Type II methanotrophs: A promising microbial cell-factory platform for bioconversion of methane to chemicals

Nguyen, Diep Thi Ngoc; Lee, Ok Kyung; Nguyen, Thu Thi; Lee, Eun Yeol

doi:10.1016/j.biotechadv.2021.107700

Cited by 37 publications

(18 citation statements)

References 124 publications

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“…This could be due to the different carbon assimilation pathways between the RuMP and serine pathways in Type I and Type II methanotrophs. The RuMP pathway efficiently links to the glycolytic pathway, where pyruvate is converted to organic acids, whereas the serine cycle of Type II methanotrophs has a high flux through acetyl-CoA to yield an intracellular storage compound such as polyhydroxybutyrate (PHB) under nutrient-deficient conditions ( Kalyuzhnaya et al, 2015 ; Nguyen et al, 2021 ). PHB is also a native product of M. rosea SV97 ( Wartiainen et al, 2006b ).…”

Section: Resultsmentioning

confidence: 99%

Batch Experiments Demonstrating a Two-Stage Bacterial Process Coupling Methanotrophic and Heterotrophic Bacteria for 1-Alkene Production From Methane

et al. 2022

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Methane (CH4) is a sustainable carbon feedstock for value-added chemical production in aerobic CH4-oxidizing bacteria (methanotrophs). Under substrate-limited (e.g., oxygen and nitrogen) conditions, CH4 oxidation results in the production of various short-chain organic acids and platform chemicals. These CH4-derived products could be broadened by utilizing them as feedstocks for heterotrophic bacteria. As a proof of concept, a two-stage system for CH4 abatement and 1-alkene production was developed in this study. Type I and Type II methanotrophs, Methylobacter tundripaludum SV96 and Methylocystis rosea SV97, respectively, were investigated in batch tests under different CH4 and air supplementation schemes. CH4 oxidation under either microaerobic or aerobic conditions induced the production of formate, acetate, succinate, and malate in M. tundripaludum SV96, accounting for 4.8–7.0% of consumed carbon from CH4 (C-CH4), while M. rosea SV97 produced the same compounds except for malate, and with lower efficiency than M. tundripaludum SV96, accounting for 0.7–1.8% of consumed C-CH4. For the first time, this study demonstrated the use of organic acid-rich spent media of methanotrophs cultivating engineered Acinetobacter baylyi ADP1 ‘tesA-undA cells for 1-alkene production. The highest yield of 1-undecene was obtained from the spent medium of M. tundripaludum SV96 at 68.9 ± 11.6 μmol mol Csubstrate–1. However, further large-scale studies on fermenters and their optimization are required to increase the production yields of organic acids in methanotrophs.

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Section: Resultsmentioning

confidence: 99%

Batch Experiments Demonstrating a Two-Stage Bacterial Process Coupling Methanotrophic and Heterotrophic Bacteria for 1-Alkene Production From Methane

et al. 2022

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“…In an utterly different strategy, 3-HP production has been demonstrated in the Type II methanotroph Methylosinus trichosporium OB3b. Type II methanotrophs maintain high acetyl-CoA flux, which is a particularly useful trait in deriving valuable chemicals from C1 feedstocks ( Nguyen et al, 2021 ). By engineering the malonyl-CoA pathway, methane fed cultures in nitrate mineral salt medium achieved 60.59 mg/L 3-HP ( Nguyen et al, 2020 ).…”

Section: Biochemical Precursorsmentioning

confidence: 99%

Translating advances in microbial bioproduction to sustainable biotechnology

Carruthers

Lee

2022

Front. Bioeng. Biotechnol.

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Advances in synthetic biology have radically changed our ability to rewire microorganisms and significantly improved the scalable production of a vast array of drop-in biopolymers and biofuels. The success of a drop-in bioproduct is contingent on market competition with petrochemical analogues and weighted upon relative economic and environmental metrics. While the quantification of comparative trade-offs is critical for accurate process-level decision making, the translation of industrial ecology to synthetic biology is often ambiguous and assessment accuracy has proven challenging. In this review, we explore strategies for evaluating industrial biotechnology through life cycle and techno-economic assessment, then contextualize how recent developments in synthetic biology have improved process viability by expanding feedstock availability and the productivity of microbes. By juxtaposing biological and industrial constraints, we highlight major obstacles between the disparate disciplines that hinder accurate process evaluation. The convergence of these disciplines is crucial in shifting towards carbon neutrality and a circular bioeconomy.

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“…Methanotrophs are therefore excellent candidates for CH 4 sequestration (Sahoo et al ., 2021). These capabilities enable them as cell factories for a wide range of high‐value products (Nguyen et al ., 2021). In this sense, methanotrophs have been used to synthesize polyhydroxyalkanoates for plastic sector, single cell proteins for feeding animals and lipids for biofuel production (Wang et al ., 2020).…”

Section: Fermentations Of C1 Gasesmentioning

confidence: 99%

Integrating greenhouse gas capture and C1 biotechnology: a key challenge for circular economy

Garcı́a

2021

Microbial Biotechnology

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Type II methanotrophs: A promising microbial cell-factory platform for bioconversion of methane to chemicals

Cited by 37 publications

References 124 publications

Batch Experiments Demonstrating a Two-Stage Bacterial Process Coupling Methanotrophic and Heterotrophic Bacteria for 1-Alkene Production From Methane

Batch Experiments Demonstrating a Two-Stage Bacterial Process Coupling Methanotrophic and Heterotrophic Bacteria for 1-Alkene Production From Methane

Translating advances in microbial bioproduction to sustainable biotechnology

Integrating greenhouse gas capture and C1 biotechnology: a key challenge for circular economy

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