Two-voxel-analysis to assess the spatial resolution of a tracer in Lissajous-scanning magnetic particle imaging

Kosch, Olaf; Remmo, Amani; Wells, James; Wiekhorst, Frank

doi:10.1016/j.jmmm.2022.170170

Cited by 3 publications

(5 citation statements)

References 15 publications

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“…To assess the spatial resolution of the three MNP systems SynP50, SynC30, and RES in cells, we conducted the two-voxel analysis as described previously 44 . Instead of using a reference sample prepared directly from MNP stock suspension, 5·10 6 MNP-loaded cells were used for SF acquisition.…”

Section: Resultsmentioning

confidence: 99%

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Counting cells in motion by quantitative real-time magnetic particle imaging

Remmo,

Kosch,

Kampen

et al. 2024

Sci Rep

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Magnetic Particle Imaging (MPI) is an advanced and powerful imaging modality for visualization and quantitative real-time detection of magnetic nanoparticles (MNPs). This opens the possibility of tracking cells in vivo once they have been loaded by MNPs. Imaging modalities such as optical imaging, X-ray computed tomography (CT), positron emission tomography (PET), single photon emission computed tomography (SPECT), and magnetic resonance imaging (MRI) face limitations, from depth of penetration and radiation exposure to resolution and quantification accuracy. MPI addresses these challenges, enabling radiation-free tracking of MNP-loaded cells with precise quantification. However, the real-time tracking of MNP-loaded cells with MPI has not been demonstrated yet. This study establishes real-time quantitative tracking of MNP-loaded cells. Therefore, THP-1 monocytes were loaded with three different MNP systems, including the MPI gold standard Resovist and Synomag. The real-time MPI experiments reveal different MPI resolution behaviors of the three MNP systems after cellular uptake. Real-time quantitative imaging was achieved by time-resolved cell number determination and comparison with the number of inserted cells. About 95% of the inserted cells were successfully tracked in a controlled phantom environment. These results underline the potential of MPI for real-time investigation of cell migration and interaction with tissue in vivo.

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Section: Resultsmentioning

confidence: 99%

“…Figure 3 illustrates the separation quality Q s as a function of distance x (number of empty voxels) as obtained by the two-voxel analyzes for samples containing MNP-loaded cells from which the MPI resolution r is extracted 44 . The resolution r is defined as the voxel distance x at which Q s is 50%.…”

Section: Resultsmentioning

confidence: 99%

Counting cells in motion by quantitative real-time magnetic particle imaging

Remmo,

Kosch,

Kampen

et al. 2024

Sci Rep

Self Cite

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show abstract

“…The two-voxel-analysis is an efficient procedure to determine the achievable resolution in MPI directly from a SF measurement to synthetically construct MPI measurements of two point-like tracer samples at varying distances. 20 To simulate an array of two different tracers sources, the corresponding data are extracted from the SF and superimposed, with the additional inclusion of noise data from an empty scanner measurement, giving the total complex voltage signal ū . We only used frequency components of the SF above a certain signal-to-noise (SNR > 4) level, otherwise the reconstruction of the tracer distribution would be based on the noise of the SF acquisition.…”

Section: Methodsmentioning

confidence: 99%

“…The result is normalized to the iron content of the voxel. 20 The image resolution r is then determined by the distance at which the minimum has decreased to 50% of the maximum tracer content. To compare the resolution of different tracer systems, we used a spiral resolution phantom as introduced in ref.…”

Section: Methodsmentioning

confidence: 99%

“…For nine different tracer systems, we first correlated characteristic MPS parameters as well as the hydrodynamic diameter and size distribution extracted from dynamic light scattering (DLS) to the resolution r obtained by the recently introduced two-voxel-analysis of MPI SFs. 20 To demonstrate the performance of our analysis we compared the results with phantom measurements of a spiral phantom developed for MPI resolution measurements. 21 …”

Section: Introductionmentioning

confidence: 99%

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