Two Urosoma species (Ciliophora, Hypotrichia): A multidisciplinary approach provides new insights into their ultrastructure and systematics

Dong, Jingyi; Li, Lifang; Fan, Xinpeng; Ma, Hongyu; Warren, Alan

doi:10.1016/j.ejop.2019.125661

Cited by 33 publications

(27 citation statements)

References 91 publications

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“…They are close to the contractile vacuole and are morphologically similar to the slit‐like contractile vacuole pores in Cotterillia bromelicola , Metopus murrayensis , Urosoma emarginata , and Urosoma salmastra (Dong et al. ; Foissner and Stoeck ; Vďačný and Foissner ). Therefore, these two slits might represent the contractile vacuole pores of Parabistichella variabilis .…”

Section: Discussionmentioning

confidence: 78%

“…Although it has been reported in some species of hypotrich ciliates (Bardele ; Carey and Tatchell ; Dong et al. ; Foissner et al. ; Wirnsberger‐Aescht et al.…”

Section: Discussionmentioning

confidence: 97%

“…); (4) prolate elliptical extrusomes, which probably represent a kind of mucocyst, for example, in Urosoma emarginata (Dong et al. ); and (5) cup‐shaped mucocysts, which comprise a globular electron‐lucent cavity while the rest of the structure is composed of very electron‐dense material, for example, in Oxytricha granulifera and Architricha indica (Tang et al. ; Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

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An Integrative Investigation of Parabistichella variabilis (Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny

Dong

Chen

Liu

et al. 2020

J Eukaryotic Microbiology

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Hypotrichs are a highly differentiated and very diverse group of ciliated protists. Their systematics and taxonomy are challenging and call for detailed investigations on their general morphology, ultrastructure, ontogenesis, and molecular phylogeny. Here, a comprehensive study is conducted on a brackish water population of Parabistichella variabilis using light and electron microscopy and phylogenetic analyses based on small subunit ribosomal DNA sequence data. Its morphology, including the infraciliature, pellicle, nuclei, buccal seal, and extrusomes, is documented. The present findings indicate that in P. variabilis: (i) the cortical granules are extrusomes, which differ from those of other hypotrichs; (ii) the buccal seal is bounded by the plasma membrane and contains a single layer of longitudinal microtubules; (iii) two contractile vacuoles might be present rather than one; and (iv) the pharyngeal disks are bounded by a single membrane. Early‐to‐middle stages of ontogenesis are described for the first time, enabling the complete characterization of this process. Phylogenetic analyses indicate that Parabistichella variabilis is closely related to several species from different genera, such as Orthoamphisiella breviseries, Uroleptoides magnigranulosus, and Tachysoma pellionellum. However, ultrastructural and gene sequence data for more taxa are needed in order to resolve the systematics of Parabistichella.

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Section: Discussionmentioning

confidence: 78%

“…Although it has been reported in some species of hypotrich ciliates (Bardele ; Carey and Tatchell ; Dong et al. ; Foissner et al. ; Wirnsberger‐Aescht et al.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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An Integrative Investigation of Parabistichella variabilis (Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny

Dong

Chen

Liu

et al. 2020

J Eukaryotic Microbiology

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“…Additionally, Bistichella falls into a clade which includes Uroleptoides, Parabistichella, Orthoamphisiella, Lamtostyla, Keronopsis, Paraholosticha and Pseudokahliella, and shares the same morphological features, i.e., at least one long or moderate long frontoventral row, one marginal cirral row on each side, constantly three bipolar dorsal kineties, and a lack of dorsomarginal kineties. They are all non-dorsomarginalian hypotrichs, and this clade is separate with Dorsomarginalia, all species of which have dorsomarginal row [1,19,21,[23][24][25][26][27][28][29]. The result further proves the importance of dorsal structure in hypotrichous classi cation.…”

Section: Morphogenetic Comparisonmentioning

confidence: 68%

Morphology, morphogenesis and phylogeny of the new soil ciliate, Bistichella granulifera n. sp. (Protista, Ciliophora)

