Typical and atypical antipsychotics alter acetylcholinesterase activity and ache expression in zebrafish (Danio rerio) brain

Seibt, Kelly Juliana; Oliveira, Renata da Luz; Rico, Eduardo Pacheco; Dias, Renato Dutra; Bogo, Maurı́cio Reis; Bonan, Carla Denise

doi:10.1016/j.cbpc.2009.01.008

Cited by 24 publications

(10 citation statements)

References 56 publications

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“…2C). Similar results were obtained by Seibt et al (2009), which evaluated the in vitro and in vivo effects of other antipsychotics (such as sulpiride, olanzapine, and haloperidol) on AChE activity in zebrafish brain. Other experiments showed that injections of chlorpromazine in dogs caused a significant increase in the AChE on the brains of experimental animals (Bareggi and Giacobini, 1978).…”

Section: Chlorpromazinesupporting

confidence: 82%

“…The interference of antipsychotics with AChE is not absolutely surprising, since these drugs may interact with the lipid arrangement at the neuronal membrane, being likely to change membrane biological properties (Tessier et al, 2008). This can indeed cause changes in membrane bilayer environment, stimulating the inhibitory effect observed on acetylcholinesterase activity for in vitro experiments (Seibt et al, 2009).…”

Section: Chlorpromazinementioning

confidence: 98%

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Evaluation of ecotoxicological effects of drugs on Daphnia magna using different enzymatic biomarkers

Oliveira

Antunes

Gonçalves

et al. 2015

Ecotoxicology and Environmental Safety

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Section: Chlorpromazinesupporting

confidence: 82%

Section: Chlorpromazinementioning

confidence: 98%

Evaluation of ecotoxicological effects of drugs on Daphnia magna using different enzymatic biomarkers

Oliveira

Antunes

Gonçalves

et al. 2015

Ecotoxicology and Environmental Safety

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“…AChE has many functions beyond acetylcholine hydrolysis 25, 26 . In mammals and zebrafish, AChE regulates cell-matrix interactions in bone 27 .…”

Section: Introductionmentioning

confidence: 99%

Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

Yang

Stanley

et al. 2017

Sci Rep

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Among its other biological roles, acetylcholinesterase (AChE, EC 3.1.1.7), encoded by two ace in most insects, catalyses the breakdown of acetylcholine, thereby terminating synaptic transmission. ace1 encodes the synaptic enzyme and ace2 has other essential actions in many insect species, such as Chilo suppressalis and Plutella xylostella. The silkworm, Bombyx mori, has been domesticated for more than two thousand years and its aces have no history of pesticide exposure. Here, we investigated the functional differences between two ace genes, BmAce1 and BmAce2, in the silkworm. qPCR analysis indicated that BmAce1 is highly expressed in muscle and BmAce2 is more ubiquitously expressed among tissues and enriched in the head. Both genes were separately suppressed using chemically synthesized siRNAs. The mRNA abundance of the two ace genes was significantly reduced to about 13% – 75% of the control levels after siRNA injection. The AChE activities were decreased to 32% to 85% of control levels. Silencing BmAce2 resulted in about 26% mortality, faster and higher than the 20% in the siBmAce1-treated group. Silencing BmAce1 impacted motor control and development to a greater extent than silencing BmAce2, although both treatment groups suffered motor disability, slowed development and reduced cocoons. Both genes have essential, differing biological significance.

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“…The typical function of AChE is to hydrolyze the neurotransmitter acetylcholine to terminate synaptic transmission. But increasing evidence suggests that AChE might also have atypical functions (Jiang and Zhang, ; Seibt et al, ). In mammal and zebrafish, AChE has been reported to participate in tissue growth and development such as regulating cell–matrix interactions in bone (Inkson et al, ), participating in neuroblastoma cell adhesion and neurite outgrowth (Johnson and Moore, ), an interactive major role in neocortical development by alternative splicing (Dori et al, ), regulating AChE‐mediated axonal growth (Bigbee and Sharma, ), synaptogenesis, memory formation and stress responses (Zimmerman and Soreq, ), cell proliferation and apoptosis (Jin et al, ).…”

Section: Introductionmentioning

confidence: 99%

RNA INTERFERENCE OF TWO ACETYLCHOLINESTERASE GENES IN Plutella xylostella REVEALS THEIR DIFFERENT FUNCTIONS

Sun

2012

Arch Insect Biochem Physiol

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Acetylcholinesterase (AChE, EC 3.1.1.7) is an important enzyme with a typical function of degrading the neurotransmitter acetylcholine. Although two ace genes were reported in Plutella xylostella, their function differences remain largely unknown. The chemically synthesized siRNAs (si-Pxace1 and si-Pxace2) were injected into the second instar larvae to knock down Pxace1 and Pxace2, either respectively or simultaneously. The mRNA abundance of Pxace1 and Pxace2 was significantly reduced to 7-33.5% of the control levels at 72 h after siRNA injection. The AChE activities were significantly decreased at 96 h after treatment. Silencing of Pxace1 or Pxace2 resulted in mortality of 33.9 and 22.9%, respectively. The survivors in siRNA-treated groups had apparent growth inhibition such as reduction in larvae weights and lengths, malformation and motor retardation. Knockdown of Pxace1 apparently affected more on larvae growth than that of Pxace2, suggesting that Pxace1 had more important roles than Pxace2. Both Pxace1 and Pxace2 genes might have atypical functions in regulating larvae growth and motor ability.

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Typical and atypical antipsychotics alter acetylcholinesterase activity and ache expression in zebrafish (Danio rerio) brain

Cited by 24 publications

References 56 publications

Evaluation of ecotoxicological effects of drugs on Daphnia magna using different enzymatic biomarkers

Evaluation of ecotoxicological effects of drugs on Daphnia magna using different enzymatic biomarkers

Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

RNA INTERFERENCE OF TWO ACETYLCHOLINESTERASE GENES IN Plutella xylostella REVEALS THEIR DIFFERENT FUNCTIONS

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