37 (a tyramine-producer strain), Lactobacillus saerimneri 30a (a histamine-producer strain), and methicillin-resistant Staph. epidermidis, were inhibited by the Staph. chromogenes isolates. Staphylococcus haemolyticus 4S12 was able to inhibit the majority of gram-positive bacteria. Listeria monocytogenes strains were the only indicators sensitive to the antimicrobial agents produced by Staph. agnetis 4S97B. The other Staphylococcus strains were ineffective on all the organisms tested. Based on their inhibitory capacities, the BLIS produced by the Staph. chromogenes isolates seem to be interesting candidates for developing novel antimicrobial agents.

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Typing multidrug-resistant Staphylococcus aureus: conflicting epidemiological data produced by genotypic and phenotypic methods clarified by phylogenetic analysis

Cited by 40 publications

References 14 publications

Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing ofDermatophilus congolensis

Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing ofDermatophilus congolensis

Detection of bacteria in bovine ovarian follicular fluid

Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

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