Type 1 fimbrial shafts of Escherichia coli and Klebsiella pneumoniae influence sugar-binding specificities of their FimH adhesins

Madison, Bereneice; Ofek, Itzhak; Clegg, Steven; Abraham, Soman N.

doi:10.1128/iai.62.3.843-848.1994

Cited by 71 publications

(32 citation statements)

References 33 publications

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“…Quantitative differences in adhesive activity may therefore be a function of ¢mbrial proteins distinct from FimH. The e¡ect of ¢mbrial proteins on the adhesive phenotype has been reported for E. coli and K. pneumoniae type 1 ¢mbriae [22]. In Salmonella strains, an interaction between FimH and FimF may be serovar-speci¢c leading to e¤cient assembly of FimH into a ¢mbrial shaft in the presence of homologous FimF.…”

Section: Discussionmentioning

confidence: 98%

Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium

Hancox¹

1997

FEMS Immunology and Medical Microbiology

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Mutations in the fimH gene of Salmonella typhimurium result in a non-fimbriate, non-adhesive phenotype. This phenotype was shown to be due to the lack of both fimH and fimF expression since disruption of the fimH gene by insertion of a DNA cassette into this determinant results in mutants that are complemented by plasmids carrying both fimH and fimF. Deletion mutations within the S. typhimurium fimH gene carried on a recombinant plasmid can be used to complement the mutant, and these transformants are non-adhesive but fully fimbriate, consistent with the role of FimH as being necessary for fimbrial adhesin expression. Adherence to erythrocytes, HeLa, and Hep-2 cells is associated with expression of the FimH polypeptide, and fimbriate strains that cannot synthesize FimH are non-adhesive. Discrete differences in the amino acid sequences of the adhesive type 1 and the non-hemagglutinating type 2 FimH polypeptides were detected, and are most likely responsible for the differences in hemagglutinating activity.

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Section: Discussionmentioning

confidence: 98%

Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium

Hancox¹

1997

FEMS Immunology and Medical Microbiology

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“…Several repetitions of monoxenic cultures of strains HM-1:IMSS and HK-9 were done. Seven separate cultures were initiated with E. coli serotype O55 (7), and four were initiated with E. coli 346, which has type I pili (24). The bacteria were grown separately in TYI-S-33 medium for 10 h at 37°C, and 1 ml of the bacterial culture was then added to the trophozoite cultures at each subculture.…”

Section: Ameba Culturesmentioning

confidence: 99%

Down Regulation ofEntamoeba histolyticaVirulence by Monoxenic Cultivation withEscherichia coliO55 Is Related to a Decrease in Expression of the Light (35-Kilodalton) Subunit of the Gal/GalNAc Lectin

et al. 1999

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Entamoeba histolytica virulence is related to a number of amebic components (lectins, cysteine proteinases, and amebapore) and host factors, such as intestinal bacterial flora. Trophozoites are selective in their interactions with bacteria, and the parasite recognition of glycoconjugates plays an important role in amebic virulence. Long-term monoxenic cultivation of pathogenic E. histolyticatrophozoites, strains HK-9 or HM-1:IMSS, with Escherichia coli serotype O55, which binds strongly to the Gal/GalNAc amebic lectin, markedly reduced the trophozoites’ adherence and cytopathic activity on cell monolayers of baby hamster kidney (BHK) cells. Specific probes prepared from E. histolytica lectin genes as well as antibodies directed against the light (35-kDa) and heavy (170-kDa) subunits of the Gal/GalNAc lectin revealed a decrease in the transcription and expression of the light subunit in trophozoites grown monoxenically with E. coli O55. This effect was not observed when E. histolyticawas grown with E. coli 346, a mannose-binding type I pilated bacteria. Our results suggest that the light subunit of the amebic lectin is involved in the modulation of parasite adherence and cytopathic activity.

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“…Type 1 fimbriae are encoded by the majority of Enterobactericeae (Duguid et al, 1955). They belong to the chaperone-usher class fimbriae family and are encoded by the fimABCDEFGHIK gene cluster, with fimA being the major structural subunit while fimH encodes for the adhesive subunit, FimH, which was shown to mediate adhesion to mannose-containing structures present on host tissue surfaces and extracellular matrix Krogfelt et al, 1990;Madison et al, 1994). Type 1 fimbrial expression is phase variable, that is, mediated by an invertible DNA element (fim switch) (Klemm & Schembri, 2000;Struve et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Biofilm formation ofKlebsiella pneumoniaeon urethral catheters requires either type 1 or type 3 fimbriae

Stahlhut

Struve

Krogfelt

et al. 2012

FEMS Immunol Med Microbiol

120

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Urinary catheters are standard medical devices utilized in both hospital and nursing home settings, but are associated with a high frequency of catheter‐associated urinary tract infections (CAUTI). In particular, biofilm formation on the catheter surface by uropathogens such as Klebsiella pneumoniae causes severe problems. Here we demonstrate that type 1 and type 3 fimbriae expressed by K. pneumoniae enhance biofilm formation on urinary catheters in a catheterized bladder model that mirrors the physico‐chemical conditions present in catheterized patients. Furthermore, we show that both fimbrial types are able to functionally compensate for each other during biofilm formation on urinary catheters. In situ monitoring of fimbrial expression revealed that neither of the two fimbrial types is expressed when cells are grown planktonically. Interestingly, during biofilm formation on catheters, both fimbrial types are expressed, suggesting that they are both important in promoting biofilm formation on catheters. Additionally, transformed into and expressed by a nonfimbriated Escherichia coli strain, both fimbrial types significantly increased biofilm formation on catheters compared with the wild‐type E. coli strain. The widespread occurrence of the two fimbrial types in different species of pathogenic bacteria stresses the need for further assessment of their role during urinary tract infections.

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Type 1 fimbrial shafts of Escherichia coli and Klebsiella pneumoniae influence sugar-binding specificities of their FimH adhesins

Cited by 71 publications

References 33 publications

Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium

Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium

Down Regulation ofEntamoeba histolyticaVirulence by Monoxenic Cultivation withEscherichia coliO55 Is Related to a Decrease in Expression of the Light (35-Kilodalton) Subunit of the Gal/GalNAc Lectin

Biofilm formation ofKlebsiella pneumoniaeon urethral catheters requires either type 1 or type 3 fimbriae

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