Two γ-polypeptides of B-phycoerythrin from Porphyridium cruentum

Stadnichuk, Igor N.; Karapetyan, N. V.; Kislov, L.D.; Semenenko, V. E.; Veryasov, M.B.

doi:10.1016/s1011-1344(97)83321-0

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…These should be proved by reverse-phase high-pressure liquid chromatography as described by Swanson and Glazer (1990) and Stadnichuk et al (1993). The k-subunits of red algae generally carry 4 or 5 phycobilins (PUB and/or PEB) and the number of k-subunits varies within species (Stadnichuk 1995, Stadnichuk et al 1997, Ritz et al 1998, Talarico et al 1998. Only a single k-subunit was identified in R-PE from P. palmata (Galland-Irmouli et al 2000).…”

Section: The Three -Subunitsmentioning

confidence: 99%

Two forms of phycobilisomes in the Antarctic red macroalga Palmaria decipiens (Palmariales, Florideophyceae)

Lüder

Knoetzel

Wiencke

2001

Physiologia Plantarum

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The phycobilisomes (PBS), the light-harvesting antennae, from the endemic Antarctic red macroalga Palmaria decipiens were isolated on discontinuous sucrose gradients in two discrete bands and not in one as expected. To exclude methodical faults, we also isolated PBS from the temperate Palmaria palmata and the unicellular red algae Porphyridium cruentum and Rhodella violacea. In P. palmata the PBS were separated in two discrete bands, whereas the PBS from Porphyridium and Rhodella were found in one band. The double-banded PBS (PBSup and PBSlow) from P. decipiens were further characterized by absorption and fluorescence spectroscopy, native and SDS-PAGE as well as by negative staining. The phycobiliproteins RIII-phycoerythrin, RI-phycocyanin and allophycocyanin were identified and 3 gamma-subunits were described. The PBSup and PBSlow showed no significant differences in their absorption spectra and phycobiliprotein ratios although the negative stained PBSlow were smaller. Differences were found in their low molecular mass subunit complexes, which are assumed to be r-phycoerythrin. The polypeptide pattern of the PBSup and PBSlow showed no differences in the molecular masses of their subunits and linker polypeptides, but in their percentage distribution. The results suggest that the PBSlow is a closer packed and PBSup a little more loosely aggregated hemiellipsiodal PBS form. We discuss the ecophysiological function of two PBS forms in P. decipiens and suggest advantages in the rapid acclimation to changes in environmental light conditions.

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Section: The Three -Subunitsmentioning

confidence: 99%

Two forms of phycobilisomes in the Antarctic red macroalga Palmaria decipiens (Palmariales, Florideophyceae)

Lüder

Knoetzel

Wiencke

2001

Physiologia Plantarum

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“…The phycobilin number in a g subunit has been reported to be one, 16 four 1,17,18 or even five 19 for various phycoerythrins. To our knowledge, this is the first time that two types of g subunits of R-PE from Polysiphonia urceolata have been obtained.…”

Section: Resultsmentioning

confidence: 99%

“…First of all, during the separation and renaturation, never had a reagent been used to break the covalent bonds so as to cut one chromophore off, in addition, g 2 should not be a fragment of g 1 for it possesses a larger molecular weight but less chromophores. Secondly, it has been reported for various red algae that R-PE and B-PE could have two or three g subunits, 19 therefore, it is reasonable for R-PE to have more than one g subunit. Thirdly, the failure to determine the entire structures of the g subunits in R-PE, b-or B-PE by X-ray crystal structure analyses, which has been ascribed to different orientations of the g subunit in the central channel of the R-PE, 5 may also be evidence for existence of various types of g subunits.…”

Section: Discussionmentioning

confidence: 98%