TYPE CLASSIFICATION OF CANDIDA SPECIES OBTAINED FROM BLOOD SAMPLES BY PCR AND DNA SEQUENCE ANALYSIS

Abstract

Derniatomycosis is a very important long-term disease. Different drugs are used for cure by local and/or oral way. Especially the patients prefer oral antimycotics as griseofulvin, ketoconazole, fluconazole, itraconazole and terbinafine because of the disease's zoonotic characteristic and long-term asking cure. Because of these drugs' hepatotoxic and nephrotoxic influences, researches for new ones are developed. Some vaccinations only used experimentally are also preferred in veterinary medicine. In this study, it is aimed to examine the immunotherapeutic effect of Biocan-M (Micanfin@) vaccination.The case of our study was a 1 year old female Persan race cat. Alopecia was present in the caudal, cranial, thoracal and pectoral areas. In the anamnesis, we learned that diffuse alopecia was present and no success was obtained at the end of a 2 weeks period cure with oral antimycotic use 3 weeks before. Dermatological samples were obtained from alopecic areas in order to prepare samples with KOH (5%) in which microscopically spores were seen around the hair shaft. In culture on Sabouraud glucose agar at 25°C Microsporunz canis could be identified. Before the treatment, haemogram parameters were found normal, Zn level was low and ALT level was found high.The cure of Biocan-M (Micanfin") was divided in two stages with a 21 days break and it was supported by vitamin complexes and Zn. After the cure, Zn level was found normal, diffuse alopecia was absent and lesions were lost.It is conluded that Biocan-M (Micanfin") vaccination once a year has a strong immunotherapeutic effect.Candida species have become important among the etiologic agents of the nasopharyngeal septicemia cases. It has been assumed that 10% and 20% of the entire nosocomial septicemia cases are due to Candida species. Candidosis is a serious case with high mortality and its treatment is difficult. Thus, type classification of the Candida species detected in the blood samples of the patients was performed by PCR and DNA sequence analysis.The aim of this study is the prevalence of dermatomycosis in our clinic in the after war period.During the period of July-December 1999 9390 inand-out-patients visited our clinic. The direct microscopic examination was used.From all patients 564 had mycotic infections. From this number, 293 (51.9%) presented with superficial mycosis; 43 (7.6%) with deep hair trichophytia; 4 (0.7%) had favus; 26 (4.6%) had candidosis; in 165 (29.2%) cases pityriasis versicolor was found and 33 (5.8%) had onychomycosis.We can resume that worseness of living conditions in the after war period was favorable for spreading of dermatomycoses.The prevalence, abundance, species detected, and drug susceptibility of fungi present in stool was determined. A total of 258 children were tested: 148 with non-specific gastrointestinal tract disorders, and 1 10 asthmatics.A quantitative evaluation was performed to detect the number of fungi in one gram of stool. The number of fungi I 103/1 gram was considered non-pathognostic, 104-105-doubtful, and > 105-pat...