Two temperature-induced changes in mitochondrial membranes detected by spin labelling and enzyme kinetics

Raison, John K.; McMurchie, Edward J.

doi:10.1016/0005-2736(74)90053-4

Cited by 103 publications

(60 citation statements)

References 13 publications

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“…Paramagnetic spin labcls, when incorporated into biological membranes, provide information about the fluidity of their environment (Gaffney, 1974 (Lyons and Raison, 1970;Wisnieski et al, 1971;McMurchie et al, 1973;Raison and McMurchie, 1974) and plant cells (Shneyour et al, 1973;. We used 12NS to determine if the thylakoid membrane lipids in our samples undergo phase transitions that could be correlated with the delayed light emission Arrhenius plot discontinuities.…”

Section: Fatty Acid Spin Labelling Of Thylakoid Membrane and Wuter DImentioning

confidence: 99%

TEMPERATURE DEPENDENCE OF DELAYED LIGHT EMISSION IN THE 6 to 340 MICROSECOND RANGE AFTER A SINGLE FLASH IN CHLOROPLASTS

Jursinic

1977

Photochem & Photobiology

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Abstract-The delayed light emission decay rate (up to 120~s) and the rise in chlorophyll a fluorescence yield (from 3 to 35 ys) in isolated chloroplasts from several species, following a saturating 10 ns flash, are temperature independent in the CL35"C range. However, delayed light in the 120-340ps range is temperature dependent. Arrhenius plots of the exponential decay constants are: (a) linear for lettuce and pea chloroplasts but discontinuous for bush bean (12-17°C) and spinach (12-20°C) chloroplasts; (b) unaffected by 3-(3,4 dich1orophenyl)l ,1-dimethylurea (inhibitor of electron flow), gramicidin D (which eliminates light-induced membrane potential) and glutaraldehyde fixation (which stops gross structural changes).The discontinuities, noted above for bush bean and spinach chloroplasts, are correlated with abrupt changes in (a) the thylakoid membrane lipid fluidity (monitored by EPR spectra of 12 nixtroxide stearate, 12 NS) and (b) the fluidity of extracted lipids (monitored by differential calorimetry and EPR spectra of 12 NS). However, no such discontinuity was observed in (a) chlorophyll a fluorescence intensity of thylakoids and (b) fluorescence of tryptophan residues of delipidated chloroplasts.Microsecond delayed light is linearly dependent on light intensity at flash intensities as low as one quantum per 2 x lo4 chlorophyll molecules. We suggest that this delayed light could originate from a one quantum process in agreement with the hypothesis that recombination of primary charges leads to this light emission. A working hypothesis for the energy levels of Photosystem I1 componcnts is proposed involving a charge stabilization step on the primary acceptor side, which is in a lipid environment.Finally, the redox potential of P680 (the reaction center for chlorophyll of system 11) is calculated to he close to 1.&1.3V.

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Section: Fatty Acid Spin Labelling Of Thylakoid Membrane and Wuter DImentioning

confidence: 99%

TEMPERATURE DEPENDENCE OF DELAYED LIGHT EMISSION IN THE 6 to 340 MICROSECOND RANGE AFTER A SINGLE FLASH IN CHLOROPLASTS

Jursinic

1977

Photochem & Photobiology

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“…The discontinuity in the Arrhenius plot seen with original submitochondrial particles containing very unsaturated endogenous lipids [44,45] and in the dioleoyl-phosphatidylglycerol-stimulated ATPase complex, are of difficult interpretation. It is possible that immobilization of phospholipid by proteins through polar and apolar interaction results in a change of the transition temperature [46] but the association of protein with lipids heterogeneously distributed within the membrane [47,36], the influence of upper and lower limits of transition [48], the cluster formation [37] or a change in the structure of water [49] near the ATPase are other possibilities which deserve consideration.…”

Section: Temperature-induced Changesmentioning

confidence: 99%

The role of phospholipid acyl chains in the activation of mitochondrial ATPase complex

