Types of advanced optical microscopy techniques for breast cancer research: a review

U, Aparna Dravid; Mazumder, Nirmal

doi:10.1007/s10103-018-2659-6

Cited by 18 publications

(11 citation statements)

References 50 publications

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“…For breast cancers, the tumor cells were shown to display marked cellular and nuclear pleomorphism. The tumor cells, characterized by irregular size and shape, enlarged nuclei, and increased nuclearcytoplasmic ratio, infiltrated into disrupted connective tissue, leading to the loss of SHG signals [181].…”

Section: Shg-circular Dichroism (Cd)mentioning

confidence: 99%

Label-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and Applications

et al. 2019

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Non-linear optical (NLO) microscopy has proven to be a powerful tool especially for tissue imaging with sub-cellular resolution, high penetration depth, endogenous contrast specificity, pinhole-less optical sectioning capability. In this review, we discuss label-free non-linear optical microscopes including the two-photon fluorescence (TPF), fluorescence lifetime imaging microscopy (FLIM), polarization-resolved second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) techniques with various samples. The non-linear signals are generated from collagen in tissue (SHG), amylopectin from starch granules (SHG), sarcomere structure of fresh muscle (SHG), elastin in skin (TPF), nicotinamide adenine dinucleotide (NADH) in cells (TPF), and lipid droplets in cells (CARS). Again, the non-linear signals are very specific to the molecular structure of the sample and its relative orientation to the polarization of the incident light. Thus, polarization-resolved non-linear optical microscopy provides high image contrast and quantitative estimate of sample orientation. An overview of the advancements on polarization-resolved SHG microscopy including Stokes vector based polarimetry, circular dichroism, and susceptibility are also presented in this review article. The working principles and corresponding implements of above-mentioned microscopy techniques are elucidated. The potential of time-resolved TPF lifetime imaging microscopy (TP-FLIM) is explored by imaging endogenous fluorescence of NAD(P)H, a key coenzyme in cellular metabolic processes. We also discuss single laser source time-resolved multimodal CARS-FLIM microscopy using time-correlated single-photon counting (TCSPC) in combination with continuum generation from photonic crystal fiber (PCF). Using examples, we demonstrate that the multimodal NLO microscopy is a powerful tool to assess the molecular specificity with high resolution.

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Section: Shg-circular Dichroism (Cd)mentioning

confidence: 99%

Label-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and Applications

et al. 2019

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“…However, conventional histological procedures require tissue fixation, sectioning, and staining [ 7 ], [ 9 ], which is both time- and labor-intensive. To accelerate the process by which a cancer diagnosis is made, various optical imaging techniques [ 10 ]–[ 27 ] have been developed to provide real-time histological assessment. Among them, tissue staining has been a widely-adopted procedure before imaging, especially in many fluorescence microscopy techniques [ 10 ]–[ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Intraoperative Label-Free Multimodal Nonlinear Optical Imaging for Point-of-Procedure Cancer Diagnostics

Yang

Park

Marjanović

et al. 2021

IEEE J. Select. Topics Quantum Electron.

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Intraoperative imaging in surgical oncology can provide information about the tumor microenvironment as well as information about the tumor margin. Visualizing microstructural features and molecular and functional dynamics may provide important diagnostic and prognostic information, especially when obtained in real-time at the point-of-procedure. A majority of current intraoperative optical techniques are based on the use of the labels, such as fluorescent dyes. However, these exogenous agents disrupt the natural microenvironment, perturb biological processes, and alter the endogenous optical signatures that cells and the microenvironment can provide. Portable nonlinear imaging systems have enabled intraoperative imaging for realtime detection and diagnosis of tissue. We review the development of a label-free multimodal nonlinear optical imaging technique that was adapted into a portable imaging system for intraoperative optical assessment of resected human breast tissue. New developments have applied this technology to assessing needlebiopsy specimens. Needle-biopsy procedures most always precede surgical resection and serve as the first sampling of suspicious masses for diagnosis. We demonstrate the diagnostic feasibility of imaging core needle-biopsy specimens during veterinary cancer surgeries. This intraoperative label-free multimodal nonlinear optical imaging technique can potentially provide a powerful tool to assist in cancer diagnosis at the point-of-procedure.

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“…Various combinations of nonlinear imaging techniques have been applied to the problem of breast cancer delineation. 17 , 35 – 43 Most commonly it involves trying to create histology-like images—“Optical-H&E” or “Pseudo-H&E”—usually through information gathered by SHG, TPF, and/or CARS. 20 – 22 These images replicate the traditional H&E image, providing physicians with information in a way that they are used to and can easily interpret.…”

Section: Introductionmentioning

confidence: 99%

Nonlinear multispectral imaging for tumor delineation

et al. 2020

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. Significance: In breast-preserving tumor surgery, the inspection of the excised tissue boundaries for tumor residue is too slow to provide feedback during the surgery. The discovery of positive margins requires a new surgery which is difficult and associated with low success. If the re-excision could be done immediately this is believed to improve the success rate considerably. Aim: Our aim is for a fast microscopic analysis that can be done directly on the excised tissue in or near the operating theatre. Approach: We demonstrate the combination of three nonlinear imaging techniques at selected wavelengths to delineate tumor boundaries. We use hyperspectral coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and two-photon excited fluorescence (TPF) on excised patient tissue. Results: We show the discriminatory power of each of the signals and demonstrate a sensitivity of 0.87 and a specificity of 0.95 using four CARS wavelengths in combination with SHG and TPF. We verify that the information is independent of sample treatment. Conclusions: Nonlinear multispectral imaging can be used to accurately determine tumor boundaries. This demonstration using microscopy in the epi-direction directly on thick tissue slices brings this technology one step closer to clinical implementation.

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Types of advanced optical microscopy techniques for breast cancer research: a review

Cited by 18 publications

References 50 publications

Label-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and Applications

Label-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and Applications

Intraoperative Label-Free Multimodal Nonlinear Optical Imaging for Point-of-Procedure Cancer Diagnostics

Nonlinear multispectral imaging for tumor delineation

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