Typing and Clustering of Yersinia pseudotuberculosis Isolates by Restriction Fragment Length Polymorphism Analysis Using Insertion Sequences

Voskresenskaya, E. A.; Savin, Cyril; Leclercq, Alexandre; Tseneva, Galina; Carniel, Élisabeth

doi:10.1128/jcm.00397-14

Cited by 8 publications

(6 citation statements)

References 35 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HPLC-MS/MS is a reliable and robust method for BUP and HBUP analysis compared with UV detection. Meanwhile, the method of RFLP was employed restriction endonucleases to digest target specific DNA sequences [ 27 , 28 ] exhibiting high discriminatory power, low complexity, and high capacity in differentiating isolated geographical areas [ 29 ]. Mutations in the target site also tested positive in PCR-RFLP [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Genetic Polymorphisms of CYP2B6 on the Pharmacokinetics of Bupropion and Hydroxybupropion in Healthy Chinese Subjects

Zhang

Yang

et al. 2018

Med Sci Monit

View full text Add to dashboard Cite

BackgroundBupropion (BUP) is an antidepressant and its pharmacological activity is mediated by its major metabolite, hydroxybupropion (HBUP). We investigated the effects of genetic polymorphisms of CYP2B6 on BUP and HBUP to provide certain evidence on the clinical rational administration of BUP.Material/MethodsPlasma BUP and HBUP concentrations were assayed using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).ResultsA total of 23 healthy volunteers (eleven participants with CYP2B6*1/*1, 7 participants with CYP2B6*1/*6, 3 participants with CYP2B6*4/*6, and 2 participants with CYP2B6*1/*4) received orally administered 150 mg of BUP according to protocol. Blood samples were obtained up to 96 hours after administration. The whole blood was subject to genotyping by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The concentration-time curve (AUC(0®96)), maximum plasma concentration (Cmax), and terminal half-life (t1/2) values of BUP in CYP2B6*1/*4 were lower than those of CYP2B6*1/*1. By contrast, the time to Cmax (tmax) value of the former was higher than that of the latter. The HBUP AUC(0®96) values in CYP2B6*4/*6 and CYP2B6*1/*4 increased to values 1.12-fold and 1.98-fold, compared with CYP2B6*1/*1 carriers. However, the HBUP AUC(0®96) value in CYP2B6*1/*1 was 1.51-fold higher than that in CYP2B6*1/*6. Similarly, the HBUP Cmax values in CYP2B6*4/*6 and CYP2B6*1/*4 increased by 1.12-fold and 1.97-fold, whereas the HBUP Cmax value in CYP2B6*1/*6 decreased to a value 1.64-fold lower than that in CYP2B6*1/*1.ConclusionsGenetic polymorphisms of CYP2B6 influence the pharmacokinetic parameters of BUP and HBUP and thus establish rational BUP administration for Chinese patients in clinical settings.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Genetic Polymorphisms of CYP2B6 on the Pharmacokinetics of Bupropion and Hydroxybupropion in Healthy Chinese Subjects

Zhang

Yang

et al. 2018

Med Sci Monit

View full text Add to dashboard Cite

show abstract

“…Y. enterocolitica характеризуется следующими генами: ail -специфичные только для патогенных штаммов и определяющие функцию адгезии-инвазии в кишечнике макроорганизмов; yst A -детерминирующий термостабильный токсин; yst B -также термостабильный токсин, но характерный только для Y. enterocolitica 1A биотипа [8,9]. Дополнительно для изучения популяционной структуры возбудителей и внутривидового типирования практическое значение приобретают методы молекулярно-генетического типирования: плазмидный спектр, О-серогенотипирование [3], фаготипирование, риботипирование [11], IS-фингерпринтинг [12], MLVA [1] Установлено, что фенотипически гомогенная популяция Y. pseudotuberculosis характеризуется генетическим полиморфизмом по плазмидным и хромосомным факторам патогенности. Доминирующий в сибирском и дальневосточном регионах генотип Y. pseudotuberculosis представлен О:1b серотипом первой геногруппы (pYV + , ympA + , HPI -), двухплазмидным (47:82 МDа) или одноплазмидным (47 МDа) вариантами и регионально отличается спектром риботипов и фингерпринтов, а именно для Сибири характерен 30 риботип и 88 фингерпринт, для Дальнего Востока -3, 29 и 31 риботипы и 87 фингерпринт.…”

Section: Abstract: Yersinia Pseudotuberculosis Yersinia Enterocolitunclassified

Biomolecular Characteristics of Yersinia Pseudotuberculosis and Yersinia Enterocolitica Isolated in Siberia and in the Far East

Климов¹,

Klimov²,

Каримова³

et al. 2016

Бюллетень Восточно-Сибирского Научного Центра Сибирского Отделения Российской Академии Медицинских Наук

