Type I host resistance and Trichothecene Accumulation in &lt;i&gt;Fusarium&lt;/i&gt;-infected Wheat Heads

Gautam, Pravin; Dill‐Macky, Ruth

doi:10.3844/ajabssp.2011.231.241

Cited by 8 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fg produces three major toxins including DON, 3Ac-DON, and 15Ac-DON . The contents of these mycotoxins were positively associated with FHB severity for wheat . In this experiment, mycotoxins were measured for mycelium grown on PDA after 5 days.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion

Liu¹,

Chen

Zhang³

et al. 2019

J. Proteome Res.

View full text Add to dashboard Cite

Fusarium head blight (FHB) mainly resulting from Fusarium graminearum (Fg) Schwabe is a notorious wheat disease causing huge losses in wheat production globally. Fg also produces mycotoxins, which are harmful to human and domestic animals. In our previous study, we obtained two Fg mutants, TPS1 − and TPS2 − , respectively, with a single deletion of trehalose 6-phosphate synthase (TPS1) and trehalose 6-phosphate phosphatase (TPS2) compared with the wild type (WT). Both mutants were unable to synthesize trehalose and produced fewer mycotoxins. To understand the other biochemical changes induced by TPS gene deletion in Fg, we comprehensively analyzed the metabolomic differences between TPS − mutants and the WT using NMR together with gas chromatography−flame ionization detection/mass spectrometry. The expression of some relevant genes was also quantified. The results showed that TPS1 − and TPS2 − mutants shared some common metabolic feature such as decreased levels for trehalose, Val, Thr, Lys, Asp, His, Trp, malonate, citrate, uridine, guanosine, inosine, AMP, C10:0, and C16:1 compared with the WT. Both mutants also shared some common expressional patterns for most of the relevant genes. This suggests that apart from the reduced trehalose biosynthesis, both TPS1 and TPS2 have roles in inhibiting glycolysis and the tricarboxylic acid cycle but promoting the phosphopentose pathway and nucleotide synthesis; the depletion of either TPS gene reduces the acetyl-CoAmediated mycotoxin biosynthesis. TPS2 − mutants produced more fatty acids than TPS1 − mutants, suggesting different roles for TPS1 and TPS2, with TPS2 − mutants having impaired trehalose biosynthesis and trehalose 6-phosphate accumulation. This may offer opportunities for developing new fungicides targeting trehalose biosynthesis in Fg for FHB control and mycotoxin reduction in the FHB-affected cereals.

show abstract

Section: Resultsmentioning

confidence: 99%

Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion

Liu¹,

Chen

Zhang³

et al. 2019

J. Proteome Res.

View full text Add to dashboard Cite

show abstract

“…It is well known that F. graminearum (FG) produces type-B trichothecene mycotoxins which typically include deoxynivalenol (DON) and its two derivatives 15-acetyl-deoxynivalenol (15Ac-DON) and 3-acetyl-deoxynivalenol (3Ac-DON); 15Ac-DON is much more toxic to plants than DON (Desjardins et al, 1993 ; Kimura et al, 2007 ). The levels of these mycotoxins positively associate with severity of FG-caused FHB in wheat (Gautam and Dill-Macky, 2011 ). Therefore, the levels of these mycotoxins in plant tissues generally have inverse correlation with FG resistance of the given plants.…”

Section: Resultsmentioning

confidence: 99%

Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana

et al. 2018

View full text Add to dashboard Cite

Fusarium head blight disease resulting from Fusarium graminearum (FG) infection causes huge losses in global production of cereals and development of FG-resistant plants is urgently needed. To understand biochemistry mechanisms for FG resistance, here, we have systematically investigated the plant metabolomic phenotypes associated with FG resistance for transgenic Arabidopsis thaliana expressing a class-I chitinase (Chi), a Fusarium-specific recombinant antibody gene (CWP2) and fused Chi-CWP2. Plant disease indices, mycotoxin levels, metabonomic characteristics, and expression levels of several key genes were measured together with their correlations. We found that A. thaliana expressing Chi-CWP2 showed higher FG resistance with much lower disease indices and mycotoxin levels than the wild-type and the plants expressing Chi or CWP2 alone. The combined metabonomic and quantitative RT-PCR analyses revealed that such FG-resistance was closely associated with the promoted biosynthesis of secondary metabolites (phenylpropanoids, alkanoids) and organic osmolytes (proline, betaine, glucose, myo-inositol) together with enhanced TCA cycle and GABA shunt. These suggest that the concurrently enhanced biosyntheses of the shikimate-mediated secondary metabolites and organic osmolytes be an important strategy for A. thaliana to develop and improve FG resistance. These findings provide essential biochemical information related to FG resistance which is important for developing FG-resistant cereals.

show abstract

“…However, Butte86Ada‐11 was collected in Norman County in 1995 and 81‐2 from Manhomen County in 2002. Information on pathogenicity and mycotoxin production of these isolates can be found in Gautam and Dill‐Macky (2011, 2012a,b). Isolates were cultured in Petri plates on mung bean agar (MBA) and the macroconidia harvested from 7‐day‐old cultures as described by Gautam and Dill‐Macky (2012a).…”

Section: Methodsmentioning

confidence: 99%

Free Water Can Leach Mycotoxins from Fusarium‐infected Wheat Heads

Gautam

Dill‐Macky²

2012

Journal of Phytopathology

View full text Add to dashboard Cite

The objective of this study was to examine the impact of free environmental moisture, such as from rainfall, on disease development and mycotoxin production and accumulation in planta. In greenhouse experiments in 2009, two single Fusarium graminearum isolates were used to inoculate spikes of three wheat cultivars: ‘Alsen’, ‘2375’ and ‘Wheaton’ at anthesis. On each wetting event/sampling day (7, 14, 21 or 28 days after inoculation), FHB severity was assessed and five pots of each of the two cultivar/isolate treatments were subjected to a wetting event. At the end of the wetting event, the spikes were sampled both from the plants that received the wetting treatment and those that did not and analysed for mycotoxins. Run‐off water samples were also taken 3 h after the start of irrigation and immediately after the wetting treatment concluded and analysed for mycotoxins. The results showed despite statistically similar FHB severity, the levels of DON and other mycotoxins detected were significantly lower in the plants receiving a single wetting event compared to the control. The levels of DON in wetted plants were lower up to 36% in ‘Alsen’, 52% in ‘2375’ and 41% in ‘Wheaton’ compared to that of corresponding controls. DON and 15‐ADON were also detected in run‐off water from the inoculated heads of all cultivars examined. Based on the results of this study, it can be concluded that DON and its derivatives produced in planta can be leached out from the host tissues by free water on contact with plant surfaces.

show abstract

Type I host resistance and Trichothecene Accumulation in <i>Fusarium</i>-infected Wheat Heads

Cited by 8 publications

References 31 publications

Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion

Metabolic Changes of Fusarium graminearum Induced by TPS Gene Deletion

Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana

Free Water Can Leach Mycotoxins from Fusarium‐infected Wheat Heads

Contact Info

Product

Resources

About