Type of carbohydrate in feed affects the expression of small leucine-rich proteoglycans (SLRPs), glycosaminoglycans (GAGs) and interleukins in skeletal muscle of Atlantic cod (Gadus morhua L.)

Tingbø, Monica G.; Pedersen, Mona Elisabeth; Grøndahl, Frøy; Kolset, Svein Olav; Veiseth-Kent, Eva; Enersen, Grethe; Hannesson, Kirsten O.

doi:10.1016/j.fsi.2012.06.025

Cited by 11 publications

(12 citation statements)

References 53 publications

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“…The following results present and discuss the potential of spectroscopic analysis of connective tissue in skeletal muscle of Atlantic cod. The results are compared with biochemical results from a previous study and immunostaining.…”

Section: Resultsmentioning

confidence: 97%

“…The Atlantic cod used in this study originated from a feeding trial done by Tingbø . The Atlantic cod was ( Gadus morhus L .)…”

Section: Methodsmentioning

confidence: 99%

“…Both diets contained approximately 15% carbohydrates. The amount of protein and fat was similar in the diets . Sampling was carried out after starving the fish for 48 hours, blood glucose was measured using iSTAT apparatus (Abbot Laboratories, IL), and muscle tissue samples were collected immediately after slaughter.…”

Section: Methodsmentioning

confidence: 99%

“…The current study investigates the potential of FTIR spectroscopy to measure connective tissue components, collagen and GAGs in Atlantic cod filets. The Atlantic cod used in this study originated from a feeding trial , and by mRNA it was found that fish fed a high starch (HS) diet contained relative more collagen type I. By applying high‐performance liquid chromatography the fish fed the low starch (LS) diet was found to contain about 40% more GAGs in the connective tissue than the fish fed the HS diet.…”

Section: Introductionmentioning

confidence: 99%

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The use of Fourier‐transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.)

Sanden

Köhler

Afseth

et al. 2019

Journal of Biophotonics

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In the present study, Fourier‐transform infrared spectroscopy (FTIR) is investigated as a method to measure connective tissue components that are important for the quality of Atlantic cod filets (Gadus morhua L.). The Atlantic cod used in this study originated from a feeding trial, which found that fish fed a high starch diet contained relative more collagen type I, while fish fed a low starch (LS) diet contained relative more glycosaminoglycans (GAGs) in the connective tissue. FTIR spectra of pure commercial collagen type I and GAGs were acquired to identify spectral markers and compare them with FTIR spectra and images from connective tissue. Using principal component analysis, high and LS diets were separated due to collagen type I in the spectral region 1800 to 800 cm−1. The spatial distribution of collagen type I and GAGs were further investigated by FTIR imaging in combination with immunohistochemistry. Pixel‐wise correlation images were calculated between preprocessed connective tissue images and preprocessed pure components spectra of collagen type I and GAGs, respectively. For collagen, the FTIR images reveal a collagen distribution that closely resembles the collagen distribution as imaged by immunohistochemistry. For GAGs, the concentration is very low. Still, the FTIR images detect the most GAGs rich regions.

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Section: Resultsmentioning

confidence: 97%

“…The Atlantic cod used in this study originated from a feeding trial done by Tingbø . The Atlantic cod was ( Gadus morhus L .)…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The use of Fourier‐transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.)

Sanden

Köhler

Afseth

et al. 2019

Journal of Biophotonics

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“…ELISA was performed for measuring BpIL-10 levels in the supernatant of MO/MФ as described previously [32]. Viable E .…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of mudskipper (Boleophthalmus pectinirostris) hypoxia-inducible factor-1α (HIF-1α) and analysis of its function in monocytes/macrophages

Guan

et al. 2017

PLoS ONE

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Hypoxia-inducible factor-1α (HIF-1α) plays a critical role in immune and inflammatory responses and is important in controlling a variety of processes in monocytes and macrophages. However, very little information is available about the functions of HIF-1α in fish monocytes/macrophages (MO/MФ). In this study, the cDNA sequence of the mudskipper (Boleophthalmus pectinirostris) HIF-1α gene (BpHIF-1α) was determined. Sequence comparison and phylogenetic tree analysis showed that BpHIF-1α is clustered in the fish HIF-1α tree. Constitutive expression of BpHIF-1α mRNA was detected by real-time quantitative PCR in all tested tissues, and the expression was found to be dramatically increased in the skin, liver, spleen, and kidney after Edwardsiella tarda infection. In addition, hypoxia and infection induced the expression of the BpHIF-1α transcript and protein in MO/MФ, respectively. Hypoxia caused an increase in phagocytic and bactericidal capacity of mudskipper MO/MФ in a BpHIF-1α-dependent manner. BpHIF-1α induced an anti-inflammatory status in MO/MФ upon E. tarda infection and hypoxia. Therefore, BpHIF-1α may play a predominant role in the modulation of mudskipper MO/MФ function in the innate immune system.

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Regulatory Potential of Carbohydrates—‘Life’s Little Luxury Controls’

Steinberg

2022

Aquatic Animal Nutrition

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Type of carbohydrate in feed affects the expression of small leucine-rich proteoglycans (SLRPs), glycosaminoglycans (GAGs) and interleukins in skeletal muscle of Atlantic cod (Gadus morhua L.)

Cited by 11 publications

References 53 publications

The use of Fourier‐transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.)

The use of Fourier‐transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.)

Molecular characterization of mudskipper (Boleophthalmus pectinirostris) hypoxia-inducible factor-1α (HIF-1α) and analysis of its function in monocytes/macrophages

Regulatory Potential of Carbohydrates—‘Life’s Little Luxury Controls’

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