View full text Add to dashboard Cite

A special feature of Y. pseudotuberculosis strains is its biochemical uniformity irrespective of the time and location of the causative agent isolation and the existence of 21 serological variants. Y. enterocolitica is a quite a heterogeneous species and is classified into 6 biochemical types associated with 29 serological variants. 221 Y. pseudotuberculosis and 447 Y. enterocolitica strains in total isolated in Siberia and in the Far East were characterized. Y. pseudotuberculosis genotype dominating in the Siberian and Far Eastern regions is presented by О:1b serotype of the first genogroup (pYV+, ympA+, HPI-) in two- (47:82 МDа) or single-plasmid (47 МDа) variants. Ribotyping and fingerprinting revealed 8 and 10 Y. pseudotuberculosis genotypes, respectively, that indicated relative heterogeneity of the circulated strains. Regional difference of ribotypes and fingerprints was noted. 401 of 447 Y. enterocolitica strains were classified as biotype A1 including 11 serotypes (O:4,32; O:4,44; O:5; O:6,30; O:6,31; O;7,8; O:12,25; O:13,7; O:19,8; O:41,43) and 46 strains belonged to biotypes 2–4 of O:3 and O:9 serotypes. Y. enterocolitica strains of biotypes 1A were isolated both from the environments, animals and patient samples as like the representatives of biotypes 2–4. The differentiating tests of fucose and sorbose made it possible to identify two species new for the Russian Federation – Y. mollaretii and Y. bercovierii. Y. enterocolitica biotypes 2–4 carried pYV plasmid and chromosomal ail, ystA virulence genes. These strains were referred to phagotypes Х3 (2/O:9) and VIII (4/O:3) and also to phagotype Xz (3/O:3), unique for Russia. Y. enterocolitica biotype 1A containing ystB thermostable toxin gene was confirmed to be an infectious etiological agent.

show abstract

“…Yersiniosis, one of the “other infectious diarrheas” in humans, is an important foodborne zoonosis with wide range of clinical symptoms caused by the enteric pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis , which is the third most common bacterial enteritis disease in European countries ( Mecsas, 2019 ). Although human Y. pseudotuberculosis infections are less frequent than those caused by Y. enterocolitica worldwide, it has a wide animal reservoir, mostly in temperate and cold countries ( Voskresenskaya et al, 2014 ). Pseudotuberculosis in animals can lead to tuberculosis-like symptoms, including localized tissue necrosis and granulomas in the liver, spleen, and lymph nodes ( Aswal et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The Assessment on Synergistic Activity of Ebselen and Silver Ion Against Yersinia pseudotuberculosis

et al. 2022

View full text Add to dashboard Cite

Yersinia pseudotuberculosis is a foodborne zoonotic bacterium that is pathogenic to guinea pigs, rabbits, and mice. It also causes pseudotuberculosis in humans. However, it still lacked the scientific basis for control. Here, we found out that Ebselen (EbSe) exhibited synergistic antibacterial activity with silver nitrate (Ag+) against Y. pseudotuberculosis YpIII strain with high efficacy in vitro using UV-visible light absorption spectrum, 5,5’-dithiobis-(2-nitrobenzoic acid), laser scanning confocal microscope, flow cytometry, transmission electron microscopy and Western blotting assays. The depletion of total glutathione (GSH) amount and inhibition of thioredoxin reductase (TrxR) activity in thiol-dependent redox system revealed the destructiveness of EbSe-Ag+-caused intracellular oxidative stress. Furthermore, a YpIII-caused mice gastroenteritis model was constructed. EbSe-Ag+ significantly reduced bacterial loads with low toxicity. It also down-regulated the expression levels of interferon (IL)-1β and tumor necrosis factor-α, up-regulated the expression level of IL-10 on-site. All the in vivo results demonstrated the antibacterial activity and immune-modulatory property of EbSe-Ag+. Collectively, these results provided academic fundament for further analysis and development of EbSe-Ag+ as the antibacterial agents for pseudotuberculosis control.

show abstract

Typing and Clustering of Yersinia pseudotuberculosis Isolates by Restriction Fragment Length Polymorphism Analysis Using Insertion Sequences

Cited by 8 publications

References 35 publications

Effects of Genetic Polymorphisms of CYP2B6 on the Pharmacokinetics of Bupropion and Hydroxybupropion in Healthy Chinese Subjects

Effects of Genetic Polymorphisms of CYP2B6 on the Pharmacokinetics of Bupropion and Hydroxybupropion in Healthy Chinese Subjects

Biomolecular Characteristics of Yersinia Pseudotuberculosis and Yersinia Enterocolitica Isolated in Siberia and in the Far East

The Assessment on Synergistic Activity of Ebselen and Silver Ion Against Yersinia pseudotuberculosis

Contact Info

Product

Resources

About