Purposes: The purposes of this review were to compare sensitivity and specificity of the new generation tympanic thermometry under different cutoffs and to give the optimal cutoff. Methods: Articles were derived from a systematic search in PubMed, Web of science, and Embase, and were assessed for internal validity by QUADAS-Ⅱ. The figure of risk of bias was drew by Review Manager 5.3 and data were synthesized by MetaDisc 1.4. Results: Nineteen diagnostic studies, involving 4,639 pediatric patients, were included. The cut-offs varied from 37.0℃ to 38.0℃ among these studies. The cut-off 37.8 was with the highest sROC AUC (0.97) and Youden Index (0.83) and was deemed to be the optimal cutoff. Conclusion: New Generation Tympanic Thermometry is with high diagnostic accuracy in pediatric patients and can be an alternative for rectal measurements.