Two Strains of Lactobacilli Effectively Decrease the Colonization of VRE in a Mouse Model

Li, Xianping; Song, Lihua; Zhu, Siyi; Xiao, Yuchun; Huang, Yuanming; Hua, Yuting; Chu, Qiongfang; Ren, Zhihong

doi:10.3389/fcimb.2019.00006

Cited by 17 publications

(15 citation statements)

References 51 publications

(57 reference statements)

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“…The r-values are denoted with graduated colors, and red and blue grids indicate positive and negative correlations, respectively. "ns" indicates nonsignificance; * P < 0.05, * * P < 0.01, * * * P < 0.001. not increase the abundance of Lactobacilli and Bifidobacterium (Supplementary Figure S2) in feces and similar result was observed in our previous study (Li et al, 2019). Intake of probiotics mixture may have an effect on gut microbiota and bile acids by their metabolites as transient stay of them in intestine, which would be confirmed in the future study.…”

Section: Discussionsupporting

confidence: 90%

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

Chu

Huang

et al. 2019

Front. Microbiol.

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Clostridioides difficile infection (CDI) is increasing morbidity and mortality rates globally. Fecal microbiota transplantation (FMT), an effective therapy for eliminating Clostridioides difficile (C. difficile), cannot be used extensive due to a range of challenges. Probiotics thus constitutes a promising alternative therapy. In our study, we evaluated the effect of consortium of probiotics including five Lactobacilli strains and two Bifidobacterium strains on the colonization of toxigenic BI/NAP1/027 C. difficile in a mouse model. The results of 16S rRNA sequencing and targeted metabolomics showed the consortium of probiotics effectively decreased the colonization of C. difficile, changed the αand β-diversity of the gut microbiota, decreased the primary bile acids, and increased the secondary bile acids. Spearman's correlation showed that some of the OTUs such as Akkermansia, Bacteroides, Blautia et al. were positively correlated with C. difficile numbers and the primary bile acids, and negatively correlated with the secondary bile acids. However, some of the OTUs, such as Butyricicoccus, Ruminococcus, and Rikenellaceae, were negatively correlated with C. difficile copies and the primary bile acids, and positively correlated with the secondary bile acids. In summary, the consortium of probiotics effectively decreases the colonization of C. difficile, probably via alteration of gut microbiota and bile acids. Our probiotics mixture thus offers a promising FMT alternative.

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Section: Discussionsupporting

confidence: 90%

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

Chu

Huang

et al. 2019

Front. Microbiol.

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“…It is also possible that the BTs modified the nasal microbiota in a way that conferred increased colonization resistance to M. haemolytica. A previous study showed that microbiota manipulation using probiotic Lactobacillus strains reduced the colonization of vancomycin-resistant Enterococcus (VRE) in challenged mice (26). The authors proposed that the compositional alteration observed from phylum to species level by probiotic strains may have contributed to decreased VRE colonization.…”

