Type-Specific Human Papillomavirus Distribution in Invasive Squamous Cervical Carcinomas in Tunisia and Vaccine Impact

Ennaifer, Emna; Salhi, Faten; Laassili, Thalja; Fehri, Emna; Alaya, Nissaf Ben; Guizani, Ikram; Boubaker, Samir

doi:10.7314/apjcp.2015.16.15.6769

Cited by 11 publications

(15 citation statements)

References 28 publications

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“…An e cient preventive and therapeutic strategy including vaccine consideration and HPV testing needs to determine the HPV genotypes distribution in different cervical lesions. Previous Tunisian studies has been conducted on cervical cancer or women with normal pap smear [4,17]. To our Knowledge, there is few epidemiologic data concerning HPV genotypes in cervical neoplasia from Tunisia.…”

Section: Discussionmentioning

confidence: 99%

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Distribution of Human Papillomavirus in Precancerous and Cancerous Cervical Neoplasia in Tunisian Women

Rhouma

Ardhaoui

Fehri

et al. 2021

Preprint

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BackgroundHigh-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC), that is a major health problem worldwide and the second most prevalent gynecological cancer among Tunisian women. Preventive tools against CC are based on screening and prophylactic vaccines. Improving the preventive strategies as well as the therapeutic algorithms needs the understanding of HPV distribution in cervical lesions in Tunisian women. MethodsA total of 200 formalin-fixed paraffine embedded biopsies were collected in our study. DNA was extracted using Qiagen Mini prep kit. DNA quality was controlled by Beta Globin PCR. Only positive samples for Beta Globin test were used. HPV detection was performed by a nested PCR using PYGMY and GP5+/6+ primers. Genotyping was performed by Reverse Line hybridization using 31 probes. ResultsThe mean age of participants was 38.97 years and 54.5% were over 40 years. Cervical neoplasia distribution according to age showed that CINII/CINIII was observed in women over 30 years old. All samples were positive for Beta Globin PCR. Global HPV prevalence in cervical lesions was 83% (166/200). HPV was present in 65% in CINI, 82% in CINII/CINIII and 85% in CC. HR-HPV was statically associated with cervical intraepithelial neoplasia (p<10-3). HR-HPV distribution according to lesion grade and cervical cancer showed that HPV16 and HPV18 were present in all lesions. For CINII/CINIII, HPV 35 (33,33%) was the most detected type, followed by HPV18 (28,6%), HPV 45 (25%) and HPV 16 (21,7%). HPV 45(62%), HPV 18 (47%) were the most detected in CC. HPV58, 59, 66 were only detected in CC and associated with HPV45,18 and HPV16. HPV39, 31, 33, 52, 56, 68 and HPV70 was associated only with CINI. ConclusionsThese findings show that HR-HPV represents 54,6 % of all infections in all cervical lesions. Five HR-HPV (35, 18, 45,16, 51) were detected in CINII/CINIII with a high incidence of HPV 35, 18, 45, and 16 that are included in the proposed vaccines. These findings give useful information for vaccine implementation consideration as well as personalized decision algorithms of intra-epithelial cervical lesions in Tunisian women.

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Section: Discussionmentioning

confidence: 99%

“…In Northern Africa, the incidence of CC is cases with 5243 deaths annually [3]. Among Tunisian women, CC ranks fourth after breast, lung and colorectal cancer [4], with 299 new cases each year and about 200 deaths per year [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Distribution of Human Papillomavirus in Precancerous and Cancerous Cervical Neoplasia in Tunisian Women

Rhouma

Ardhaoui

Fehri

et al. 2021

Preprint

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“…This high variety of HPV types in a Romanian population is quite specific, compared to other recently published data. In this regard, HPV type 16 and 18 accounted for 92.1% of invasive cervical cancer patients sampled in Tunisia (14). Single infection was found in 48.8% of the patients.…”

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confidence: 88%

“…In sub-Saharan Africa, HPV-positivity was observed in 90.4% of cases (13) The prevalence of HPV 16 is higher in Europe, South America, and China compared to other countries; by contrast, HPV 18, 45, and 52 is more frequent in South East Asia compared to the rest of the world. Multiple infections were observed in 1.7-52.2% of the cases (8,13,14). In a retrospective study (15), de Sanjose et al analyzed paraffin-embedded samples of confirmed invasive cervical carcinoma from 38 countries worldwide.…”

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confidence: 99%

The frequency of Human Papillomavirus Genotypes in Invasive Cervical Cancer of Romanian Patients

Căpîlna

Voidăzan

Budianu

et al. 2017

Revista Romana De Medicina De Laborator

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“…In Northern Africa, the incidence of CC is 7652 cases with 5243 deaths annually [ 3 ]. Among Tunisian women, CC ranks second after breast for gynecological cancer [ 4 ], with an incidence of 3.8% (342 new cases in 2020) and about 200 deaths per year [ 5 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women

Rhouma

Ardhaoui

Fehri

et al. 2021

Infect Agents Cancer

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Background High-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC) which represents the second most prevalent gynecological cancer among Tunisian women. Preventive strategies against CC are based on prophylactic vaccines that have not yet been implemented into the national vaccination program of Tunisia. Therefore, the present study aimed to investigate the HPV genotypes distribution in cervical neoplasia in Tunisian women in order to predict the impact of using current HPV vaccines on cancer prevention in Tunisia. Methods A total of 200 formalin-fixed paraffin embedded biopsies were collected in our study. DNA was extracted using Qiagen Mini prep kit. DNA quality was controlled by Beta Globin PCR. Only positive samples for Beta Globin test were used. HPV detection was performed by a nested PCR using PYGMY and GP5+/6+ primers. Genotyping was performed by Reverse Line hybridization using 31 probes. Results The mean age of participants was 38.97 years and 75% were over 30 years. Cervical neoplasia distribution according to age showed that CINII/CINIII was observed among women over 30 years old. All samples were positive for Beta Globin PCR. Overall HPV prevalence in cervical lesions was 83% (166/200). HPV was present in 65% of CINI, 82% of CINII/CINIII and 85% of CC. HR-HPV was statistically significantly associated with cervical intraepithelial neoplasia (p < 10–3). HR-HPV distribution according to lesion grade and cervical cancer showed that HPV16 and HPV18 were present in all lesions. For CINII/CINIII, HPV 35 (37.5%) was the most detected type, followed by HPV18 (33.3%) HPV 45 (28.5%) and HPV 16 (18.9%). HPV 45(57.5%), HPV 18 (53.3%) were the most detected in CC. HPV58, 59, 68 were only detected in CC and associated with HPV45, 18 and HPV16. HPV39, 31, 33, 52, 56 and HPV70 was associated only with CINI. Conclusions Our findings can give useful information for vaccine implementation by helping the health policymakers to choose the most appropriate vaccine type in Tunisia.

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Type-Specific Human Papillomavirus Distribution in Invasive Squamous Cervical Carcinomas in Tunisia and Vaccine Impact

Cited by 11 publications

References 28 publications

Distribution of Human Papillomavirus in Precancerous and Cancerous Cervical Neoplasia in Tunisian Women

Distribution of Human Papillomavirus in Precancerous and Cancerous Cervical Neoplasia in Tunisian Women

The frequency of Human Papillomavirus Genotypes in Invasive Cervical Cancer of Romanian Patients

Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women

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