Typing by cytotoxic lymphocytes. Genetics of HLA‐non‐A, B, C determinants—family studies

Abstract

Some CTLs recognize HLA‐structures in a different way than antibodies, i.e. they may identify other epitopes than antibodies or may define genetically distinct structures. From population studies, in vitro educated CTLs were selected giving rise to significant lysis on PHA‐lymphoblasts in the absence of the sharing of HLA‐A, B, (C) determinants between stimulator and target lymphocytes. Based on pair‐wise correlations these CTLs form 3 clusters, identifying structures associated to HLA‐markers. Subsequent testing in 14 complete families of ± 4 children allowed the observations: (1) CML‐traits assigned on the basis of single CTLs often segregated in a non‐mendelian way or independent of HLA. (2) Assignments made on the basis of CTL‐clusters segregated with HLA. (3) in 2 informative HLA‐B/D, DR recombinant families, CML‐traits segregated with HLA‐B. (4) No haplotype could be assigned more than one CML‐trait.

Histocompatibility typing by cytotoxic lymphocytes. The HLA‐A2–28, B5‐w35 serologically cross‐reactive groups reviewed by cytotoxic lymphocytes

“…Furthermore, it is possible that a different class of determinants governed by a separate locus/loci may serve as target determinants for CTLs and that these in some instances are immuno-dominant (for a review cf. Kristensen et al 1980). Finally, occurrence of non-T cellmediated lysis was not tested for and thus cannot be excluded.…”