Type IV Pili of Acidithiobacillus ferrooxidans Are Necessary for Sliding, Twitching Motility, and Adherence

Li, Yongquan; Wan, Dongshi; Huang, Shuang-Sheng; Leng, Feifan; Yan, Lei; Ni, Yuwen; Li, Hongyu

doi:10.1007/s00284-009-9494-8

Cited by 38 publications

(32 citation statements)

References 30 publications

(45 reference statements)

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“…In addition, several flagellar proteins synthesized by CARN1 and CARN4 were identified in protein extracts (Supplementary Table S2b), as well as a protein involved in the synthesis of type 4 pilus expressed by Thiomonas sp. and known to have a role in twitching motility and adhesion (Li et al, 2010). The corresponding operon, found in the genome of this bin, was also present in the genomes of CARN5 and CARN7.…”

Section: Phylogeny and Genome Reconstructionmentioning

confidence: 82%

Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

et al. 2011

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By their metabolic activities, microorganisms have a crucial role in the biogeochemical cycles of elements. The complete understanding of these processes requires, however, the deciphering of both the structure and the function, including synecologic interactions, of microbial communities. Using a metagenomic approach, we demonstrated here that an acid mine drainage highly contaminated with arsenic is dominated by seven bacterial strains whose genomes were reconstructed. Five of them represent yet uncultivated bacteria and include two strains belonging to a novel bacterial phylum present in some similar ecosystems, and which was named 'Candidatus Fodinabacter communificans.' Metaproteomic data unravelled several microbial capabilities expressed in situ, such as iron, sulfur and arsenic oxidation that are key mechanisms in biomineralization, or organic nutrient, amino acid and vitamin metabolism involved in synthrophic associations. A statistical analysis of genomic and proteomic data and reverse transcriptase-PCR experiments allowed us to build an integrated model of the metabolic interactions that may be of prime importance in the natural attenuation of such anthropized ecosystems.

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Section: Phylogeny and Genome Reconstructionmentioning

confidence: 82%

Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

et al. 2011

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“…The adhesion force was actually a sum of various biological and physicochemical interactions, which were dependent on cell surface structures and properties. Type IV pili of A. ferrooxidans play an important role during adhesion, because they contain adhesins [29]. A. ferrooxidans highly expressed pili-associated proteins when high amounts of ferrous ions were available [30].…”

Section: Forces Between Different Cell Tips and Chalcopyrite Before Amentioning

confidence: 99%

Effect of Extracellular Polymeric Substances on Surface Properties and Attachment Behavior of Acidithiobacillus ferrooxidans

Wang

Zhu

et al. 2016

Minerals

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Bacterial contact leaching of ores is more effective than non-contact leaching. Adhesion is the first step for leaching bacteria to form a biofilm on a mineral surface. Extracellular polymeric substances (EPS) are pivotal for mediating bacterial adhesion to a substratum. In order to clarify the role of EPS, we measured the adhesion forces between chalcopyrite-, sulfur-or FeSO 4 ·7H 2 O-grown cells of Acidithiobacillus ferrooxidans and chalcopyrite by an atomic force microscope (AFM) before and after EPS removal. Surface properties of these cells were assessed by measurements of the contact angle, zeta potential, Fourier transform infrared spectroscopy (FTIR) and acid-base titration. Bacterial attachment to chalcopyrite was monitored for 140 min. The results indicate that the EPS control the surface properties of the cells. In addition, the surface properties are decisive for adhesion. The adhesion forces and the amounts of attached cells decreased dramatically after removing EPS, which was not dependent on the preculture.

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“…While the site of attachment and mechanisms for specific site detection are still unknown, the process does not appear to be random, with cells attaching to areas of surface imperfection or low-degree of crystallinity [281,291,294,[300][301][302][303][304]. Additional elements mediating adherence to surfaces include pili and S-layer proteins [170,[305][306][307].…”

Section: Attachment Of Extreme Thermoacidophiles To Surfacesmentioning

confidence: 99%

The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles

et al. 2015

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Extreme thermoacidophiles (Topt > 65 °C, pHopt < 3.5) inhabit unique environments fraught with challenges, including extremely high temperatures, low pH, as well as high levels of soluble metal species. In fact, certain members of this group thrive by metabolizing heavy metals, creating a dynamic equilibrium between biooxidation to meet bioenergetic needs and mechanisms for tolerating and resisting the toxic effects of solubilized metals. Extremely thermoacidophilic archaea dominate bioleaching operations at elevated temperatures and have been considered for processing certain mineral types (e.g., chalcopyrite), some of which are recalcitrant to their mesophilic counterparts. A key issue to consider, in addition to temperature and pH, is the extent to which solid phase heavy metals are solubilized and the concomitant impact of these mobilized metals on the microorganism's growth physiology. Here, extreme thermoacidophiles are examined from the perspectives of biodiversity, heavy metal biooxidation, metal resistance mechanisms, microbe-solid interactions, and application of these archaea in biomining operations.

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Type IV Pili of Acidithiobacillus ferrooxidans Are Necessary for Sliding, Twitching Motility, and Adherence

Cited by 38 publications

References 30 publications

Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

Effect of Extracellular Polymeric Substances on Surface Properties and Attachment Behavior of Acidithiobacillus ferrooxidans

The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles

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