Two-step microalgal (Coelastrella sp.) treatment of raw piggery wastewater resulting in higher lipid and triacylglycerol levels for possible production of higher-quality biodiesel

Lee, Sang‐Ah; Ko, So-Ra; Lee, Nakyeong; Lee, Dong Kun; Le, Ve Van; Oh, Hee‐Mock; Ahn, Chi‐Yong

doi:10.1016/j.biortech.2021.125081

Cited by 28 publications

(16 citation statements)

References 46 publications

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“…Zhu et al [40] optimized the growth of Chlorella zofingiensis in diluted piggery wastewater (initial COD, 1900 mg L −1 ). However, this study utilized undiluted piggery wastewater according to our previous studies [11,21], and the initial concentrations of COD (3100 mg L −1 ) and ammonia-nitrogen (770 mg L −1 ) were much higher than the usual tolerance range for microalgae. Fernández-Linare et al [41] achieved a higher removal rate at a pilot scale relative to this study, but it required 13 days of cultivation.…”

Section: Discussionmentioning

confidence: 99%

“…To treat raw piggery wastewater efficiently, a two-step (heterotrophic and mixotrophic) microalgal cultivation strategy was employed based on our previous study [21]. For the heterotrophic phase of wastewater treatment, pilot-scale PBRs were covered with aluminum foil to block natural sunlight, and then fresh raw piggery wastewater from Jacob Farms Corporation in South Chungcheong Province were transferred to the PBRs.…”

Section: Pilot-scale Piggery Wastewater Treatment Using Microalgaementioning

confidence: 99%

“…The present study focused on bacterial community changes during the microalgal treatment of piggery wastewater at a pilot scale. Undiluted raw piggery wastewater was treated using a two-step treatment process developed in our previous study [21]. Amplicon sequencing was used to reveal the shift patterns of the bacterial communities.…”

Section: Graphical Abstractintroductionmentioning

confidence: 99%

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Extra benefit of microalgae in raw piggery wastewater treatment: pathogen reduction

Lee

Kim

et al. 2022

Microbiome

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Background Monitoring microbial communities especially focused on pathogens in newly developed wastewater treatment systems is recommended for public health. Thus, we investigated the microbial community shift in a pilot-scale microalgal treatment system for piggery wastewater. Results Microalgae showed reasonable removal efficiencies for COD and ammonia, resulting in higher transparency of the final effluent. Metagenome and microbial diversity analyses showed that heterotrophic microalgal cultivation barely changed the bacterial community; however, the mixotrophic microalgal cultivation induced a sudden change. In addition, an evaluation of risk groups (RGs) of bacteria showed that raw piggery wastewater included abundant pathogens, and the microalgal treatment of the raw piggery wastewater decreased the RG2 pathogens by 63%. However, co-cultivation of microalgae and the most dominant RG2 pathogen, Oligella, showed no direct effects between them. Conclusions Thus, a microbial interaction network was constructed to elucidate algae-bacteria interrelationships, and the decrease in Oligella was indirectly connected with microalgal growth via Brevundimonas, Sphingopyxis, and Stenotrophomonas. In a validation test, 3 among 4 connecting bacterial strains exhibited inhibition zones against Oligella. Therefore, we showed that microalgal wastewater treatment causes a decrease in RG2 bacteria, which is an indirect impact of microalgae associated with bacteria. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Pilot-scale Piggery Wastewater Treatment Using Microalgaementioning

confidence: 99%

Section: Graphical Abstractintroductionmentioning

confidence: 99%

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Extra benefit of microalgae in raw piggery wastewater treatment: pathogen reduction

Lee

Kim

et al. 2022

Microbiome

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“…NH4Cl understanding in TAG induction in microalga cells can be applied to improve the microalgal lipid production process and environment bioremediation [20]. The ammonium biochemical mechanisms on microalgal growth and triglyceride production were initially investigated under photoautotrophic and mixotrophic conditions.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Role of NH<sub>4</sub>Cl Supplementation on Triacylglycerol Production by <em>Chlamydomonas reinhardtii </em>under Mixotrophic Cultivation using a Proteomics Approach

