Two types of squamous cell carcinoma of the palatine tonsil characterized by distinct etiology, molecular features and outcome

Charfi, Lamia; Jouffroy, Thomas; Crémoux, Patricia de; Peltier, Nelly Le; Thioux, Martine; Fréneaux, Paul; Point, D; Girod, Angélique; Rodriguez, José; Sastre-Garau, Xavier

doi:10.1016/j.canlet.2007.10.028

Cited by 64 publications

(47 citation statements)

References 29 publications

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“…It is widely known that tobacco and alcohol consumption are the major causes of the development of oral squamous cell carcinoma (OSCC) (2). The fact that 15-20% of patients develop OSCC in the absence of exposure to these agents, however, strongly suggests the existence of other risk factors in oral carcinogenesis, such as the presence of infectious agents (2).…”

Section: Discussionmentioning

confidence: 99%

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Incidence of Low Risk Human Papillomavirus in Oral Cancer: A Real Time PCR Study on 278 Patients

Palmieri

Scapoli

Martinelli

et al. 2011

Int J Immunopathol Pharmacol

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Squamous cell carcinoma is the most frequent malignant tumour of the oral cavity. It is widely known that tobacco and alcohol consumption are the major causes of the development of oral squamous cell carcinoma (OSCC). The human papilloma virus infection has also been postulated as a risk factor for squamous cell carcinoma, although conflicting results have been reported. The aim of this study is to evaluate the presence of high-risk and low-risk type human papillomavirus in a large sample of squamous cell carcinoma limited to the oral cavity by means of quantitative real-time polymerase chain reaction. Data were obtained from 278 squamous cell carcinoma limited to oral cavity proper. Sequencing revealed that 5 samples were positive for HPV type 16, 5 for HPV type 11, and 1 for HPV type 6. Human papillomavirus 11 was detected in 5 tumours out of the 278 examined. The prevalence rate for Human papillomavirus 11 was 1.8% (C.I. 0.7–3.9). The matched case-controls analysis indicated that the prevalence among controls did not significantly differ with respect to cases and that Human papillomavirus 11 alone did not correlate with squamous cell carcinoma.

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Section: Discussionmentioning

confidence: 99%

“…The most important risk factors for this cancer are certainly the consumption of alcohol and tobacco, although other factors such as genetic susceptibility, diet and infection by viral agents appear to playa synergic role in the development of the disease (2).…”

mentioning

confidence: 99%

Incidence of Low Risk Human Papillomavirus in Oral Cancer: A Real Time PCR Study on 278 Patients

Palmieri

Scapoli

Martinelli

et al. 2011

Int J Immunopathol Pharmacol

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“…Most researchers agree that nuclear p16 expression is required, however, the cut-off for nuclear expression varies between studies and has been as low as 5%. The sensitivity and specificity for nuclear tumor p16 expression as a surrogate biomarker for HPV is 69% (95% CI 61%, 75%) and 90% (95% CI 81%, 95%) [51,[61][62][63][64]. As patients with HPV-mediated cancers have a better prognosis, are they being over-treated by current protocols?…”

Section: Human Papillomavirusmentioning

confidence: 99%

Prognostic Indicators in Head and Neck Oncology Including the New 7th Edition of the AJCC Staging System

Brandwein-Gensler

Smith

2010

Head and Neck Pathol

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“…Recently, several studies have shown that HPV are clearly involved in the pathogenesis of a subgroup of OSCC and OPSCC [1,2]. This distinct subgroup of Head and Neck Cancers is characterized by distinctive histopathological features: HPV infection, distinctive epidemiology, better response to induction chemotherapy and concurrent chemo-radiation protocol and an overall better clinical outcome, as compared to HPV negative HN-SCC [3][4][5][6]. The proportion of OPSCCs that are potentially HPV-related (cancers of the tongue-based and tonsils, including lingual tonsil and Waldeyer's ring) increased in the USA from 1973 to 2004, perhaps as a result of changing sexual behaviours.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a combined triple method to detect causative HPV in oral and oropharyngeal squamous cell carcinomas: p16 Immunohistochemistry, Consensus PCR HPV-DNA, and In Situ Hybridization

Pannone

Rodolico

Santoro

et al. 2012

Infectious Agents and Cancer

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Background: Recent emerging evidences identify Human Papillomavirus (HPV) related Head and Neck squamous cell carcinomas (HN-SCCs) as a separate subgroup among Head and Neck Cancers with different epidemiology, histopathological characteristics, therapeutic response to chemo-radiation treatment and clinical outcome. However, there is not a worldwide consensus on the methods to be used in clinical practice. The endpoint of this study was to demonstrate the reliability of a triple method which combines evaluation of: 1. p16 protein expression by immunohistochemistry (p16-IHC); 2. HPV-DNA genotyping by consensus HPV-DNA PCR methods (Consensus PCR); and 3 viral integration into the host by in situ hybridization method (ISH). This triple method has been applied to HN-SCC originated from oral cavity (OSCC) and oropharynx (OPSCC), the two anatomical sites in which high risk (HR) HPVs have been clearly implicated as etiologic factors. Methylation-Specific PCR (MSP) was performed to study inactivation of p16-CDKN2a locus by epigenetic events. Reliability of multiple methods was measured by Kappa statistics. Results: All the HN-SCCs confirmed HPV positive by PCR and/or ISH were also p16 positive by IHC, with the latter showing a very high level of sensitivity as single test (100% in both OSCC and OPSCC) but lower specificity level (74% in OSCC and 93% in OPSCC). Concordance analysis between ISH and Consensus PCR showed a faint agreement in OPSCC ( = 0.38) and a moderate agreement in OSCC ( = 0.44). Furthermore, the addition of double positive score (ISHpositive and Consensus PCR positive) increased significantly the specificity of HR-HPV detection on formalin-fixed paraffin embedded (FFPE) samples (100% in OSCC and 78.5% in OPSCC), but reduced the sensitivity (33% in OSCC and 60% in OPSCC). The significant reduction of sensitivity by the double method was compensated by a very high sensitivity of p16-IHC detection in the triple approach.

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Two types of squamous cell carcinoma of the palatine tonsil characterized by distinct etiology, molecular features and outcome

Cited by 64 publications

References 29 publications

Incidence of Low Risk Human Papillomavirus in Oral Cancer: A Real Time PCR Study on 278 Patients

Incidence of Low Risk Human Papillomavirus in Oral Cancer: A Real Time PCR Study on 278 Patients

Prognostic Indicators in Head and Neck Oncology Including the New 7th Edition of the AJCC Staging System

Evaluation of a combined triple method to detect causative HPV in oral and oropharyngeal squamous cell carcinomas: p16 Immunohistochemistry, Consensus PCR HPV-DNA, and In Situ Hybridization

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