Two-step formulation of magnetic nanoprobes for microRNA capture

Vilímová, Iveta; Chourpa, Igor; David, Stéphanie; Soucé, Martin; Hervé-Aubert, Katel

doi:10.1039/d1ra09016j

Cited by 6 publications

(11 citation statements)

References 36 publications

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“…A detailed description of the full protocol of the synthesis was described in our previous study. 26 The formulation of nanoprobes is divided into two steps: surface modification of nanoprobes precursors with streptavidin and subsequent functionalization with cDNA. Precursors of the nanoprobes, PEG-b-coated SPIONs, were synthesized in three steps, starting with the synthesis of initial SPIONs, followed by the silanization of their surface, which was then covered with the PEG-b layer using lyophilized NHS−PEG 5000 −biotin (in accordance to the protocol previously developed by our group 27 ).…”

Section: Formulation Of Nanoprobesmentioning

confidence: 99%

“…The core made of the MNPs is made of SPIONs coated with poly(ethylene glycol) (PEG) for better colloidal stability in complex media. In order to ensure the TmiR-484 capture, the PEG chains were decorated, via streptavidin−biotin interaction, with cDNA-484 (in contrast with cmiR that we reported previously 26 ). We tested the MNP stability and ability to capture TmiR-484, first in buffer solutions and then in human serum samples from healthy adult volunteers, where the determined levels of TmiR-484 were generated by spiking and monitored by SE-HPLC.…”

Section: Introductionmentioning

confidence: 99%

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Fe₃O₄-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening

Vilímová,

David,

Vigouroux

et al. 2024

ACS Appl. Nano Mater.

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miRNAs (miRs) are short, noncoding RNA sequences that are present in biofluids such as blood, plasma, and serum. Their disrupted levels of expression correlate with different stages of various diseases, making them suitable as potential biomarkers. In this study, we present magnetic nanoprobes (MNPs) designed to capture specific target miRs (TmiRs) in biofluids like serum. The nanoprobes were formulated starting from PEGylated SPIONs using streptavidin−biotin interactions. The PEG chains were functionalized with complementary DNA sequence (cDNA-484) able to bind to TmiR-484, which is known to be overexpressed in serum of patients with colorectal cancer. The two-step formulation of the MNPs was monitored by SE-HPLC, following a previously established protocol. The colloidal stability of the MNPs and their ability to capture TmiR-484 were first determined in model solutions and then in human serum enriched with the commercially purchased TmiR-484. After magnetic sorting, the TmiR was released from the MNPs (dehybridization step) and quantified using qRT-PCR.

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Section: Formulation Of Nanoprobesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fe₃O₄-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening

Vilímová,

David,

Vigouroux

et al. 2024

ACS Appl. Nano Mater.

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“…Finally, polymeric-based nanoparticles can be difficult to scale up for industrial applications, as the reaction conditions must be carefully monitored. Doxorubicin-loaded protease-activated near-infrared fluorescent polymeric nanoparticles have been used for imaging and therapy for cancer [ 49 ]. In another study, the biodistribution of 99mTc-PLA/PVA/Atezolizumab nanoparticles has been used for the diagnosis of non-small cell lung cancer [ 50 ].…”

Section: Commonly Used Nanoparticles In Cancer Diagnosismentioning

confidence: 99%

Nanotechnology in Cancer Diagnosis and Treatment

et al. 2023

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Traditional cancer diagnosis has been aided by the application of nanoparticles (NPs), which have made the process easier and faster. NPs possess exceptional properties such as a larger surface area, higher volume proportion, and better targeting capabilities. Additionally, their low toxic effect on healthy cells enhances their bioavailability and t-half by allowing them to functionally penetrate the fenestration of epithelium and tissues. These particles have attracted attention in multidisciplinary areas, making them the most promising materials in many biomedical applications, especially in the treatment and diagnosis of various diseases. Today, many drugs are presented or coated with nanoparticles for the direct targeting of tumors or diseased organs without harming normal tissues/cells. Many types of nanoparticles, such as metallic, magnetic, polymeric, metal oxide, quantum dots, graphene, fullerene, liposomes, carbon nanotubes, and dendrimers, have potential applications in cancer treatment and diagnosis. In many studies, nanoparticles have been reported to show intrinsic anticancer activity due to their antioxidant action and cause an inhibitory effect on the growth of tumors. Moreover, nanoparticles can facilitate the controlled release of drugs and increase drug release efficiency with fewer side effects. Nanomaterials such as microbubbles are used as molecular imaging agents for ultrasound imaging. This review discusses the various types of nanoparticles that are commonly used in cancer diagnosis and treatment.

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“…However, with small biomolecules such as miRNA, the changes in DH and zeta potential are generally too small to be reliable proof of conjugation. Although DLS and zetametry measurements are very common and can be found in the majority of performed studies, they are also necessarily joined by other complementary techniques which allow for more accurate confirmations [ 50 ].…”

Section: Nanomaterials Used As Substratesmentioning

confidence: 99%

“…In our previous publication, we modified the surface of PEGylated SPOINs with streptavidin. Biotin at the end of the PEG chain allowed close coverage of streptavidin, and the creation of the sufficient streptavidin layer of available biotin-binding spots enabled the functionalization with biotinylated complementary miRNA sequences, leading to the formation of nanoprobes with the ability to capture the target miRNA [ 50 ].…”

Section: Conjugation Strategies Leading To Functionalizationmentioning

confidence: 99%

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

Vilímová¹,

Hervé-Aubert²,

Chourpa³

2022

Molecules

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Recently, microRNAs (miRNA) captured the interest as novel diagnostic and prognostic biomarkers, with their potential for early indication of numerous pathologies. Since miRNA is a short, non-coding RNA sequence, the sensitivity and selectivity of their detection remain a cornerstone of scientific research. As such, methods based on nanomaterials have emerged in hopes of developing fast and facile approaches. At the core of the detection method based on nanotechnology lie nanoprobes and other functionalized nanomaterials. Since miRNA sensing and detection are generally rooted in the capture of target miRNA with the complementary sequence of oligonucleotides, the sequence needs to be attached to the nanomaterial with a specific conjugation strategy. As each nanomaterial has its unique properties, and each conjugation approach presents its drawbacks and advantages, this review offers a condensed overview of the conjugation approaches in nanomaterial-based miRNA sensing. Starting with a brief recapitulation of specific properties and characteristics of nanomaterials that can be used as a substrate, the focus is then centered on covalent and non-covalent bonding chemistry, leading to the functionalization of the nanomaterials, which are the most commonly used in miRNA sensing methods.

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Two-step formulation of magnetic nanoprobes for microRNA capture

Abstract: Two-step formulation of magnetic nanoprobes for microRNA capture.

Cited by 6 publications

References 36 publications

Fe₃O₄-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening

Fe₃O₄-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening

Nanotechnology in Cancer Diagnosis and Treatment

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

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Two-step formulation of magnetic nanoprobes for microRNA capture

Abstract: Two-step formulation of magnetic nanoprobes for microRNA capture.

Cited by 6 publications

References 36 publications

Fe3O4-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening

Fe3O4-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening

Nanotechnology in Cancer Diagnosis and Treatment

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

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Fe₃O₄-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening

Fe₃O₄-Based Magnetic Nanoprobes for miRNA-484 Capture in Human Serum: Implications for Colorectal Cancer Screening