Two types of normal human breast epithelial cells derived from reduction mammoplasty: phenotypic characterization and response to SV40 transfection

Kao, C. Y.; Nomata, Koichiro; Oakley, Cory S.; Welsch, Clifford W.; Chang, Chia‐Cheng

doi:10.1093/carcin/16.3.531

Cited by 142 publications

(203 citation statements)

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“…Specifically, Petersen and colleagues showed that clonal culture of luminal epithelial cells in the presence of cAMP-elevating cholera toxin resulted in the focal conversion of luminal epithelial cells into basal-like cells (Petersen and van Deurs 1988). This finding was confirmed and elaborated in a series of independent studies, and it was interpreted as evidence for stem cell activity (Kao et al 1995;Huper and Marks 2007). That cells converted by cAMP treatment indeed represent cells of the basal lineage was shown by immunomagnetical sorting of the cells based on surface markers and proteomics studies (Péchoux et al 1999).…”

Section: The Identity Of Breast Epithelial Stem Cellsmentioning

confidence: 88%

Stem Cells in the Human Breast

Petersen¹,

Polyák²

2010

Cold Spring Harbor Perspectives in Biology

100

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Section: The Identity Of Breast Epithelial Stem Cellsmentioning

confidence: 88%

Stem Cells in the Human Breast

Petersen¹,

Polyák²

2010

Cold Spring Harbor Perspectives in Biology

100

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“…Type I cells express luminal epithelial markers and stem cell characteristics by being able to both differentiate into other cell types and to form budding structures on reconstituted basement membrane (rBM). Type II cells exhibit a myoepithelial phenotype and form spherical organoids in rBM (38). Interestingly, only type I cells could convert to type II cells not type II to type I (38).…”

Section: The Origin Of Myoepithelial Progenitor Cellsmentioning

confidence: 97%

“…Type II cells exhibit a myoepithelial phenotype and form spherical organoids in rBM (38). Interestingly, only type I cells could convert to type II cells not type II to type I (38). Thus it seems reasonable to conclude that the luminal epithelial and myoepithelial cell lineages are related, and that luminal epithelial cells give rise to myoepithelial cells, at least in the adult gland.…”

Section: The Origin Of Myoepithelial Progenitor Cellsmentioning

confidence: 98%

Myoepithelial Cells: Their Origin and Function in Breast Morphogenesis and Neoplasia

Gudjonsson

Adriance

Sternlicht

et al. 2005

J Mammary Gland Biol Neoplasia

229

211

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The human breast epithelium is a branching ductal system composed of an inner layer of polarized luminal epithelial cells and an outer layer of myoepithelial cells that terminate in distally located terminal duct lobular units (TDLUs). While the luminal epithelial cell has received the most attention as the functionally active milk-producing cell and as the most likely target cell for carcinogenesis, attention on myoepithelial cells has begun to evolve with the recognition that these cells play an active part in branching morphogenesis and tumor suppression. A major question that has been the subject of investigation pertains to how the luminal epithelial and myoepithelial lineages are related and precisely how they arise from a common putative stem cell population within the breast. Equally important is the question of how heterotypic signaling occurs between luminal epithelial and surrounding myoepithelial cells in normal breast morphogenesis and neoplasia. In this review we discuss data from our laboratories and from others regarding the cellular origin of human myoepithelial cells, their function in maintaining tissue polarity in the normal breast, and their role during neoplasia.

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“…19 20 The high proliferative capacity of these cells, together with this uncommitted differentiation state, has led many observers to regard them as being derived from a small "stem cell" pool present in the basal layer of the normal gland. 21 In contrast, most normal breast epithelial cells present in the intact gland and in early passage mammoplasty cultures express CK8, CK18 (weakly), and CK19, but not vimentin. 20 "It is now accepted that the inactivation of the p53 gene, as a result of mutation, is a key step in neoplastic transformation and progression" Although several studies have investigated the expression of CKs and vimentin, EGFR, ER, and the progesterone receptor (PgR) in breast cancer, 17 18 22-30 to our knowledge, no study has directly compared p53 mutations with these phenotypic and differentiation markers in the same case.…”

mentioning

confidence: 94%

p53 mutation in breast cancer. Correlation with cell kinetics and cell of origin

Megha

Ferrari²,

Benvenuto³

et al. 2002

Journal of Clinical Pathology

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Aim: Several studies have investigated the expression of the cytokeratins (CKs), vimentin, the epithelial growth factor receptor (EGFR), the oestrogen receptor (ER), and the progesterone receptor (PgR), in breast cancer, but no study has directly compared p53 mutations with these phenotypic and differentiation markers in the same case. The present study was designed to provide some of this information. Methods: The expression of the p53 and bcl-2 proteins was evaluated by immunohistochemistry in relation to phenotypic characteristics and cellular kinetic parameters (mitotic index and apoptotic index) in 37 cases of ductal carcinoma in situ (DCIS) and 27 cases of infiltrating ductal carcinoma (IDC) of the breast. In addition, p53 gene mutation was examined by polymerase chain reaction single strand conformation polymorphism analysis (SSCP). Results: Thirteen cases (eight DCIS and five IDC) showed expression of CK8, CK14, CK18, vimentin, and EGFR, consistent with a stem cell phenotype, whereas 44 cases (27 DCIS and 17 IDC) showed expression of CK8 and CK1, weak or negative expression of CK18, but were negative for vimentin and EGFR, consistent with a luminal cell phenotype. DCIS and IDC cases with a stem cell phenotype were ER/PgR negative and intermediately or poorly differentiated. In contrast, the cases with luminal cell phenotype were ER/PgR positive and well or intermediately differentiated. In addition, intermediately or poorly differentiated cases with a stem cell phenotype showed higher proliferative activity (per cent of MIB-l positive cells) than did intermediately or well differentiated cases with a luminal cell phenotype. Both DCIS and IDC cases with a stem cell phenotype were p53 positive and bcl-2 negative by immunohistochemistry. In IDC, p53 expression was associated with a reduction of both mitotic index and apoptotic index compared with DCIS. Most of the tumours showing a more differentiated phenotype (luminal) were p53 negative and bcl-2 positive. In these cases, cell kinetic parameters increased from DCIS to IDC. These data suggest the existence of subsets of DCIS and IDC that, because of their phenotypic characteristics, could be derived from subpopulations of normal breast cells having different control mechanisms of cell proliferation and neoplastic progression. Conclusions: These results are compatible with the hypothesis that the phenotype of the cell of origin constrains both tumour phenotype and the choice of genetic events; however, the occurrence of p53 mutants by chance during neoplastic transformation cannot be excluded.

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Two types of normal human breast epithelial cells derived from reduction mammoplasty: phenotypic characterization and response to SV40 transfection

Cited by 142 publications

References 0 publications

Stem Cells in the Human Breast

Stem Cells in the Human Breast

Myoepithelial Cells: Their Origin and Function in Breast Morphogenesis and Neoplasia

p53 mutation in breast cancer. Correlation with cell kinetics and cell of origin

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