Type I and type II cytokeratin cDNAs from the zebrafish (Danio rerio) and expression patterns during early development

Chua, Kim Lee; Lim, Tit Meng

doi:10.1046/j.1432-0436.2000.066001031.x

Cited by 23 publications

(40 citation statements)

References 44 publications

(66 reference statements)

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“…The hhKer1 cDNA codes for a predicted protein with an MW and pI within the expected values for vertebrate type I keratins (Moll et al 1982;Fuchs and Weber 1994;Conrad et al 1998;Schaffeld et al 1998Schaffeld et al , 2002bChua and Lim 2000;Suzuki et al 2001;Watanabe et al 2001Watanabe et al , 2002Ishida et al 2003) and possesses the characteristic tripartite structure of all vertebrate type I keratins (Fuchs and Weber 1994;Kirfel et al 2003). Computational analysis in ProDom (Bru et al 2005) has revealed that hhKer1 contains all the elements of a functional type I keratin ( Fig.…”

Section: Halibut Larval Epidermal Keratin Type I Genementioning

confidence: 95%

“…Recently, a comparison between keratin cDNA sequences isolated from zebrafish (Danio rerio) and Tetraondon nigroviridis has revealed that, in zebrafish, more type I keratin genes exist and that they seem to have resulted from tandem duplication events (Krushna Padhi et al 2006), suggesting that keratin I genes in teleost have different evolutionary histories. Several keratins have been described in teleost fish (Imboden et al 1997;Chua and Lim 2000;Martorana et al 2001;Schaffeld et al 2002aSchaffeld et al ,b, 2003 and the putative genes corresponding to all Fugu keratins have been identified in silico (Zimek et al 2003). The expression profile of some of these teleost keratin genes has been analysed during zebrafish embryonic development (Imboden et al 1997;Chua and Lim 2000) but no information exists about keratin expression during the larval period, during the larvaljuvenile transition or even in adult skin.…”

Section: Introductionmentioning

confidence: 99%

“…Several keratins have been described in teleost fish (Imboden et al 1997;Chua and Lim 2000;Martorana et al 2001;Schaffeld et al 2002aSchaffeld et al ,b, 2003 and the putative genes corresponding to all Fugu keratins have been identified in silico (Zimek et al 2003). The expression profile of some of these teleost keratin genes has been analysed during zebrafish embryonic development (Imboden et al 1997;Chua and Lim 2000) but no information exists about keratin expression during the larval period, during the larvaljuvenile transition or even in adult skin. Teleosts represent an interesting group in which to study skin, as the entire adult body skin surface is covered in scales, the epidermis is composed of live cells and no layer of keratinized dead cells is present.…”

Section: Introductionmentioning

confidence: 99%

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Molecular, cellular and histological changes in skin from a larval to an adult phenotype during bony fish metamorphosis

et al. 2006

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Developmental models for skin exist in terrestrial and amphibious vertebrates but there is a lack of information in aquatic vertebrates. We have analysed skin epidermal development of a bony fish (teleost), the most successful group of extant vertebrates. A specific epidermal type I keratin cDNA (hhKer1), which may be a bony-fish-specific adaptation associated with the divergence of skin development (scale formation) compared with other vertebrates, has been cloned and characterized. The expression of hhKer1 and collagen 1alpha1 in skin taken together with the presence or absence of keratin bundle-like structures have made it possible to distinguish between larval and adult epidermal cells during skin development. The use of a flatfish with a well-defined larval to juvenile transition as a model of skin development has revealed that epidermal larval basal cells differentiate directly to epidermal adult basal cells at the climax of metamorphosis. Moreover, hhKer1 expression is downregulated at the climax of metamorphosis and is inversely correlated with increasing thyroxin levels. We suggest that, whereas early mechanisms of skin development between aquatic and terrestrial vertebrates are conserved, later mechanisms diverge.

