Type of protein supplement in cryopreservation solutions impacts on the degree of ultrastructural damage in frozen-thawed human oocytes

Santis, Lucia De; Nottola, Stefania Annarita; Coticchio, Giovanni; Borini, Andrea; Iussig, Benedetta; Miglietta, Selenia; Macchiarelli, Guido

doi:10.1016/j.cryobiol.2020.03.010

Cited by 7 publications

(3 citation statements)

References 55 publications

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“…In particular, significantly more oocytes were retrieved among women receiving the GnRH regimen (4 vs 3, P=0.001) and two key indicators of oocyte quality (number of mature oocytes and number of normal fertilizations) were elevated in the GHRH-treated patients, all of which are prognostic factors for live birth rate ( 30 – 33 ). In addition, although advances in cryopreservation mean that frozen embryo transfer is considered to be almost as effective as fresh transfer ( 34 ), concerns remain that embryonal damage caused during the freeze-thaw process may contribute to reduced viability ( 35 , 36 ). We can speculate that this also may have contributed to the lower CLBR associated with the PPOS regimen.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Cumulative Live Birth Rates Between GnRH-A and PPOS in Low-Prognosis Patients According to POSEIDON Criteria: A Cohort Study

Zhang

Yin

et al. 2021

Front. Endocrinol.

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ObjectiveTo compare the cumulative live birth rate (CLBR) of a gonadotropin-releasing hormone (GnRH) antagonist regimen and a progestin-primed ovarian stimulation (PPOS) regimen in low-prognosis patients according to POSEIDON criteria.DesignSingle-center, retrospective, observational study.SettingHenan Provincial People’s Hospital, Zhengzhou, ChinaPatientsWomen aged ≤40 years, with a body mass index <25 kg/m2, who underwent in vitro fertilization (IVF) or intracytoplasmic sperm microinjection (ICSI) and met POSEIDON low-prognosis criteria.InterventionGnRH or PPOS regimen with IVF or ICSI.Main Outcome MeasureCLBR per oocyte retrieval cycle.ResultsPer oocyte retrieval cycle, CLBR was significantly higher with GnRH antagonist versus PPOS (35.3% vs 25.2%; P<0.001). In multivariable logistic regression analysis, CLBR per oocyte retrieval cycle was significantly lower with PPOS versus GnRH antagonist before (OR 0.62 [95% confidence intervals (CI): 0.46, 0.82; P=0.009]) and after (OR 0.66 [95% CI: 0.47, 0.93; P=0.0172]) adjustment for age, body mass index, infertility type, infertility duration, baseline follicle stimulating hormone, anti-Müllerian hormone (AMH), antral follicle count (AFC), and insemination method. CLBR was numerically higher with the GnRH antagonist regimen than with PPOS, across all of the POSEIDON groups, and was significantly higher in patients aged ≥35 years with poor ovarian reserve [AFC <5, AMH <1.2 ng/mL] (unadjusted, P=0.0108; adjusted, P=0.0243).ConclusionIn this single-center, retrospective, cohort study, patients had a higher CLBR with a GnRH antagonist versus PPOS regimen, regardless of other attributes.

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Section: Discussionmentioning

confidence: 99%

Comparison of Cumulative Live Birth Rates Between GnRH-A and PPOS in Low-Prognosis Patients According to POSEIDON Criteria: A Cohort Study

Zhang

Yin

et al. 2021

Front. Endocrinol.

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“…In human mature oocytes, most mitochondria are physically associated with SER membranes to form agglomerates composed of variable elements of ER and multiple mitochondria called M-SER aggregates and MV complexes. M-SER aggregates are composed of anastomosing tubules of SER surrounded by several mitochondria and are mainly located in the cortical areas of the ooplasm; MV complexes are, instead, usually formed by small vesicles surrounded by mitochondria, appearing as "necklace-like" structures distributed in the ooplasm [26,[28][29][30][31][32][33][34]. Larger, spherical sacs of ER decorated with mitochondria become, instead, more numerous in human oocytes subjected to prolonged culture (in vitro matured and in vitro aged oocytes) and in oocytes sampled from older patients (reproductive aging, patients age: ≥35 years) [21,35].…”

Section: Discussionmentioning

confidence: 99%

Effects of Simulated Microgravity In Vitro on Human Metaphase II Oocytes: An Electron Microscopy-Based Study

