Two-way antigenic cross-reactivity between porcine epidemic diarrhea virus and porcine deltacoronavirus

Ma, Yuanmei; Liang, Xueya; Oglesbee, Michael; Krakowka, Steven; Niehaus, Andrew J.; Wang, Guiping; Jia, Aiqing; Song, Houhui; Lǐ, Jiànróng

doi:10.1016/j.vetmic.2016.02.004

Cited by 39 publications

(46 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the eukaryotic-expressed S1 protein is likely more advantageous than the WV as the antigen for PEDV serological assays. For another two major structural proteins M and N, due to common epitopes shared by PEDV, TGEV and PDCoV, several studies have previously demonstrated that PEDV M or N presented some cross-reactivity to TGEV or PDCoV (Gimenez-Lirola et al, 2017;Lin et al, 2015;Ma et al, 2016). In contrast, recombinant PEDV-S1 had no cross-reactivity with sera from these porcine coronaviruses, showing the best diagnostic sensitivity (Gimenez-Lirola et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers

Lei

Yang

et al. 2019

Veterinary Microbiology

View full text Add to dashboard Cite

A B S T R A C TGiven the highly contagious and acute nature of porcine epidemic diarrhea (PED), especially in piglets, there is an urgent need for the development of rapid and sensitive diagnostic assays. The diagnostic potentials of specific porcine epidemic diarrhea virus (PEDV) accessory and nonstructural proteins, if any, have not yet been investigated. In order to determine and compare which of the viral proteins may be useful as diagnostic antigens, whole virus (WV) particles and a panel of structural and nonstructural PEDV proteins [spike subunit 1 (S1), the C-terminal part of ORF3 (ORF3C), envelope (E), nonstructural protein 1 (Nsp1), Nsp2, Ac (acidic domain of Nsp3), and ADRP (ADP-ribose-1-monophosphatase domain of Nsp3), expressed individually in bacterial and/or mammalian cells] were tested for reactivity with sera from PEDV-infected pigs by ELISA and/or western blot analysis. According to western blots, serum antibody interactions with the S1 protein were relatively more sensitive and specific than ORF3C, E and Ac. Furthermore, a total of 851 serum samples from diarrheal pigs of different ages were analyzed by ELISA, with most showing immune-reactivity towards the WV, S1, ORF3C, and E proteins. The earliest IgG antibody response was observed in the one-week-old piglets, with similar antibody ontogeny and patterns of seroconversion for S1, ORF3C, E, and WV antigens. In addition, the pattern of neutralizing antibody was more similar to that of IgA in weaning piglets after PEDV infection. Collectively, these data provide more reliable information on the host immune response to different viral proteins, which will be useful for development of novel serological assays and for design of vaccines that better stimulate protective immunity.

show abstract

Section: Discussionmentioning

confidence: 99%

Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers

Lei

Yang

et al. 2019

Veterinary Microbiology

View full text Add to dashboard Cite

show abstract

“…Although several standard detection methods, for example virus isolation, virus neutralization tests, and indirect immunofluorescence assay, are available for the detection of viruses, these techniques are time‐consuming and not suitable for detecting large‐scale samples. Currently, polymerase chain reaction (PCR) and enzyme‐linked immunosorbent assay (ELISA) methods for the detection of these viruses have been reported (Luo et al, ; Ma et al, ; Zhu, Wang, Cui, & Cui, ). Due to the high pathogenicity of these viruses to suckling piglets, which have immature immune systems and few antibodies, ELISA is inefficient for detecting these viruses compared to PCR.…”

Section: Introductionmentioning

confidence: 99%

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

Ding

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

The major enteric RNA viruses in pigs include porcine epidemic diarrhoea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus A (PRV‐A), porcine kobuvirus (PKV), porcine sapovirus (PSaV) and porcine deltacoronavirus (PDCoV). For differential diagnosis, a multiplex RT‐PCR method was established on the basis of the N genes of TGEV, PEDV and PDCoV, the VP7 gene of PRV‐A, and the polyprotein genes of PKV and PSaV. This multiplex RT‐PCR could specifically detect TGEV, PEDV, PDCoV, PRV‐A, PKV and PSaV without cross‐reaction to any other major viruses circulating in Chinese pig farms. The limit of detection of this method was as low as 100–101 ng cDNA of each virus. A total of 398 swine faecal samples collected from nine provinces of China between October 2015 and April 2017 were analysed by this established multiplex RT‐PCR. The results demonstrated that PDCoV (144/398), PSaV (114/398), PEDV (78/398) and PRV‐A (70/398) were the main pathogens, but TGEV was not found in the pig herds in China. In addition, dual infections, for example, PDCoV + PSaV, PDCoV + PRV‐A, PRA‐V + PSaV and PEDV + PDCoV, and triple infections, for example, PDCoV + PRV‐A + PSaV and PEDV + PDCoV + PKV, were found among the collected samples. The multiplex RT‐PCR provided a valuable tool for the differential diagnosis of swine enteric viruses circulating in Chinese pig farms and will facilitate the prevention and control of swine diarrhoea in China.

show abstract

“…In fact, recently antigenic cross-reactivity between PDCoV Michigan strain and PEDV, mediated by N protein, has been detected using a monoclonal antibody specific for N protein. Nevertheless, no cross-neutralization has been detected between these strains (Ma et al, 2016).…”

Section: Porcine Delta Coronavirus (Pdcov)mentioning

confidence: 92%

“…Chinese ferret-badger that were positive in 2.4% of and 1.1% of the animals tested, respectively Ma et al, 2015;Wang et al, 2014a).Phylogenetic analysis of PDCoV S gene nucleotide sequence illustrates the close relationship between PDCoV, delta-CoVs of small mammals, avian-delta CoVs, and alpha-CoVs (Fig. 7) (Hu et al, 2015).…”

Section: Porcine Delta Coronavirus (Pdcov)mentioning

confidence: 95%

See 1 more Smart Citation

Porcine gastroenteric viruses: pathogenesis and protection

Enjuanes¹,

Pascual²,

Sánchez³

et al. 2016

Vetkorm

View full text Add to dashboard Cite

Two-way antigenic cross-reactivity between porcine epidemic diarrhea virus and porcine deltacoronavirus

Cited by 39 publications

References 16 publications

Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers

Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

Porcine gastroenteric viruses: pathogenesis and protection

Contact Info

Product

Resources

About