Lyu¹,

Wang

et al. 2020

Preprint

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Background: Hypotrichous ciliates are showing extremely morphological diverse and complicated morphogenesis. However, many hypotrichs are still unknown/poorly-known and need to be explored. Recently, Foissner (2016) reassigned the species in Bistichella and Parabistichella. Due to lack of morphological and morphogenetic information, some problems were overlooked. In present work, a new species, Bistichella granulifera n. sp., was found in northern China. Based on its infraciliature, ontogenesis analyses and phylogenetic study, the phylogenetic position of Bistichella was discussed.Results: Bistichella granulifera n. sp. was characterized by having elongated oval body, two macronuclear nodules, cortical granules present, three frontal and three or four buccal cirri, three frontal rows with seven to ten cirri, usually two frontoventral rows with the left one usually terminates at 90% down length of body and no breaks in the right one. The main morphogenetic features of the novel species were as follows: (1) the posterior part of the old adoral membranelles is renewed; (2) frontoventral-transverse cirral anlagen III to V each form a frontal row, and anlagen VI to n each produce a frontoventral row; (3) both marginal rows and dorsal kineties develop intrakinetally; (4) the macronuclear nodules fuse to form a single mass. The gene sequence of Bistichella was investigated for the first time. Phylogenetic analyses based on the SSU rDNA sequences showed that the Bistichella groups with the non-dorsomarginalian hypotrichs.Conclusions: Morphology, morphogenesis and phylogenetic position of Bistichella granulifera n. sp., was investigated. Each frontoventral row originates from one anlage and only three bipolar dorsal kineties are present in the new species, which means it should belong to non-Dorsomarginalia rather than Amphisiellidae. Even though Bistichella has a close relationship with amphisiellids, due to the limited gene sequence, the phylogenetic position of Bistichella needs more data to be fully understood.

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“…The Hypotricha Stein, 1859 is a speciose and morphologically diverse group that inhabits a wide range of biotopes [1][2][3][4][5][6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Morphology, Ontogeny and Molecular Phylogeny of a New Urostylid Ciliate, Bakuella (Pseudobakuella) Guangdongica n. sp. (Protista, Ciliophora, Hypotrichia) from Southern China

Wang

et al. 2020

Preprint

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Background: Spirotrich ciliates are one of the most diverse groupsin the phylum Ciliophora and are widely distributed in marine, freshwater, and terrestrial biotopes. Many nominal species are, however, poorly known and their systematic positions remain uncertain due to the lack of information concerning their infraciliature, morphogenesis, and gene sequences. In this paper, the morphology and morphogenesis of Bakuella (Pseudobakuella) guangdongica n. sp. were studied, in addition, genomic DNA was extracted in order to sequence the small subunit rDNA. Results: Bakuella (Pseudobakuella) guangdongica n. sp. is characterized by 150–225 µm in vivo; 35–42 adoral membranelles; three to five buccal, two frontoterminal and eight to 13 transverse + pretransverse cirri; midventral complex comprised of 10–20 midventral pairs and two midventral rows extending to transverse cirri; posterior part of marginal rows slightly overlapped; colorless cortical granules about 1 μm across, arranged in small groups; soil habitat. Its main ontogenetic features are: (1) in the proter, the parental adoral zone of membranelles is completely renewed by new structures; (2) in the opisthe, the oral primordium originates apokinetally, some old midventral cirri join the formation of frontoventral-transverse cirral anlagen; (3) the anlagen for marginal rows and dorsal kineties develop intrakinetally; and (4) the numerous macronuclear nodules fuse into a single mass before dividing. Phylogenetic analyses based on the SSU rDNA sequence suggests the non-monophyly of the genus Bakuella. Conclusions: The morphology, morphogenesis and molecular phylogeny of the new hypotrichous ciliate, Bakuella (Pseudobakuella) guangdongica n. sp. were studied, the phylogenetic analyses show that the Bakuella is polyphyletic in the SSU rDNA.

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Two Urosoma species (Ciliophora, Hypotrichia): A multidisciplinary approach provides new insights into their ultrastructure and systematics

Cited by 33 publications

References 91 publications

An Integrative Investigation of Parabistichella variabilis (Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny

An Integrative Investigation of Parabistichella variabilis (Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny

Morphology, morphogenesis and phylogeny of the new soil ciliate, Bistichella granulifera n. sp. (Protista, Ciliophora)

Morphology, Ontogeny and Molecular Phylogeny of a New Urostylid Ciliate, Bakuella (Pseudobakuella) Guangdongica n. sp. (Protista, Ciliophora, Hypotrichia) from Southern China

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