Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts <i>In Vitro</i>

Lombardi, Francesca; Palumbo, Paola; Augello, Francesca Rosaria; Giusti, Ilaria; Dolo, Vincenza; Guerrini, Luca; Cifone, Maria Grazia; Giuliani, Maurizio; Cinque, Benedetta

doi:10.1155/2020/6093974

Cited by 5 publications

(3 citation statements)

References 73 publications

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“…As already reported [28,29], the fibroblasts basally express the α-SMA protein, which contributes to cell-generated mechanical tension, and whose expression increases in activated fibroblasts, improving their contractile activity. To verify whether the increase in collagen synthesis was associated with fibroblast contraction activity, the expression of α-SMA in the adult and aged HDFs treated with increasing concentrations of K was investigated using the Western blot method.…”

Section: Effect Of K Formulation On Fibroblast Contraction and Migrat...supporting

confidence: 63%

Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts

Augello,

Lombardi,

Artone

et al. 2023

Biomedicines

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Skin aging is a dynamic process that determines structural alterations in ECM and reduction in dermal fibroblasts. The recent availability on the market of an innovative polycomponent formulation (KARISMA Rh Collagen® FACE, K) containing noncrosslinked high-molecular-weight hyaluronic acid (HMW-HA), a human recombinant polypeptide of collagen-1 alpha chain, and carboxymethyl cellulose (CMC), attracted our scientific interest in evaluating its biomolecular effects on human dermal adult and aged fibroblasts. After treatment with increasing K concentrations, cell proliferation, collagen I, prolyl 4-hydroxylase (P4HA1), an essential protein in collagen biosynthesis, and α-SMA levels were assessed. The fibroblast contractility, TGF-β1 levels, and oxidative stress markers were also evaluated. K formulation exposure led to a significant and dose-dependent increase in the proliferation and migration of adult fibroblasts. Of note, the K exposure counteracted the H2O2-induced aging by promoting cell proliferation, reducing β-galactosidase activity, and neutralizing the aging-associated oxidative damage. Moreover, an increase in collagen I, P4HA1, α-SMA, TGF-β1 levels, and improved contractility of adult and aged fibroblasts were observed after treatment. Overall, our results show evidence that the K treatment is efficacious in improving biological functions in adult fibroblasts and suppressing the biomolecular events associated with H2O2-induced cellular aging, thus supporting the regenerative and bio-revitalizing action of the K formulation helpful in preventing or treating skin aging.

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Section: Effect Of K Formulation On Fibroblast Contraction and Migrat...supporting

confidence: 63%

Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts

Augello,

Lombardi,

Artone

et al. 2023

Biomedicines

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“…When fibroblasts are changed to activated myofibroblasts that express α-SMA, the expression of collagen type I and collagen type III increases [ 49 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

The Extracellular Matrix Vitalizer RATM Increased Skin Elasticity by Modulating Mitochondrial Function in Aged Animal Skin

Byun

Batsukh

et al. 2023

Antioxidants

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Oxidative stress-induced cellular senescence and mitochondrial dysfunction result in skin aging by increasing ECM levels-degrading proteins such as MMPs, and decreasing collagen synthesis. MMPs also destroy the basement membrane, which is involved in skin elasticity. The extracellular matrix vitalizer RATM (RA) contains various antioxidants and sodium hyaluronate, which lead to skin rejuvenation. We evaluated whether RA decreases oxidative stress and mitochondrial dysfunction, eventually increasing skin elasticity in aged animals. Oxidative stress was assessed by assaying NADPH oxidase activity, which is involved in ROS generation, and the expression of SOD, which removes ROS. NADPH oxidase activity was increased in aged skin and decreased by RA injection. SOD expression was decreased in aged skin and increased by RA injection. Damage to mitochondrial DNA and mitochondrial fusion markers was increased in aged skin and decreased by RA. The levels of mitochondrial biogenesis markers and fission markers were decreased in aged skin and increased by RA. The levels of NF-κB/AP-1 and MMP1/2/3/9 were increased in aged skin and decreased by RA. The levels of TGF-β, CTGF, and collagen I/III were decreased in aged skin and increased by RA. The expression of laminin and nidogen and basement membrane density were decreased in aged skin and increased by RA. RA increased collagen fiber accumulation and elasticity in aged skin. In conclusion, RA improves skin rejuvenation by decreasing oxidative stress and mitochondrial dysfunction in aged skin.

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“…Lombardi el al. reported that a type I collagen could induce the collagen synthesis as well as the differentiation in a fibroblast cell line 18 . So it could facilitate the rearrangement and improvement of existing collagen and elastin fibers, accompanying with hyaluronic acid, and lead to advanced elasticity properties (R2 and R5).…”

Section: Discussionmentioning

confidence: 99%

Tolerability and efficacy assessment of an oral collagen supplement for the improvement of biophysical and ultrasonographic parameters of skin in middle eastern consumers

Samadi

Movaffaghi

Kazemi

et al. 2023

J of Cosmetic Dermatology

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Background Topical skin care products often do not reach the deeper layers of the skin, and oral hydrolyzed collagen is one of the newest and most popular systemic supplementations for skin rejuvenation. However, there are limited information in case of Middle Eastern consumers Objective The purpose of this study was to evaluate the tolerability and efficacy of an oral collagen supplement for improvement of skin elasticity, hydration, and roughness in Middle Eastern consumers. Methods and Materials It was a 12‐week, before‐after clinical study, conducted on 20 participants (18 women and 2 men) aged 44.15 ± 5.36 years with skin type III–IV. Skin elasticity parameters (R0, R2, R5, and R7), skin hydration and friction, as well as the thickness and echo density of the dermis, were measured after six and 12 weeks daily intake of the study product, as well as 4 weeks after stopping its use (week 16). Participants' satisfaction was assessed on the basis of their answers to the standard questionnaire, and tolerability of the product was assessed by monitoring the adverse effects. Results A significant improvement was detected in R2, R5, and skin friction at week 12 (p‐values 0.041, 0.012 and <0.01, respectively). At week 16, the values remained at an increased level, which indicates the persistence of the results. The increase of dermis density in week 16 was also significant (p‐value = 0.03). Moderate overall satisfaction was reported with the treatment, and a few gastrointestinal complications were reported. Conclusion The study demonstrated that oral collagen peptides could significantly improve the skin elasticity, roughness, and dermis echo density, and they also proved to be safe and well‐tolerated.

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Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro

Cited by 5 publications

References 73 publications

Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts

Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts

The Extracellular Matrix Vitalizer RATM Increased Skin Elasticity by Modulating Mitochondrial Function in Aged Animal Skin

Tolerability and efficacy assessment of an oral collagen supplement for the improvement of biophysical and ultrasonographic parameters of skin in middle eastern consumers

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