Type I-F CRISPR-PAIR platform for multi-mode regulation to boost extracellular electron transfer in Shewanella oneidensis

Chen, Yaru; Cheng, Meijie; Song, Hao; Cao, Yingxiu

doi:10.1016/j.isci.2022.104491

Cited by 6 publications

(8 citation statements)

References 54 publications

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“…This system can up-regulate the gene expression when targeting specific sites upstream of the gene and suppress gene expression when targeting open reading frames. 126 Both studies demonstrated two genomic editing tools for constructing recombinant EABs, providing a reference for the development of more sensitive and precise genetic tools for multigene targeting and regulation.…”

Section: Transposon Mutagenesismentioning

confidence: 99%

Engineering extracellular electron transfer pathways of electroactive microorganisms by synthetic biology for energy and chemicals production

Zhang,

Li,

Liu

et al. 2024

Chem. Soc. Rev.

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Section: Transposon Mutagenesismentioning

confidence: 99%

Engineering extracellular electron transfer pathways of electroactive microorganisms by synthetic biology for energy and chemicals production

Zhang,

Li,

Liu

et al. 2024

Chem. Soc. Rev.

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“…In addition, the type I-F system from P. aeruginosa was also repurposed to achieve gene activation by fusing the activator RpoD to the Cas7 protein which functions as several copies to bind the protospacer in the Cascade. As tested in the model electroactive microorganism Shewanella oneidensis , crRNA-guided Cascade targeting the prioritized loci upstream of the transcription start site successfully activated the downstream gene expression [103] . This system was also demonstrated as a dual-regulation tool since it can be used to repress gene expression by binding with ORF regions of target genes as well [103] .…”

Section: Type I Crispr-cas-mediated Gene Regulationmentioning

confidence: 99%

“…As tested in the model electroactive microorganism Shewanella oneidensis , crRNA-guided Cascade targeting the prioritized loci upstream of the transcription start site successfully activated the downstream gene expression [103] . This system was also demonstrated as a dual-regulation tool since it can be used to repress gene expression by binding with ORF regions of target genes as well [103] .…”

Section: Type I Crispr-cas-mediated Gene Regulationmentioning

confidence: 99%

Type I CRISPR-Cas-mediated microbial gene editing and regulation

Xu,

Chen,

et al. 2023

AIMSMICRO

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<abstract> <p>There are six major types of CRISPR-Cas systems that provide adaptive immunity in bacteria and archaea against invasive genetic elements. The discovery of CRISPR-Cas systems has revolutionized the field of genetics in many organisms. In the past few years, exploitations of the most abundant class 1 type I CRISPR-Cas systems have revealed their great potential and distinct advantages to achieve gene editing and regulation in diverse microorganisms in spite of their complicated structures. The widespread and diversified type I CRISPR-Cas systems are becoming increasingly attractive for the development of new biotechnological tools, especially in genetically recalcitrant microbial strains. In this review article, we comprehensively summarize recent advancements in microbial gene editing and regulation by utilizing type I CRISPR-Cas systems. Importantly, to expand the microbial host range of type I CRISPR-Cas-based applications, these structurally complicated systems have been improved as transferable gene-editing tools with efficient delivery methods for stable expression of CRISPR-Cas elements, as well as convenient gene-regulation tools with the prevention of DNA cleavage by obviating deletion or mutation of the Cas3 nuclease. We envision that type I CRISPR-Cas systems will largely expand the biotechnological toolbox for microbes with medical, environmental and industrial importance.</p> </abstract>

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“…For instance, the CRISPR method can be utilized to accomplish coordinated transcription and translation regulation of target genes in MR-1. 20 genes in MR-1 enables the reduction of cross-linking between the peptidoglycan layer and the outer membrane, thus facilitating the formation of outer membrane vesicles and significantly improving the power output density. 21 The base editing toolbox can be employed for genomewide functional validation via gene inactivation.…”

Section: ■ Introductionmentioning

confidence: 99%

Efficient Enhancement of Extracellular Electron Transfer in Shewanella oneidensis MR-1 via CRISPR-Mediated Transposase Technology

Lin,

Cheng,

et al. 2024

ACS Synth. Biol.

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Electroactive bacteria, exemplified by Shewanella oneidensis MR-1, have garnered significant attention due to their unique extracellular electron-transfer (EET) capabilities, which are crucial for energy recovery and pollutant conversion. However, the practical application of MR-1 is constrained by its EET efficiency, a key limiting factor, due to the complexity of research methodologies and the challenges associated with the practical use of gene editing tools. To address this challenge, a novel gene integration system, INTEGRATE, was developed, utilizing CRISPR-mediated transposase technologies for precise genomic insertion within the S. oneidensis MR-1 genome. This system facilitated the insertion of extensive gene segments at different sites of the Shewanella genome with an efficiency approaching 100%. The inserted cargo genes could be kept stable on the genome after continuous cultivation. The enhancement of the organism’s EET efficiency was realized through two primary strategies: the integration of the phenazine-1-carboxylic acid synthesis gene cluster to augment EET efficiency and the targeted disruption of the SO3350 gene to promote anodic biofilm development. Collectively, our findings highlight the potential of utilizing the INTEGRATE system for strategic genomic alterations, presenting a synergistic approach to augment the functionality of electroactive bacteria within bioelectrochemical systems.

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Type I-F CRISPR-PAIR platform for multi-mode regulation to boost extracellular electron transfer in Shewanella oneidensis

Cited by 6 publications

References 54 publications

Engineering extracellular electron transfer pathways of electroactive microorganisms by synthetic biology for energy and chemicals production

Engineering extracellular electron transfer pathways of electroactive microorganisms by synthetic biology for energy and chemicals production

Type I CRISPR-Cas-mediated microbial gene editing and regulation

Efficient Enhancement of Extracellular Electron Transfer in Shewanella oneidensis MR-1 via CRISPR-Mediated Transposase Technology

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