Section: Discussionmentioning

confidence: 99%

Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

et al. 2020

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Six Lactobacillus strains originating from the nasopharyngeal microbiota of cattle were previously characterized in vitro and identified as candidate bacterial therapeutics (BTs) for mitigating the bovine respiratory pathogen Mannheimia haemolytica. In the present study, these BT strains were evaluated for their potential to (i) reduce nasal colonization by M. haemolytica, (ii) modulate the nasal microbiota, and (iii) stimulate an immune response in calves experimentally challenged with M. haemolytica. Twenty-four Holstein bull calves (1 to 3 weeks old) received either an intranasal BT cocktail containing 6 Lactobacillus strains (3 × 109 CFU per strain; BT + Mh group) 24 h prior to intranasal M. haemolytica challenge (3 × 108 CFU) or no BTs prior to challenge (Mh, control group). Nasal swab, blood, and transtracheal aspiration samples were collected over the course of 16 days after BT inoculation. Counts of M. haemolytica were determined by culturing, and the nasal and tracheal microbiotas were evaluated using 16S rRNA gene sequencing. Serum cytokines (interleukin-6 [IL-6], IL-8, and IL-10) were quantified by enzyme-linked immunosorbent assay (ELISA). Administration of BT reduced nasal colonization by M. haemolytica (P = 0.02), modified the composition and diversity of the nasal microbiota, and altered interbacterial relationships among the 10 most relatively abundant genera. The BT + Mh calves also had a lower relative abundance of Mannheimia in the trachea (P < 0.01) but similar cytokine levels as Mh calves. This study demonstrated that intranasal BTs developed from the bovine nasopharyngeal Lactobacillus spp. were effective in reducing nasal colonization by M. haemolytica in dairy calves. IMPORTANCE Bovine respiratory disease (BRD) is one of the significant challenges for the modern dairy industry in North America, accounting for 23 to 47% of the total mortality among pre- and postweaned dairy heifers. Mass medication with antibiotics is a common practice to control BRD in dairy cattle. However, the emergence of multidrug-resistant BRD pathogens highlights the importance of developing alternatives to antibiotics for BRD mitigation. Using a targeted approach, we recently identified 6 Lactobacillus strains originating from the bovine respiratory microbiota as candidates to be used as bacterial therapeutics (BTs) for the mitigation of the BRD pathogen Mannheimia haemolytica. Here, we demonstrated that intranasal inoculation of the BT strains reduced nasal colonization by M. haemolytica in dairy calves experimentally challenged with this pathogen. This study, for the first time, shows the potential use of intranasal BTs as an alternative to mitigate BRD pathogens in cattle.

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“…Fecal microbiota transplantation (FMT) to decolonize VRE can also be regarded as a multiple-species treatment that reduced the colonization of VRE in a mouse model and a clinical trial [55,56]. However, FMT treatment is more invasive and it carries a risk of multiple-drug-resistant pathogenic infections.…”

Section: Discussionmentioning

confidence: 99%

“…The strategy of administering multiple probiotic species, including L. rhamnosus and L. acidophilus , was adopted in another trial; however, it failed to prevent the colonization of ampicillin-resistant E. faecium [ 54 ], which suggested that MNA-assisted, in vitro study-supported selection of a mixture of multiple probiotics can be more promising in clinical trials. Fecal microbiota transplantation (FMT) to decolonize VRE can also be regarded as a multiple-species treatment that reduced the colonization of VRE in a mouse model and a clinical trial [ 55 , 56 ]. However, FMT treatment is more invasive and it carries a risk of multiple-drug-resistant pathogenic infections.…”

Section: Discussionmentioning

confidence: 99%

Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci

et al. 2020

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The prevalence of vancomycin resistant enterococcus (VRE) carrier-state has been increasing in patients of intensive care unit and it would be a public health threat. Different research groups conducted decolonizing VRE with probiotic and the results were controversial. Therefore, a systemic approach to search for the probiotic species capable of decolonizing VRE is necessary. Thus, VRE was co-cultured with ten probiotic species. The fluctuations of each bacterial population were analyzed by 16S rRNA sequencing. Microbial network analysis (MNA) was exploited to identify the most critical species in inhibiting the VRE population. The MNA-selected probiotic cocktail was then validated for its efficacy in inhibiting VRE, decolonizing VRE from Caco-2 cells via three approaches: exclusion, competition, and displacement. Finally, the expression of VRE virulence genes after co-incubation with the probiotic cocktail were analyzed with quantitative real-time PCR (qRT-PCR). The MNA-selected probiotic cocktail includes Bacillus coagulans, Lactobacillus rhamnosus GG, Lactobacillus reuteri, and Lactobacillus acidophilus. This probiotic combination significantly reduces the population of co-cultured VRE and prevents VRE from binding to Caco-2 cells by down-regulating several host-adhesion genes of VRE. Our results suggested the potential of this four-strain probiotic cocktail in clinical application for the decolonization of VRE in human gut.

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Two Strains of Lactobacilli Effectively Decrease the Colonization of VRE in a Mouse Model

Cited by 17 publications

References 51 publications

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci

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