2010
DOI: 10.1116/1.3431524
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Two stages in three-dimensional in vitro growth of tissue generated by osteoblastlike cells

Abstract: Bone regeneration is controlled by a variety of biochemical, biomechanical, cellular, and hormonal mechanisms. In particular, physical properties of the substrate such as stiffness and architecture highly influence the proliferation and differentiation of cells. The aim of this work is to understand the influence of scaffold stiffness and cell seeding densities on the formation of tissue by osteoblast cells within polyether urethane scaffolds containing pores of different sizes. MC3T3-E1 preosteoblast cells we… Show more

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Cited by 52 publications
(44 citation statements)
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“…In line with our findings of correlating ECM amount and 3D tissue growth, an osteogenic cell line MCT3T-E1 previously used in similar experiments [35,36,42,52] was observed to secrete a very high amount of collagen (compared to MSC, data not shown) and subsequently rapidly created a stable 3D tissue-like structures inside various shaped macroscopic channels [39]. Nevertheless, both cell types, MC3T3-E1 and osteogenic differentiated MSC, showed an initial linear growth phase which turned into a plateau phase of growth after about 14 days.…”
Section: Discussionsupporting
confidence: 89%
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“…In line with our findings of correlating ECM amount and 3D tissue growth, an osteogenic cell line MCT3T-E1 previously used in similar experiments [35,36,42,52] was observed to secrete a very high amount of collagen (compared to MSC, data not shown) and subsequently rapidly created a stable 3D tissue-like structures inside various shaped macroscopic channels [39]. Nevertheless, both cell types, MC3T3-E1 and osteogenic differentiated MSC, showed an initial linear growth phase which turned into a plateau phase of growth after about 14 days.…”
Section: Discussionsupporting
confidence: 89%
“…Channel-containing culture carriers were previously used to investigate the influence of substrate geometry on bone like tissue growth [35]. After an initial migration, proliferation and adhesion phase till full coverage of the channel surfaces with cells, tissue growth kinetics in such systems was found independent of substrate characteristics, such as stiffness, most likely because further cell proliferation and differentiation occurs inside the cells own matrix [39]. Tissue growth was also found to be promoted by certain channel geometries [36].…”
Section: Discussionmentioning
confidence: 98%
“…The ingrowth of mineralized tissue into the channel void, as represented by coverage, was directly dependent on curvature. As hypothesized, mineralized tissue formation was increased with increasing curvature, as reported for non-mineralized ECM previously [6,14,15,19]. A limitation of this study is the lack of a cell-free control group.…”
Section: Discussionmentioning
confidence: 80%
“…It was shown that there exists an intimate correlation between geometrical changes and cellular behaviour in vivo [3]. Different experimental studies have shown that curvature influences tissue growth under static and perfused conditions in vitro [6,[13][14][15]19]. Nevertheless, these studies focused on non-mineralized ECM formation only and evaluations of tissue growth were performed in limited three-dimensional volumes.…”
Section: Discussionmentioning
confidence: 99%
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