Bruni

Dijck

Gier

1975

Biochimica et Biophysica Acta (BBA) - Biomembranes

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SUMMARY1. The role of length and unsaturation of phospholipid acy! chains in the activation of ATPase complex was studied with synthetic phosphatidylcholines and a phospholipid-dependent preparation obtained after cholate-extraction of submitochondrial particles (Kagawa, Y. and Racker, E. (1966) J. Biol. Chem. 241, 2467-2474.2. Micelle-forming, short-chain phosphatidylcholines produced activation only at critical micellar concentration. The reactivated complex was cold-stable but the oligomycin sensitivity was low.3. Bilayer-forming saturated phosphatidylcholines produced activation which was maximal at 9 carbon atoms in each chain but decreased sharply as the chainlength was increased and essentially disappeared at 14 carbon atoms. By contrast the oligomycin-sensitivity increased with the increase in chain length.4. Activation of ATPase complex reappeared when bilayers were formed with long-chain unsaturated phosphatidylcholines. The activity was oligomycin sensitive. Significant inhibition of activity was observed also after incorporation of cholesterol into the bilayers.5. By contrast the activation induced by negatively charged liposomes of diacylphosphatidyiglycerol was independent on acyi-chain composition and occurred at very low amounts of phospholipid.6. The discontinuity in the Arrhenius plot of activity of the ATPase complex reactivated with saturated phospholipids was found at temperatures close to the gelto-liquid crystalline transition of the lipid showing that the activity of ATPase complex was sensitive to the physical state of membrane phospholipids.7. It is concluded that (a) reactivation of ATPase complex by isoelectric phospholipids is an interracial activation, the minimum requirement for the lipid effect being micelle formation. (b) In order to gain the properties of the native complex a stable lamellar phase is needed. Both activity and oligomycin sensitivity are regulated by the chain length and degree of unsaturation of phospholipid acyl chains.

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“…Chilling injury appeared to be directly related to length of exposure to chilling and inversely related to temperature (Ladaniya and Sonkar 1996). Changes in cell membrane lipids, physical phase and peroxidation are reported to cause chilling (Eaks and Raison 1970;Raison et al 1971;Shewfelt and Purvis 1995). However, the mechanism of chilling and factors and biochemical processes responsible for development of chilling injury are not yet fully known.…”

Section: Introductionmentioning

confidence: 99%

Physico−chemical, respiratory and fungicide residue changes in wax coated mandarin fruit stored at chilling temperature with intermittent warming

Ladaniya

2010

J Food Sci Technol

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Influence of chilling temperature, intermittent warming (IW) and fungicidal wax coating was evaluated during storage of 'Nagpur' mandarins (Citrus reticulata Blanco). Fruits were light green coloured with slight colour-break at the start of storage. Waxed and non-waxed fruits were stored at 3.5°C (constant), 2 weeks at 3.5°C followed by IW for 1 week at 19.5°C (cycle) and at 6.5°C (constant), and were evaluated immediately after 30, 45, 60, 75 days and also after 1 week holding at ambient condition (24±2°C, 60-70% RH). There was no chilling injury to fruit under IW treatment irrespective of coating. At 3.5°C (constant) chilling injury appeared after 45 days during 1 week holding and thereafter increased at each storage interval. Wax coated fruit had lower chilling injury. Fruit under IW treatment and at 6.5°C (constant) developed yellow-orange colour while at 3.5°C (constant) fruit remained green during storage. Juice content, titratable acidity and ascorbic acid contents were not affected by temperature regimes and waxing while total soluble solids content was higher with IW treatment. Reducing and total sugars were higher in fruits stored at IW treatment and at 6.5°C (constant) than at 3.5°C (constant). Total peel phenols content were not significantly affected by waxing and temperature regimes. However, loss of phenols content was higher at 3.5°C (constant). Phenol content decreased during storage. At 3.5°C (constant), chlorophyll ('a', 'b' and total) content in peel was maximum while total carotenoids were minimum with little colour development. Rapid colour development was recorded under IW and also at 1 week holding. Wax coating delayed colour development at 3.5°C (constant). Initially carbendazim residues were higher in peel (4.0 ppm) and pulp (3.2 ppm) of waxed fruit than in non-waxed (3.2 ppm in peel and 3.1 ppm in pulp) fruit. Overall drop in residues till storage up to 75 days+ 1 week over the initial values was 80.2-85.6% in peel and 56.2-75.8% in pulp of waxed and non-waxed fruit, respectively. Respiration was lower in waxed fruit. Respiratory rate was lowest at 3.5°C (constant) and it changed with IW. At 3.5°C and 6.5°C (constant), range of respiration was 4-6 mgCO 2 /kg/h and 7-9 mgCO 2 /kg/h, respectively in waxed and non-waxed fruit. Respiratory rate increased as the fruit was removed to warmer temperature. Chilling injury caused considerable rise in respiration rate of fruit. Present findings indicated that storage life of 'Nagpur' mandarin can be extended up to 75 days at 3.5°C with IW.

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Two temperature-induced changes in mitochondrial membranes detected by spin labelling and enzyme kinetics

Cited by 103 publications

References 13 publications

TEMPERATURE DEPENDENCE OF DELAYED LIGHT EMISSION IN THE 6 to 340 MICROSECOND RANGE AFTER A SINGLE FLASH IN CHLOROPLASTS

TEMPERATURE DEPENDENCE OF DELAYED LIGHT EMISSION IN THE 6 to 340 MICROSECOND RANGE AFTER A SINGLE FLASH IN CHLOROPLASTS

The role of phospholipid acyl chains in the activation of mitochondrial ATPase complex

Physico−chemical, respiratory and fungicide residue changes in wax coated mandarin fruit stored at chilling temperature with intermittent warming

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