Two Stem Cell Populations Including VSELs and CSCs Detected in the Pericardium of Adult Mouse Heart

Bhartiya, Deepa; Flora, Yash; Sharma, Diksha; Mohammad, Subhan Ali

doi:10.1007/s12015-021-10119-9

Cited by 5 publications

(5 citation statements)

References 60 publications

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“…Nevertheless, the BM stem cell compartment still needs to be characterized better. To support this, our team described rare stem cells with broader, cross-germ layer speci cation potential that were named very small embryonic-like stem cells (VSELs) [6][7][8][9][10][11][12][13][14]. Subsequently, several independent laboratories have con rmed the presence of these cells in postnatal tissues [6][7][8][9][10][11][12][13][14].…”

Section: Discussionmentioning

confidence: 99%

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Murine and human-purified very small embryonic-like stem cells (VSELs) express purinergic receptors and migrate to extracellular ATP gradient.

Bujko,

Brzezniakiewicz-Janus,

Jarczak

et al. 2024

Preprint

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Purinergic signaling is an ancient primordial signaling system regulating tissue development and specification of various types of stem cells. Thus, functional purinergic receptors are present in several types of cells in the body, including multiple populations of stem cells. However, one stem cell type that has not been evaluated for expression of purinergic receptors is very small embryonic stem cells (VSELs) isolated from postnatal tissues. Herein, we report that VSELs purified human umbilical cord blood (UCB) and murine bone marrow (BM) express mRNA for P1 and P2 purinergic receptors and CD39 and CD73 ectonucleotidases converting extracellular ATP (eATP) into its signaling metabolite extracellular adenosine (eAdo), that antagonizes eATP effects. More importantly, we demonstrate that human and murine VSELs respond by chemotaxis to eATP, and eAdo inhibits this migration. These responses to eATP are mediated by activation of Nlrp3 inflammasome, and exposure of VSELs to its specific inhibitor MCC950 abolished the chemotactic response to ATP. We conclude that purinergic signaling plays an essential, underappreciated role in the biology of these cells and their potential role in response to tissue/organ injuries.

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Section: Discussionmentioning

confidence: 99%

“…These discoveries challenged the view on the adult stem cell compartment hierarchy. Accordingly, as reported, VSELs may become speci ed, e.g., to hematopoietic cells [9], endothelial progenitors [10], and gametes [11], as well as contribute to the cardiomyocytes [12], hepatocytes [13], and pulmonary alveolar epithelium [14].…”

Section: Introductionmentioning

confidence: 99%

Murine and human-purified very small embryonic-like stem cells (VSELs) express purinergic receptors and migrate to extracellular ATP gradient.

Bujko,

Brzezniakiewicz-Janus,

Jarczak

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…These discoveries challenged the view on the adult stem cell compartment hierarchy. Accordingly, as reported, VSELs may become specified, e.g., to hematopoietic cells [ 9 ], endothelial progenitors [ 10 ], and gametes [ 11 ], as well as contribute to the cardiomyocytes [ 12 ], hepatocytes [ 13 ], and pulmonary alveolar epithelium [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Murine and Human-Purified very Small Embryonic-like Stem Cells (VSELs) Express Purinergic Receptors and Migrate to Extracellular ATP Gradient

Bujko,

Brzezniakiewicz-Janus,

Jarczak

et al. 2024

Stem Cell Rev and Rep

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Purinergic signaling is an ancient primordial signaling system regulating tissue development and specification of various types of stem cells. Thus, functional purinergic receptors are present in several types of cells in the body, including multiple populations of stem cells. However, one stem cell type that has not been evaluated for expression of purinergic receptors is very small embryonic stem cells (VSELs) isolated from postnatal tissues. Herein, we report that human umbilical cord blood (UCB) and murine bone marrow (BM) purified VSELs express mRNA for P1 and P2 purinergic receptors and CD39 and CD73 ectonucleotidases converting extracellular ATP (eATP) into its signaling metabolite extracellular adenosine (eAdo), that antagonizes eATP effects. More importantly, we demonstrate that human and murine VSELs respond by chemotaxis to eATP, and eAdo inhibits this migration. These responses to eATP are mediated by activation of Nlrp3 inflammasome, and exposure of VSELs to its specific inhibitor MCC950 abolished the chemotactic response to ATP. We conclude that purinergic signaling plays an essential, underappreciated role in the biology of these cells and their potential role in response to tissue/organ injuries. Graphical Abstract

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“…The physiological and pathological characteristics of cardiac stem cells have been revisited lately by Öztürk and Elçin [22], and a number of authors have renewed new studies on c-kit cells [23,24]. As shown by Bhartiya's more recent observation [25], the unexpected loss of stem cells during the course of performing different experiments has contributed to the misconceptions and disputes prevailing in the eld of cardiac stem cells, which need to be urgently corrected. With paracrine support from mesenchymal stromal cells, VSELs/CSCs have the potential to regenerate damaged cardiac tissue.…”

Section: Introductionmentioning

confidence: 99%

“…Regardless of the presence or absence of resident stem cells within the myocardium being debated, one indisputable phenomenon is that the following types of cells expressing markers from the embryonic period are present in developing and adult myocardial tissue: Sca-1(stem cell antigen-1)-positive cells [26][27][28][29][30]; Nanog-positive cells [25,31,32]; Islet-1 positive cells [27,[33][34][35][36], previous researchers have often identi ed a positive expression of a marker as representing a subset of cells. However, "stem cell identity" of a cell is not solely associated with a single speci c biomarker, and it is suggested that these phenotypically distinct populations of cells may be simply phenotypic variations of a unique cell type at different stages [28].…”

Section: Introductionmentioning

confidence: 99%

Subpopulation analysis of Sca-1, Nanog, and Islet-1 positive cells in myocardial tissue

Liu

Guo

Dou

et al. 2022

Preprint

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To investigate the co-expression between different stem-cell or embryonic markers in myocardial tissue, which has rarely been discussed in previous studies, we selected three typical molecular markers, namely Sca-1, Nanog and Islet-1. In our study, we focused on the age-related changes of Sca-1, Nanog and Islet-1 expression and the co-localization levels between pin-two marker proteins to explore the heterogeneity and relevance between these three cell subpopulations. We found that Sca-1, Nanog, and Islet-1 positive cells were present in the cardiac tissue from newborn to adult mice, and their expression was negatively correlated with age. Co-localization existed between each two markers, and there were differences in co-localization levels at different stages of cardiac development. We thus hypothesize that these three positive cells are likely to be a group of cells, so that cardiac cells with myocardial differentiation potential should not be subclassified by a single cell marker, and their phenotypic changes at different stages may determine their unique roles in heart development.

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Two Stem Cell Populations Including VSELs and CSCs Detected in the Pericardium of Adult Mouse Heart

Cited by 5 publications

References 60 publications

Murine and human-purified very small embryonic-like stem cells (VSELs) express purinergic receptors and migrate to extracellular ATP gradient.

Murine and human-purified very small embryonic-like stem cells (VSELs) express purinergic receptors and migrate to extracellular ATP gradient.

Murine and Human-Purified very Small Embryonic-like Stem Cells (VSELs) Express Purinergic Receptors and Migrate to Extracellular ATP Gradient

Subpopulation analysis of Sca-1, Nanog, and Islet-1 positive cells in myocardial tissue

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