Two Vibrio splendidus related strains collaborate to kill Crassostrea gigas: taxonomy and host alterations

Renault, Tristan; Pons, Anne-Marie; Roux, Frédérique Le

doi:10.3354/dao062065

Cited by 122 publications

(133 citation statements)

References 60 publications

(55 reference statements)

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“…Then, 16 oysters were challenged by injection in the adductor muscle of 100 l of Vibrio tasmaniensis LGP32 (1 ϫ 10 7 CFU/oyster), an oyster pathogen (22) recently assigned to V. tasmaniensis within the Splendidus clade (23). After 24 h, gills were dissected, frozen at Ϫ80°C, and ground to fine powder.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Histones and DNA Traps in Invertebrate Immunity

Poirier

Schmitt

Rosa

et al. 2014

Journal of Biological Chemistry

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Background: How antimicrobial histones participate in invertebrate defense was still unclear. Results: Upon injury or infection, oyster immune cells release antimicrobial histones and extracellular DNA traps in a ROSdependent manner. Conclusion: DNA traps are involved in the defense of Lophotrochozoa. Their mechanistic bases are shared with vertebrates. Significance: This is a novel mechanism in the evolutionary conserved invertebrate immune arsenal.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial Histones and DNA Traps in Invertebrate Immunity

Poirier

Schmitt

Rosa

et al. 2014

Journal of Biological Chemistry

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“…However, up to now, little has been known about the route of infection and pathogenic processes of LGP32 (2,3). A metalloprotease has been associated with toxicity (4,5) and the outer membrane protein (OMP) OmpU was shown to be a major determinant of LGP32 virulence (6).…”

mentioning

confidence: 99%

Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus

Duperthuy

Schmitt

Garzón

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

152

168

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OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen Vibrio cholera, require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen Vibrio splendidus LGP32. Here, we showed that V. splendidus LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin/invasin required for β-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase CgEcSOD, is used as an opsonin mediating the OmpU-promoted phagocytosis through its RGD sequence. Finally, the endocytosed bacteria were found to survive intracellularly, evading the host defense by preventing acidic vacuole formation and limiting reactive oxygen species production. We conclude that (i) V. splendidus is a facultative intracellular pathogen that manipulates host defense mechanisms to enter and survive in host immune cells, and (ii) that OmpU is a major determinant of host cell invasion in Vibrio species, used by V. splendidus LGP32 to attach and invade oyster hemocytes through opsonisation by the oyster plasma Cg-EcSOD.T he oyster pathogen, Vibrio splendidus strain LGP32 was isolated from massive mortality events in the production of Crassostrea gigas oysters (1). However, up to now, little has been known about the route of infection and pathogenic processes of LGP32 (2, 3). A metalloprotease has been associated with toxicity (4, 5) and the outer membrane protein (OMP) OmpU was shown to be a major determinant of LGP32 virulence (6).As bacterial surface components, OMPs are both used by hosts for pathogen recognition and by pathogens for interaction with and invasion of host cells, serving as adhesion proteins (adhesins)

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“…Among these, the Splendidus clade contains the highest number of species (.10), which have been found to be the dominant Vibrio species in coastal marine sediments, seawater and bivalves in temperate climates (Lambert et al, 1998;Sobecky et al, 1998). Some of these species have been associated with mortality of a wide range of marine animals such as molluscs, crustaceans and fish (Kueh & Chan, 1985;Pujalte et al, 1993;Nicolas et al, 1996;Leano et al, 1998;Sugumar et al, 1998;Lacoste et al, 2001;Le Roux et al, 2002;Waechter et al, 2002;Farto et al, 2003;Jensen et al, 2003;Gay et al, 2004;Gó mez-Leó n et al, 2005).…”

Section: T (5cect 7226mentioning

confidence: 99%