Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis

Liu, Qiang; Kasuga, Mie; Sakuma, Yoh; Abe, Hiroshi; Miura, Setsuko; Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo

doi:10.1105/tpc.10.8.1391

Cited by 2,628 publications

(2,669 citation statements)

References 35 publications

(38 reference statements)

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“…Among the DRE binding proteins, the DREB2 subfamily has been reported to be induced by drought and high salinity stress. Transgenic Arabidopsis plants expressing DREB1 B/ CBF1 or DREB1 A/CBF3 under the control of CaMV 35S promoter showed strong tolerance to freezing, drought and high salinity stresses suggesting that DREBs/CBFs target multiple genes (Jaglo-Ottosen et al 1998;Liu et al 1998;Kasuga et al 1999). Microarray analyses have indicated that these factors target stress inducible genes containing the conserved DRE or DRE-related core motifs in their promoter regions (Maruyama et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Luke

Sathik

Thomas

et al. 2015

Physiol Mol Biol Plants

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In order to meet the ever rising global demand for natural rubber, cultivation of Hevea is being extended to nontraditional regions of India where extreme climatic conditions like drought and low temperature negatively influence the crop performance. In order to ensure maximum productivity, identification of drought tolerant clones of Hevea which can cope up with stress and give better crop yield is essential. Several attempts have been made previously to identify genes that are associated with drought tolerance in Hevea. In the present study, quantitative expression analysis was made using quantitative PCR for seven drought associated transcripts in four clones of Hevea with varying levels of drought tolerance. Among the seven genes studied, Mitogen Activated Protein (MAP) kinase, Myeloblastosis (Myb) transcription factor, C-repeat responsive element/Dehydration Responsive Element (CRT/DRE) binding factor and Nuclear Factor Y subunit A (NFYA) showed a positive association with drought tolerance. Transcripts of ascorbate peroxidase and heat shock protein 70 (HSP 70) did not show any correlation with drought tolerance. Interestingly, catalase gene was found down regulated in all the clones under drought condition. The possible role of these genes based on their level of gene expression in four different clones of Hevea with varying levels of drought tolerance is discussed.

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Section: Resultsmentioning

confidence: 99%

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Luke

Sathik

Thomas

et al. 2015

Physiol Mol Biol Plants

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“…It is generally accepted that activation of a specific transcription factor can often result in expression of many functional genes related to stresses. Many efforts have been reported to improve stress resistance of plants by over-expressing stress-responsive transcription factor genes such as DREB/ CBF (Liu et al 1998), ABF2 (Kim et al 2004), OSISAP1 (Mukhopadhyay et al 2004) and etc.…”

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confidence: 99%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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Plants respond to adverse environment by initiating a series of signaling processes including activation of transcription factors that can regulate expression of arrays of genes for stress response and adaptation. NAC (NAM, ATAF, and CUC) is a plant specific transcription factor family with diverse roles in development and stress regulation. In this report, a stress-responsive NAC gene (SNAC2) isolated from upland rice IRA109 (Oryza sativa L. ssp japonica) was characterized for its role in stress tolerance. SNAC2 was proven to have transactivation and DNA-binding activities in yeast and the SNAC2-GFP fusion protein was localized in the rice nuclei. Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment. The SNAC2 gene was over-expressed in japonica rice Zhonghua 11 to test the effect on improving stress tolerance. More than 50% of the transgenic plants remained vigorous when all WT plants died after severe cold stress (4-8°C for 5 days). The transgenic plants had higher cell membrane stability than wild type during the cold stress. The transgenic rice had significantly higher germination and growth rate than WT under high salinity conditions. Over-expression of SNAC2 can also improve the tolerance to PEG treatment. In addition, the SNAC2-overexpressing plants showed significantly increased sensitivity to ABA. DNA chip profiling analysis of transgenic plants revealed many up-regulated genes related to stress response and adaptation such as peroxidase, ornithine aminotransferase, heavy metal-associated protein, sodium/hydrogen exchanger, heat shock protein, GDSL-like lipase, and phenylalanine ammonia lyase. Interestingly, none of the up-regulated genes in the SNAC2-overexpressing plants matched the genes up-regulated in the transgenic plants over-expressing other stress responsive NAC genes reported previously. These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.

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“…However, constitutive expression of several stress-related genes, including CBF1, DREB1A, ATHB7 and yeast trehalose synthase, has resulted in slow growth of transgenic plants (Liu et al, 1998;Holmstrom et al, 2000) and therefore the need for comprehensive evaluation of the transformants arises. Hence, various transgenic plants should be comparatively assessed under identical test conditions to identify the most useful genes.…”

Section: Prospects For Genetic Engineeringmentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis

Cited by 2,628 publications

References 35 publications

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

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