Type III Secretion System–Associated Toxins, Proteases, Serotypes, and Antibiotic Resistance of<i>Pseudomonas aeruginosa</i>Isolates Associated with Keratitis

Zhu, Hua; Conibear, Tim; Bandara, Rani; Aliwarga, Yulina; Stapleton, Fiona; Willcox, Mark

doi:10.1080/02713680500536746

Cited by 59 publications

(48 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the introduction of second-generation fluoroquinolones ciprofloxacin and ofloxacin in the 1990s, the reported incidence of in vitro resistance to these antibiotics among bacteria isolated from bacterial keratitis and endophthalmitis has been steadily increased in the USA (Hwang, 2004) and India (Smitha et al, 2005). In the current study, 11% of all keratitis isolates in Australia were non-susceptible to ofloxacin, which is higher than the results of previous report (Zhu et al, 2006) in which the strains used were isolated through the years 1986 to 2004. An increasing trend of fluoroquinolone resistance was found in noncontact lens-related isolates, as the resistance rate increased from 8% (2/24) before year 2005 to 24% (4/17) from year 2006 (Choy et al, 2008).…”

Section: Discussioncontrasting

confidence: 54%

Bacteria isolated from contact and non contact lens and antibiotic susceptibility patterns of isolated Pseudomonas aeruginosa

Al-Zahrani¹

2012

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

The aim of this study was to investigate the prevalence and type of microbial contamination, associated with contact lenses and lens care accessories used by a group of contact lens wearers. Results show that a total of 178 strains were isolated, including, 100 Gram positive and 78 Gram negative bacteria. Staphylococcus epidermidis and Pseudomonas aeruginosa were the most common microorganisms isolated (25.281 and 13.483% respectively. 24 P. aeruginosa were isolated from lenses and eyes of contact lens and non-contact lens wearers. All isolates were susceptible to the tested aminoglycosides and fluoroquinolones. Aminoglycosides and fluoroquinolones (ciprofloxacin) were more efficient than β-lactams. 91.67% of the strains had intermediate-resistant to cefotaximee; 4.17% were resistant and 4.17% were sensitive to Cefotaximee. 95.83% of the strains were sensitive, while 4.17% were resistant to Imipenem. 20.83 and 12.5% of P. aeruginosa strains were resistant and sensitive to Ceftriaxone respectively, while 66.67% were moderately-resistant to ceftriaxone of the β-lactam class. All isolates were sensitive to the other tested β-lactam.

show abstract

Section: Discussioncontrasting

confidence: 54%

Bacteria isolated from contact and non contact lens and antibiotic susceptibility patterns of isolated Pseudomonas aeruginosa

Al-Zahrani¹

2012

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Other research suggested that the multidrug resistance in ocular P. aeruginosa was more commonly associated with strains having cytotoxicity and exoU gene, and belonging to serotype E (Zhu et al 2006). Recently, Zaborina et al (2006) screened consecutively isolated MDR P. aeruginosa clinical strains for their ability to disrupt the integrity of human cultured intestinal epithelial cells (Caco-2) and correlated these findings to related virulence phenotypes such as adhesiveness, motility, biofilm formation and cytotoxicity.…”

Section: Resultsmentioning

confidence: 97%

Distribution of the type III effector proteins-encoding genes among nosocomial Pseudomonas aeruginosa isolates from Bulgaria

2010

View full text Add to dashboard Cite

The aim of this study was to determine the prevalence of type III effector proteins (ExoS, ExoU, ExoT and ExoY)-encoding genes among clonally unrelated nosocomial Pseudomonas aeruginosa strains and to analyze their distribution in respect to the infection site and antimicrobial resistance. Polymerase chain reaction-based detection of the genes was performed on 176 non-duplicate P. aeruginosa isolates from three University hospitals in Sofia, previously genotyped by random amplified polymorphic DNA technique. The prevalence of the studied genes was as follows: exoS-61.9%, exoU-32.4%, exoT-100%, and exoY-85.8%. The part of P. aeruginosa strains harboring either the exoS (54.0%) or the exoU (23.8%) gene was higher (P<0.001) than that of isolates containing both genes (8.5%). The gene dissemination varied according to the infection localization. The exoU gene manifested a higher spread (P<0.001) among multidrug-resistant (MDR) than in non-MDR strains (42.6 vs 18.7%). In conclusion, the P. aeruginosa type III secretion system is present in nearly all studied isolates but the individual isolates from distinct infection sites differ in their effector genotypes. The ubiquity of type III effector proteins-encoding genes among clinical isolates is consistent with an important role for this system in P. aeruginosa pathogenesis.

show abstract

“…Previous studies have linked multidrug resistance in P. aeruginosa to TTSS phenotypes in ocular, blood, urine, and wound isolates (26, 27). Wong-Beringer and colleagues (27) studied this association by concentrating on fluoroquinolone-resistant clinical isolates of P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

Clinical outcomes of type III Pseudomonas aeruginosa bacteremia*

El-Solh

Hattemer

Hauser

et al. 2012

Critical Care Medicine

104

View full text Add to dashboard Cite

Background Pseudomonas aeruginosa bacteremia is a serious and life-threatening infection associated with high mortality. Among the multitude of virulence determinants possessed by P. aeruginosa, the type III secretion system (TTSS) has been implicated with more acute and invasive infection in respiratory diseases. However, the relationship between TTSS and clinical outcomes in P. aeruginosa bacteremia has not been investigated. Objectives To determine the association between the TTSS virulence factor in P. aeruginosa blood stream infection and 30-day mortality. Design Retrospective analysis of 85 cases of P. aeruginosa bacteremia. Setting Tertiary care hospital. Interventions Bacterial isolates were assayed in vitro for secretion of type III exotoxins (ExoU, ExoT, and ExoS). Strain-relatedness was analyzed using randomly amplified polymorphic DNA PCR genotyping. Antimicrobial susceptibilities were determined by means of the Kirby-Bauer disk-diffusion test. Measurements and Main results At least one of the TTSS proteins was detected in 37 out of the 85 isolates (44%). Septic shock was identified in 43% of bacteremic patients with TTSS+ isolates compared to 23% of patients with TTSS− isolates (p=0.12). A high frequency of resistance in the TTSS+ isolates was observed to ciprofloxacin (59%), cefipime (35%), and gentamycin (38%). There was a significant difference in the 30-day cumulative probability of death after bacteremia between secretors and nonsecretors (p=0.02). None of the TTSS+ patients who survived the first 30 days had a P. aeruginosa isolate which exhibited ExoU phenotype. Conclusions The expression of TTSS exotoxins in bacteremic isolates of P. aeruginosa confers poor clinical outcomes independent of antibiotic susceptibility profile.

show abstract

Type III Secretion System–Associated Toxins, Proteases, Serotypes, and Antibiotic Resistance ofPseudomonas aeruginosaIsolates Associated with Keratitis

Cited by 59 publications

References 48 publications

Bacteria isolated from contact and non contact lens and antibiotic susceptibility patterns of isolated Pseudomonas aeruginosa

Bacteria isolated from contact and non contact lens and antibiotic susceptibility patterns of isolated Pseudomonas aeruginosa

Distribution of the type III effector proteins-encoding genes among nosocomial Pseudomonas aeruginosa isolates from Bulgaria

Clinical outcomes of type III Pseudomonas aeruginosa bacteremia*

Contact Info

Product

Resources

About