Sittisaree¹,

Yokthongwattana²,

Aonbangkhen³

et al. 2022

Preprint

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NH4Cl is one of the nitrogen sources for microalgal cultivation. However, excessive amounts of NH4Cl affects microalgal physiology and biomass contents. In this study, the effects of ammonium on microalgal growth and TAG content in the green microalga (Chlamydomonas reinhardtii) was investigated. Microalgal growth and TAG content under photoautotrophic conditions were found to be unchanged with 17 mM of ammonium, while this compound interfered with microalgal growth and induced TAG content under mixotrophic conditions with acetate supplementation. This suggested that ammonium could induce TAG production when acetate occurred in microalgal cultivation. Further, the effects of two different concentrations of NH4Cl (17 mM and 60 mM) on the cells under mixotrophic conditions were investigated. The results showed that both concentrations reduced microalgal growth, but induced total lipid and TAG content, especially after a 4-day cultivation. The oxygen evolution and Fv/Fm ratio showed that both concentrations completely inhibited the oxygen evolution on Day 4. The 60 mM NH4Cl reduced the Fv/Fm ratio from 0.7 to 0.48 indicating that ammonium supplementation directly affects the microalgae photosynthesis performance. A total of 1782 proteins were successfully identified using proteomics analysis. Among them, there were nine overexpressed proteins and four proteins were underexpressed. Using the protein–ligand interaction analysis, nitrogen metabolism is involved under NH4Cl conditions. This information can provide biochemical knowledge for microalgae development for sustainable energy usage.

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“…Os perfis lipídicos apontaram predominância na formação de ácido araquídico (C20:0) seguido do α-linolênico (C18:3). Pelos perfis, nota-se que grande parte dos ácidos graxos que compõem os lipídeos são ácidos graxos saturados, matéria-prima com potencial para geração de biodiesel dadas as seguintes propriedades que conferem ao combustível: alta estabilidade oxidativa, baixo retardo de ignição e menor potencial de emissão de NOx, poluente intensamente agressivo para a camada de ozônio (LEE et al, 2021).…”

Section: Determinação Dos Lipídeos Totaisunclassified

Uso de vinhaça como meio de cultivo de microalgas e obtenção de biomoléculas de interesse biotecnológico.

Silva¹

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Agradeço a Deus por me amparar nos momentos difíceis e minha família SandraAparecida Silva e Orlando Silva, por todo o incentivo e auxilio durante a minha vida sem os quais não seria possível completar mais esta etapa.Agradeço a minha companheira Gisele Barreto Brito por ter me incentivado a cada momento. Agradeço aos meus amigos Marcos Ruberson e Letícia Fernandes por todos os anos de convívio acreditando em mim, dividindo emoções e sempre crédulos nos resultados positivos.Agradeço ao meu filho Valentin Silva Barreto por cada sorriso, olhar, carinho, afeto que me impulsionam a todo segundo.Ao professor Dr. Claúdio Augusto Oller Nascimento, pela oportunidade de estar nesse projeto de aprender e trocar conhecimento, por ter acreditado no meu trabalho; A professora Dra. Elen Aquino Perpetuo, pela recepção no laboratório, pela ajuda nos momentos em que mais precisei, por sua amizade, pois sua participação foi fundamental para a realização deste trabalho;As colegas do CEPEMA,

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Two-step microalgal (Coelastrella sp.) treatment of raw piggery wastewater resulting in higher lipid and triacylglycerol levels for possible production of higher-quality biodiesel

Cited by 28 publications

References 46 publications

Extra benefit of microalgae in raw piggery wastewater treatment: pathogen reduction

Extra benefit of microalgae in raw piggery wastewater treatment: pathogen reduction

Exploring the Role of NH<sub>4</sub>Cl Supplementation on Triacylglycerol Production by <em>Chlamydomonas reinhardtii </em>under Mixotrophic Cultivation using a Proteomics Approach

Uso de vinhaça como meio de cultivo de microalgas e obtenção de biomoléculas de interesse biotecnológico.

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