Type IV secretion system of Brucella spp. and its effectors

Yuan, Ke; Wang, Yufei; Li, Wengfeng; Chen, Zeliang

doi:10.3389/fcimb.2015.00072

Cited by 105 publications

(93 citation statements)

References 91 publications

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“…Interestingly, the Brucella T4SSs also lack associated T4CPs, yet only some effectors carry canonical sec signal sequences, while others do not. Precisely how the latter are translocated across the inner membrane remains an intriguing question for further study (88, 89, 90). …”

Section: Structure and Function Of Type IV Machinesmentioning

confidence: 99%

The Mosaic Type IV Secretion Systems

Christie¹

2016

EcoSal Plus

128

156

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Escherichia coli and other Gram-negative and -positive bacteria employ type IV secretion systems (T4SSs) to translocate DNA and protein substrates, generally by contact-dependent mechanisms, to other cells. The T4SSs functionally encompass two major subfamilies, the conjugation systems and the effector translocators. The conjugation systems are responsible for interbacterial transfer of antibiotic resistance genes, virulence determinants, and genes encoding other traits of potential benefit to the bacterial host. The effector translocators are used by many Gram-negative pathogens for delivery of potentially hundreds of virulence proteins termed effectors to eukaryotic cells during infection. In E. coli and other species of Enterobacteriaceae, T4SSs identified to date function exclusively in conjugative DNA transfer. In these species, the plasmid-encoded systems can be classified as the P, F, and I types. The P-type systems are the simplest in terms of subunit composition and architecture, and members of this subfamily share features in common with the paradigmatic Agrobacterium tumefaciens VirB/VirD4 T4SS. This review will summarize our current knowledge of the E. coli systems and the A. tumefaciens P-type system, with emphasis on the structural diversity of the T4SSs. Ancestral P-, F-, and I-type systems were adapted throughout evolution to yield the extant effector translocators, and information about well-characterized effector translocators also is included to further illustrate the adaptive and mosaic nature of these highly versatile machines.

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Section: Structure and Function Of Type IV Machinesmentioning

confidence: 99%

The Mosaic Type IV Secretion Systems

Christie¹

2016

EcoSal Plus

128

156

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“…In addition, the type IV secretion system of Brucella is involved in the regulation of this process[10]. In the late stage of the cycle, the replicative BCV (rBCV) derived from endoplasmic reticulum is converted into autophagic BCV (aBCV)[11]. This process requires the autophagy initiation protein Ulk-1, autophagy gene Beclin 1 , and Atg14L [12]; this shows that atypical and classical autophagy induced by Brucella share common upstream regulatory pathways[13].…”

Section: Introductionmentioning

confidence: 99%

Brucella Melitensis 16M Regulates the Effect of AIR Domain on Inflammatory Factors, Autophagy, and Apoptosis in Mouse Macrophage through the ROS Signaling Pathway

Liu

et al. 2016

PLoS ONE

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Brucellosis is a highly contagious zoonosis caused by Brucella. Brucella can invade and persist inside host cells, which results in chronic infection. We constructed AIR interference and overexpression lentiviruses to acquire AIR interference, overexpression, and rescue stable expression cell lines. We also established a Brucella melitensis 16M-infected macrophage model, which was treated with either the vehicle control or NAC (ROS scavenger N-acetylcysteine (NAC) for 0, 3, 6, 12, and 24 h. Confocal laser microscopy, transmission electron microscopy, fluorescence quantitative PCR, flow cytometry, ELISA, and Western blot were used to detect inflammation, cell autophagy and apoptosis-related protein expression levels, ROS levels, and the distribution of mitochondria. It was found that after interference and overexpression of AIR, ROS release was significantly changed, and mitochondria became abnormally aggregated. B. melitensis 16M activated the NLRP3/AIM2 inflammatory complex, and induced RAW264.7 cells to secrete IL-1β and IL-18 through the ROS pathway. B. melitensis 16M also altered autophagy-related gene expression, increased autophagy activity, and induced cell apoptosis through the ROS pathway. The results showed that after B. melitensis 16M infection, ROS induced apoptosis, inflammation, and autophagy while AIR inhibited autophagosome maturation and autophagy initiation. Autophagy negatively regulated the activation of inflammasomes and prevented inflammation from occurring. In addition, mitophagy could promote cell apoptosis.

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“…In the group of articles on pathogenicity, we find the following: O'Callaghan et al in 1999, at position 15, were the first to identify a new member of type IV secretion system family encoded by virB operon in B. suis during a screen for virulence factors. They also showed that the system is essential for the intracellular growth during infection [32].…”

Section: Discussionmentioning

confidence: 99%

The Highest Cited Papers in Brucellosis: Identification Using Two Databases and Review of the Papers’ Major Findings

Bakri

Al-Qadiri

Adwan

2018

BioMed Research International

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Citation classics represent the highest impact work in a given field. We aim to identify and analyze the most frequently cited papers on brucellosis. We used the databases Scopus and Web of Science to determine the most frequently cited papers. The most cited fifty papers in each database were identified. We then ranked the papers according to the highest citation count recorded from any of the two databases. The most frequently cited paper received 964 citations and was by DelVecchio VG et al. reporting the complete genomic sequencing of Brucella melitensis. The papers were published in 30 journals led by the “Infection and Immunity” journal and the “Veterinary Microbiology” journal (each had 7 papers). Citation classics in brucellosis were all in English except one in French and were mostly of basic science type. In addition, we noticed that 12 articles that were identified among the highest fifty articles in one database were missed by the other database and vice versa. Therefore, we suggest that searching in more than one database would detect additional citation classics.

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Type IV secretion system of Brucella spp. and its effectors

Cited by 105 publications

References 91 publications

The Mosaic Type IV Secretion Systems

The Mosaic Type IV Secretion Systems

Brucella Melitensis 16M Regulates the Effect of AIR Domain on Inflammatory Factors, Autophagy, and Apoptosis in Mouse Macrophage through the ROS Signaling Pathway

The Highest Cited Papers in Brucellosis: Identification Using Two Databases and Review of the Papers’ Major Findings

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