Two Zinc Finger Proteins, OMA-1 and OMA-2, Are Redundantly Required for Oocyte Maturation in C. elegans

Detwiler, Michelle R.; Reuben, Melanie; Li, Xiumin; Rogers, Eric; Lin, Rueyling

doi:10.1016/s1534-5807(01)00026-0

Cited by 165 publications

(238 citation statements)

References 52 publications

(15 reference statements)

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“…Along with looking at the mere presence of sperm, we examined three strains in which both sperm and oocytes are present but their interactions are impaired. In the first strain, the oma-1;oma-2 double mutant, ovulation never occurs and so the two types of gametes never meet (22). The production of pheromone by the oma-1;oma-2 double mutant indicates that the presence of normal sperm is insufficient to suppress pheromone production.…”

Section: Significancementioning

confidence: 99%

Communication between oocytes and somatic cells regulates volatile pheromone production in Caenorhabditis elegans

Leighton

Choe

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Males of the androdioecious species Caenorhabditis elegans are more likely to attempt to mate with and successfully inseminate C. elegans hermaphrodites that do not concurrently harbor sperm. Although a small number of genes have been implicated in this effect, the mechanism by which it arises remains unknown. In the context of the battle of the sexes, it is also unknown whether this effect is to the benefit of the male, the hermaphrodite, or both. We report that successful contact between mature sperm and oocyte in the C. elegans gonad at the start of fertilization causes the oocyte to release a signal that is transmitted to somatic cells in its mother, with the ultimate effect of reducing her attractiveness to males. Changes in hermaphrodite attractiveness are tied to the production of a volatile pheromone, the first such pheromone described in C. elegans.fertilization | sex pheromones | egg-soma communication I ts properties of self-fertilization and rapid generation, along with its extensive library of mutants, make Caenorhabditis elegans an excellent system in which to study reproductive events. The generation time of C. elegans is under 3 d, and a single hermaphroditic worm can use its sperm to fertilize its own eggs, without the need for mating (1). C. elegans and related nematodes have a robust sperm sensation pathway that limits unfruitful oocyte maturation and ovulation (2). Both self-sperm and nonself-sperm secrete protein ligands, known as major sperm proteins (MSPs), that activate signal transduction pathways in both unfertilized oocytes, leading to activation of mitogen-activated protein kinase (MPK-1), and the somatic gonad, involving transcription factor CEH-18. The result of this signaling is the release of oocytes from prophase I arrest and the ovulation of unarrested oocytes into the uterus (3, 4).Several behaviors of female and hermaphroditic nematodes have been demonstrated to correlate with either the presence of sperm or the recentness of mating. C. elegans hermaphrodites that have exhausted their supply of self-sperm are more likely to elicit a mating response from males of their species, and less likely to resist an attempted mating. Mutant C. elegans hermaphrodites that develop without self-sperm also elicit more mating attempts, and this increase in attractiveness vanishes after a successful mating (5, 6). In the gonochoristic species Caenorhabditis brenneri and Caenorhabditis remanei, males are attracted to a volatile pheromone produced only by females that have not recently mated (7). The mechanisms that link these behaviors to sperm status remain unknown.Pheromones have been shown to exist in dozens of nematode species (8-10) and have been positively identified in several, including C. elegans (11-14). Although HPLC-MS studies have led to the identification of more than 140 pheromones and pheromone-related metabolites in C. elegans (15), little is known about how production of such pheromones is regulated. Life stage and environmental conditions have been shown to affect pheromone o...

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Section: Significancementioning

confidence: 99%

Communication between oocytes and somatic cells regulates volatile pheromone production in Caenorhabditis elegans

Leighton

Choe

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…MEX-3 expression is inverse to Type B, being abundant in the mitotic region, very low in early prophase, but abundant again in late prophase (Type C) (Mootz et al 2004 ;Ciosk et al 2006 ) . PUF-5 and the zinc fi nger proteins OMA-1 and OMA-2 are restricted to meiotic stages beyond the germline loop, corresponding to the last two meiotic prophase stages, diplotene and diakinesis (Type D) (Detwiler et al 2001 ;Lublin and Evans 2007 ) .…”

Section: Expression and Activity Domains Of Rna Regulatorsmentioning

confidence: 99%

Translational Control in the Caenorhabditis elegans Germ Line

Nousch

Eckmann

2012

Advances in Experimental Medicine and Biology

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Translational control is a prevalent form of gene expression regulation in the Caenorhabditis elegans germ line. Linking the amount of protein synthesis to mRNA quantity and translational accessibility in the cell cytoplasm provides unique advantages over DNA-based controls for developing germ cells. This mode of gene expression is especially exploited in germ cell fate decisions and during oogenesis, when the developing oocytes stockpile hundreds of different mRNAs required for early embryogenesis. Consequently, a dense web of RNA regulators, consisting of diverse RNA-binding proteins and RNA-modifying enzymes, control the translatability of entire mRNA expression programs. These RNA regulatory networks are tightly coupled to germ cell developmental progression and are themselves under translational control. The underlying molecular mechanisms and RNA codes embedded in the mRNA molecules are beginning to be understood. Hence, the C. elegans germ line offers fertile grounds for discovering post-transcriptional mRNA regulatory mechanisms and emerges as great model for a systems level understanding of translational control during development.

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“…The known regulatory interactions are derived from the observation that oma-1;oma-2 RNAi embryos have missegregation of PGL-1, PIE-1 and MEX-1 in the early embryo, 33 and that a gain-of-function oma-1 mutant has delayed degradation of maternal proteins including SKN-1, PIE-1, MEX-3 and MEX-5. 34 From the information we currently have, the most likely regulators of the module are OMA-1 and its paralog OMA-2, which have a reported function in oocyte maturation, 35 and ZIM-2, which is required for segregation of chromosomes during meiosis; 36 both are processes that are in accordance with the GO enrichment of the module genes.…”

Section: Meiosis and Oogenesismentioning

confidence: 95%

Computational and Systems Biology

Babu¹,

Mori²

2009

Mol. BioSyst.

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Over the last few decades, we have all witnessed an explosion in the amount and diversity of information that describes various living systems, including humans. This ranges from the availability of the complete genome sequence of over a thousand organisms to datasets describing expression levels of thousands of transcripts across different conditions to networks of interactions between different biological molecules. Thus it is clear that we are now in an exciting time in biology where the availability of such data permits us to investigate fundamental questions of a different magnitude and kind that are complementary to conventional approaches. This diversity in problems that one can currently address is well illustrated by the articles published in this themed issue of Molecular BioSystems entitled ''Computational and Systems Biology''. The excitement in this area and the scientific community is reflected in the fact that this special issue consists of over 50 papers with 19 reviews and 32 original research articles.

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Two Zinc Finger Proteins, OMA-1 and OMA-2, Are Redundantly Required for Oocyte Maturation in C. elegans

Cited by 165 publications

References 52 publications

Communication between oocytes and somatic cells regulates volatile pheromone production in Caenorhabditis elegans

Communication between oocytes and somatic cells regulates volatile pheromone production in Caenorhabditis elegans

Translational Control in the Caenorhabditis elegans Germ Line

Computational and Systems Biology

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