Miglietta

Cristiano

Espinola

et al. 2023

Cells

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The Gravity Force to which living beings are subjected on Earth rules the functionality of most biological processes in many tissues. It has been reported that a situation of Microgravity (such as that occurring in space) causes negative effects on living beings. Astronauts returning from space shuttle missions or from the International Space Station have been diagnosed with various health problems, such as bone demineralization, muscle atrophy, cardiovascular deconditioning, and vestibular and sensory imbalance, including impaired visual acuity, altered metabolic and nutritional status, and immune system dysregulation. Microgravity has profound effects also on reproductive functions. Female astronauts, in fact, suppress their cycles during space travels, and effects at the cellular level in the early embryo development and on female gamete maturation have also been observed. The opportunities to use space flights to study the effects of gravity variations are limited because of the high costs and lack of repeatability of the experiments. For these reasons, the use of microgravity simulators for studying, at the cellular level, the effects, such as those, obtained during/after a spatial trip, are developed to confirm that these models can be used in the study of body responses under conditions different from those found in a unitary Gravity environment (1 g). In view of this, this study aimed to investigate in vitro the effects of simulated microgravity on the ultrastructural features of human metaphase II oocytes using a Random Positioning Machine (RPM). We demonstrated for the first time, by Transmission Electron Microscopy analysis, that microgravity might compromise oocyte quality by affecting not only the localization of mitochondria and cortical granules due to a possible alteration of the cytoskeleton but also the function of mitochondria and endoplasmic reticulum since in RPM oocytes we observed a switch in the morphology of smooth endoplasmic reticulum (SER) and associated mitochondria from mitochondria-SER aggregates to mitochondria–vesicle complexes. We concluded that microgravity might negatively affect oocyte quality by interfering in vitro with the normal sequence of morphodynamic events essential for acquiring and maintaining a proper competence to fertilization in human oocytes.

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“…In this study, ultrastructural cytoplasmic alterations in AV oocytes suggest a greater sensitivity to stress response related to vitrification. In humans, microvacuolization was observed as a hallmark of cell damage after slow freezing of oocytes from young women [ 33 ]. Cytoplasmic microvacuoles are usually rare or absent in fresh, healthy MII oocytes in humans [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Advanced Maternal Age Deteriorates the Developmental Competence of Vitrified Oocytes in Mice

Lee

Park

Yoon

et al. 2021

Cells

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Advanced maternal age (AMA) is known to be related to the decrease in the quality and quantity of oocytes. Oocyte vitrification is now considered an established assisted reproductive technology for fertility preservation. However, it remains unclear whether the oocytes in older women are more sensitive to various insults during vitrification. Thus, we evaluated whether AMA affects cellular and molecular features and developmental outcomes of oocytes after vitrification in mice. The oocytes were grouped as young fresh (YF), young vitrified/warmed (YV), aged fresh (AF), and aged vitrified/warmed (AV). The survival rate of AV oocytes was significantly lower than that of YV oocytes. The rates of fertilization, cleavage, and blastocyst formation of AV oocytes were significantly lower than those of other groups. AV oocytes were represented as aberrations in mitochondria distribution, microvacuole size, and autophagosome formation, leading to delayed embryo development in mice. This delay was associated with a reduced number of total cells and trophectoderm in the blastocyst developed from AV oocytes. Collectively, AMA exaggerates the vulnerability of oocytes to cryo-damage that occurs during vitrification in mice, suggesting that the current vitrification protocols optimized for oocytes from young females should be modified for oocytes from aged women.

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Type of protein supplement in cryopreservation solutions impacts on the degree of ultrastructural damage in frozen-thawed human oocytes

Cited by 7 publications

References 55 publications

Comparison of Cumulative Live Birth Rates Between GnRH-A and PPOS in Low-Prognosis Patients According to POSEIDON Criteria: A Cohort Study

Comparison of Cumulative Live Birth Rates Between GnRH-A and PPOS in Low-Prognosis Patients According to POSEIDON Criteria: A Cohort Study

Effects of Simulated Microgravity In Vitro on Human Metaphase II Oocytes: An Electron Microscopy-Based Study

Advanced Maternal Age Deteriorates the Developmental Competence of Vitrified Oocytes in Mice

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