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Section: Halibut Larval Epidermal Keratin Type I Genementioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular, cellular and histological changes in skin from a larval to an adult phenotype during bony fish metamorphosis

et al. 2006

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“…The identification in zebrafish of homologs of human genes that are involved in epidermal tissues, such as p63 (also known as tp63 -ZFIN) (Bakkers et al, 2002;Lee and Kimelman, 2002), rbp4 (Tingaud-Sequeira et al, 2006) and keratin isoforms (Imboden et al, 1997;Chua and Lim, 2000;Martorana et al, 2001;Thisse and Thisse, 2004), suggests that genetic conservation exists between zebrafish and mammals. Furthermore, the identification of mutants such as hai1 (spint1) (Carney et al, 2007;Mathias et al, 2007), penner (lgl2) (Sonawane et al, 2005), lama5 (Webb et al, 2007) and psoriasis (Webb et al, 2008) is providing more clues as to the genetic program of epidermal development in zebrafish.…”

Section: Introductionmentioning

confidence: 99%

The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish

et al. 2009

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Epidermal hyperproliferation and inflammation are hallmarks of the human condition psoriasis. Here, we report that a zebrafish line with a mutation in the cargo adaptor protein Clint1 exhibits psoriasis-like phenotypes including epithelial hyperproliferation and leukocyte infiltration. Clint1 is an ENTH domain-containing protein that binds SNARE proteins and functions in vesicle trafficking; however, its in vivo function in animal models has not been reported to date. The clint1 mutants exhibit chronic inflammation characterized by increased Interleukin 1β expression, leukocyte infiltration, bidirectional trafficking and phagocytosis of cellular debris. The defects in clint1 mutants can be rescued by expression of zebrafish clint1 and can be phenocopied with clint1-specific morpholinos, supporting an essential role for Clint1 in epidermal development. Interaction studies suggest that Clint1 and Lethal giant larvae 2 function synergistically to regulate epidermal homeostasis. Accordingly, clint1 mutants show impaired hemidesmosome formation, loss of cell-cell contacts and increased motility suggestive of epithelial to mesenchymal transition. Taken together, our findings describe a novel function for the ENTH domain protein Clint1 in epidermal development and inflammation and suggest that its deficiency in zebrafish generates a phenotype that resembles the human condition psoriasis.

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“…Cell types of the teleost developing skin may be unambiguously identified with molecular markers or after they display their characteristic mature forms. For example, keratins may be used as marker for keratinocyte differentiation but some of them are also expressed in the EVL (Imboden et al, 1997;Chua and Lim, 2000;Gong et al, 2002;Wang et al, 2006). In situ hybridization experiments have also revealed that embryonic epidermal cells are able to start the expression type I collagen ␣2 chain (col1a2) transcript between 24 and 48 hpf to at least 26 dpf (Le Guellec et al, 2004).…”

Section: Epidermis Molecular Patterning and The Role Of Rbp4 Apoeb mentioning

confidence: 99%

Epidermal transient down‐regulation of retinol‐binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development

Tingaud‐Sequeira¹,

Forgue²,

André³

et al. 2006

Developmental Dynamics

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Very little is known about the molecular control of skin patterning and scale morphogenesis in teleost fish. We have found radially symmetrical epidermal placodes with down-regulation of retinol-binding protein 4 (rbp4) expression during the initial paired fin and scale morphogenesis in zebrafish (Danio rerio). This finding may be related to changes in keratinocyte cytodifferentiation and/or the integument retinoid metabolism. rbp4 transcripts are expressed afterward in the central epidermis of the scale papilla and gradually extend to the epidermis, covering the growing scale, whereas no transcripts were detected in posterior margin epidermis. In contrast, induction of apolipoprotein Eb (apoeb) and up-regulation of estrogen receptor 2a (esr2a) transcripts were observed in the epidermis at initiator sites of zebrafish ectodermal/dermal appendage morphogenesis. This expression was maintained in the posterior margin epidermis of the formed scales. esr2a was also strongly expressed in neuromasts, whereas no rbp4 and apoeb transcripts were detected in these mechanosensory structures. The observed epidermal molecular events suggest that epidermis patterning is due to an activator-inhibitor mechanism operational at epidermal-dermal interaction sites. rbp4 transcript expression was also strongly down-regulated by 1-phenyl-2-thio-urea (PTU). As this inhibitor is commonly used to block obscuring pigmentation during in situ hybridization studies, this finding suggests that PTU should be used with caution, particularly in studying skin development. Developmental Dynamics 235:3071-3079, 2006.

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Type I and type II cytokeratin cDNAs from the zebrafish (Danio rerio) and expression patterns during early development

Cited by 23 publications

References 44 publications

Molecular, cellular and histological changes in skin from a larval to an adult phenotype during bony fish metamorphosis

Molecular, cellular and histological changes in skin from a larval to an adult phenotype during bony fish metamorphosis

The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish

Epidermal transient down‐regulation of retinol‐binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development

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