Typhonium blumei extract inhibits proliferation of human lung adenocarcinoma A549 cells via induction of cell cycle arrest and apoptosis

Hsu, Hsia-Fen; Huang, Kai; Lu, Kuei-Ju; Chiou, Shu-Jiau; Yen, Jui-Hung; Chang, Chi‐Chang; Houng, Jer-Yiing

doi:10.1016/j.jep.2011.03.048

Cited by 52 publications

(37 citation statements)

References 47 publications

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“…Many plant-derived bioactive constituents, including paclitaxel (from Taxus brevifolia), camptothecin (from Camptotheca acuminata), podophyllotoxin (from Podophyllum emodi) and vinblastine (from Catharanthus roseus), have been developed as potential sources of anticancer agents (Hsu et al, 2011). Recent scientific efforts have focused on the potential roles of traditional herbs extracts as alternative and complementary medications for cancer treatment (Kim et al, 2012;Lee et al, 2010;Moon, 2012).…”

Section: Discussionmentioning

confidence: 99%

Anticancer activity ofSaussurea lappaextract by apoptotic pathway in KB human oral cancer cells

Moon

Yun

Kook

et al. 2013

Pharmaceutical Biology

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Context: Saussurea lappa Dence (Compositae) is used as a traditional herbal medicine to treat abdominal pain and tenesmus in East Asia. Current studies have shown that S. lappa has anticancer activity in divergent of cancer cells. However, the effects of S. lappa on oral cancer and its mechanisms of action have yet to be elucidated. Objective: To explore its potential chemotherapeutic effects and mechanism of cell growth inhibition on human oral cancer cells. Materials and methods: The dried roots of S. lappa were used in this study. Cell viability of KB cells was evaluated by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay after treatment with 30 mg/ml of methanol extract from the dried roots of S. lappa. To understand whether its effect on cell death is related with apoptosis pathway, we performed DNA fragmentation assay, western blot, caspase activity assay and fluorescence-activated cell sorting (FACS) analysis. Results: Treatment of S. lappa extract onto KB cells reduced cell viability significantly with an IC 50 value of 30 mg/ml. The formation of a DNA ladder was observed starting at the 24 h treatment. In western blotting analysis, the S. lappa extract induced the proteolytic processing of caspase-3, -9 and poly (ADP-ribose) polymerase, a significant increase of Bax and marked reduction of Bcl-2. We also confirmed the activation of caspase-3/-7 in living KB cells by fluorescence microscopy. Conclusion: These results suggested that S. lappa extract inhibited cell proliferation through the apoptosis pathway in KB human oral cancer cells.

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Section: Discussionmentioning

confidence: 99%

Anticancer activity ofSaussurea lappaextract by apoptotic pathway in KB human oral cancer cells

Moon

Yun

Kook

et al. 2013

Pharmaceutical Biology

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“…Extract induced apoptosis in the HeLa cell by the release of cytochrome c into cytoplasm, followed by activation of caspases and proteolytic cleavage of PARP. Studies carried out by Hsu et al (2011) showed the cytotoxic potential of Typhonium blumei extract in A549 lung cancer cells. Flow cytometric analysis demonstrated that A549 cells were arrested at the G2/M phase and apoptosis was induced by the mitochondrial pathway reducing the expression of Bcl-2 and Bcl-xL and enhancing the expression of Bax, Bad and Bak resulting in activation of caspase-9 and caspase-3.…”

Section: Mechanisms Of Apoptosismentioning

confidence: 99%

Phytoconstituents as apoptosis inducing agents: strategy to combat cancer

et al. 2015

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Advancement in the field of cancer molecular biology has aided researchers to develop various new chemopreventive agents which can target cancer cells exclusively. Cancer chemopreventive agents have proficiency to inhibit, reverse and delay process of carcinogenesis during its early and later course. Chemopreventive agents can act as antioxidative, antimutagenic/antigenotoxic, anti-inflammatory agents or via aiming various molecular targets in a cell to induce cell death. Apoptosis is a kind of cell death which shows various cellular morphological alterations such as cell shrinkage, blebbing of membrane, chromatin condensation, DNA fragmentation, formation of apoptotic bodies etc. Nowadays, apoptosis is being one of the new approaches for the identification and development of novel anticancer therapies. For centuries, plants are known to play part in daily routine from providing food to management of human health. In the last two decades, diverse phytochemicals and various botanical formulations have been characterized as agents that possess potential to execute cancer cells via inducing apoptosis. Data obtained from the research carried out globally pointed out that natural products are the potential candidates which have capability to combat cancer. In the present review, we surveyed literature on natural products which throws light on the mechanism through which these phytochemicals induce apoptosis in cancer cells.

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“…But, there are very few chemotherapeutic agents which have been found to affect G2/M phase. In the present study, alloimperatorin epoxide derivative has been found to affect G2/M cell cycle phase and as such cause G2/M cell cycle arrest (Hsu et al, 2011;Xu et al, 2011;Wilkinson et al, 2007).…”

Section: Discussionmentioning

confidence: 52%

Alloimperatorin and its epoxide derivative exhibit in vitro antitumor activity in HL-60 acute myeloid leukemia cancer cells via inducing apoptosis, cell cycle disruption and inhibition of cell migration

Chen

et al. 2016

Bangladesh J Pharmacol

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The aim of the present study was to synthesize epoxide derivative of alloimperatorin and evaluating its antitumor and apoptotic effects in acute myeloid leukemia HL-60 cells. The cytotoxic effects were demonstrated by MTT assay. Fluorescence microscopy along with flow cytometry were performed to evaluate the effect of alloimperatorin epoxide on apoptosis and cell cycle. In vitro wound healing assay was performed to study compound’s effect on cancer cell migration. The results indicated that alloimperatorin epoxide (IC50 = 32.1 µM) was much more effective in inhibiting HL-60 cancer cell growth as compared to alloimperatorin (IC50 = 128 µM). Further, alloimperatorin epoxide induced apoptosis related morphological alterations in HL-60 cells including blebbing of plasma membrane, DNA fragmentation and formation of apoptotic bodies. Alloimperatorin epoxide also led to G2/M phase cell cycle arrest and suppressed HL-60 cancer cell migration indicating that this compound may be a promising candidate for the treatment of cancer metastasis.

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Typhonium blumei extract inhibits proliferation of human lung adenocarcinoma A549 cells via induction of cell cycle arrest and apoptosis

Cited by 52 publications

References 47 publications

Anticancer activity ofSaussurea lappaextract by apoptotic pathway in KB human oral cancer cells

Anticancer activity ofSaussurea lappaextract by apoptotic pathway in KB human oral cancer cells

Phytoconstituents as apoptosis inducing agents: strategy to combat cancer

Alloimperatorin and its epoxide derivative exhibit <i>in vitro</i> antitumor activity in HL-60 acute myeloid leukemia cancer cells via inducing apoptosis, cell cycle disruption and inhibition of cell migration

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